Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey Cover

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Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey

The EuroBiotech Journal

Volume 5 (2021): Issue 2 (April 2021)

By: Alparslan Yildirim, Ferda Sevinc, Zuhal Onder, Onder Duzlu, Ozlem Derinbay Ekici, Nermin Isik, Arif Ciloglu, Emrah Simsek, Gamze Yetismis and Abdullah Inci

Open Access

|Apr 2021

Figures & Tables

a: Cryptosporidium oocysts in fecal smears stained with the Ziehl-Neelsen by microscopic examination (×1000); b: Electrophoretic profiles of the SSU rRNA PCR products of C. parvum isolates digested with the endonucleases SspI (lanes 1 to 4) and MboII (lanes 5 to 8). M: Molecular size marker (100bp); c: Amplification curves with TaqMan real-time PCR analyses. x1, y1 and z1: C. parvum species specific qPCR curves; x2, y2 and z2: Cryptosporidium genus specific qPCR curves belonging to the same isolates; n: No DNA control and negative samples

Comparison of the results of microscopic examination and molecular assays in the detection of Cryptosporidium in calves and evaluation of diagnostic sensitivity and specificity_

Method		Molecular tools		Sensitivity	Specificity
Method		Positive	Negative	Sensitivity	Specificity
Microscopy	Positive	92	0	85.5%	100%
Microscopy	Negative	12	90	(80.7-93.9%)	(96.0-100.0%)
Total		104	90

Distribution and subtype of C_ parvum over age groups of the examined calves based on molecular diagnostic tests used in the study_

Age group (days)	Total no. of samples	Cryptosporidium parvum
Age group (days)	Total no. of samples	No. positive (%)	χ2	p	gp60 subtype
Early pre-weaned calves (≤ 15)	158	89 (56.3)	2.535	0.139	IIaA13G2R1
Pre-weaned calves (15-40)	36	15 (41.7)
Total	194	104 (53.6)

DOI: https://doi.org/10.2478/ebtj-2021-0010 | Journal eISSN: 2564-615X

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Language: English

Page range: 63 - 69

Published on: Apr 22, 2021

Published by: European Biotechnology Thematic Network Association

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

diarrheic calves,

nested PCR-RFLP,

Related subjects:

Bioinformatics,

Life sciences, other

© 2021 Alparslan Yildirim, Ferda Sevinc, Zuhal Onder, Onder Duzlu, Ozlem Derinbay Ekici, Nermin Isik, Arif Ciloglu, Emrah Simsek, Gamze Yetismis, Abdullah Inci, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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