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Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.) Cover

Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.)

The EuroBiotech Journal
Volume 5 (2021): Issue 1 (January 2021)
By: Gwenaëlle Deconninck and  Argyrios Gerakis  
Open Access
|Jan 2021

Figures & Tables

Figure 1

Effect of time and three disinfection-plus-scarification methods (NaClO, CENTRIFUGE, and PRESOAK) on the proportion of germinated vessels of Anacamptis laxiflora 189 d after sowing. Markers are measured points (n = 16) and lines are fitted models.
Effect of time and three disinfection-plus-scarification methods (NaClO, CENTRIFUGE, and PRESOAK) on the proportion of germinated vessels of Anacamptis laxiflora 189 d after sowing. Markers are measured points (n = 16) and lines are fitted models.

Figure 2

Germinated seeds of Anacamptis laxiflora 189 d after sowing.
Germinated seeds of Anacamptis laxiflora 189 d after sowing.

Figure 3

Effect of three disinfection-plus-scarification methods on the proportion of germinated vessels at 41 versus 189 d. At 189 d, the parameter estimates for the odds-ratios were not statistically significant for CENTRIFUGE (p>|t| = 0.720) and PRESOAK (p>|t| = 0.068) compared to the reference method, whereas at 41 d the parameter estimate was statistically significant for CENTRIFUGE (p>|t| < 0.001). Centrifugation seems to severely delay germination, although given enough time the proportion of germinated vessels catches up.
Effect of three disinfection-plus-scarification methods on the proportion of germinated vessels at 41 versus 189 d. At 189 d, the parameter estimates for the odds-ratios were not statistically significant for CENTRIFUGE (p>|t| = 0.720) and PRESOAK (p>|t| = 0.068) compared to the reference method, whereas at 41 d the parameter estimate was statistically significant for CENTRIFUGE (p>|t| < 0.001). Centrifugation seems to severely delay germination, although given enough time the proportion of germinated vessels catches up.

Figure 4

Inverse prediction: The curves predict disinfection/scarification time for a given germination rate, according to fitted model.
Inverse prediction: The curves predict disinfection/scarification time for a given germination rate, according to fitted model.

j_ebtj-2021-0004_tab_004_w2aab3b7c12b1b6b1ab1b2b2b6Aa

TermNEstimateSEzp > |z|OR95% C.I.
Intercept (β0)144-3.88660.447-8.694<.0010.021[0.008 – 0.047]
Duration (β1)4320.10230.00910.790<.0011.108[1.089 – 1.130]
Centrifuge (β2)1440.19690.3630.5430.5871.218[0.598 – 2.492]
Presoak (β3)1440.99470.3732.6640.0082.704[1.315 – 5.709]

Logistic regression results and parameter estimates for log odds-ratios from Eq_ [2]_ The regressors are duration of soaking in NaClO and scarification method_ The NaClO method was used as the reference

Model-LLDFχ2p > χ2
Difference142.213284.42<.001
Full145.08
Reduced287.29
R20.50
N432

Formula for modified “SM-organic” nutrient medium

IngredientsQuantity
Bottled water to make

Vikos® (Ioannina, Greece)

1 L
CaHPO499 mg

Equivalent to 75 mg Ca3(PO4)2 in the original formula

KH2PO475 mg
MgSO4.7H2O75 mg
Soluvit®

Fresenius Kabi, Uppsala, Sweden

10 mL
Amina-Fe

Humofert, Metamorfosi, Greece

0.92 mL

Equivalent to 0.5 mL Vaminolac® (Fresenius Kabi, Uppsala, Sweden) plus 10 mg FeSO4 in the original formula

Kinetin5 mg
Saccharose (sucrose)10 g
Activated charcoal1 g
Danish agar12 g
Pineapple juice25 mL
0.1 M H2SO4 for pH adjustment15–20 drops
Potato (Solanum tuber tuberosum)1 cm3 per culture vessel

Combinations of “dummy” or indicator variables that uniquely identify each scarification method for the purpose of logistic regression analysis_ The NaClO method is specified as reference, in which case X2 = X3 = 0

VariableNaClOCENTRIFUGEPRESOAK
X2010
X3001
DOI: https://doi.org/10.2478/ebtj-2021-0004 | Journal eISSN: 2564-615X
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Language: English
Page range: 15 - 23
Published on: Jan 22, 2021
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
centrifugation,
disinfection,
propagation,
Orchidaceae,
scarification
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2021 Gwenaëlle Deconninck, Argyrios Gerakis, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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