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Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.) Cover

Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.)

The EuroBiotech Journal
Volume 5 (2021): Issue 1 (January 2021)
By:  and    
Open Access
|Jan 2021
Figures & tables

Figures & Tables

Table 1

Formula for modified “SM-organic” nutrient medium

IngredientsQuantity
Bottled water to makea1 L
CaHPO499 mgb
KH2PO475 mg
MgSO4.7H2O75 mg
Soluvit®c10 mL
Amina-Fed0.92 mLe
Kinetin5 mg
Saccharose (sucrose)10 g
Activated charcoal1 g
Danish agar12 g
Pineapple juice25 mL
0.1 M H2SO4 for pH adjustment15–20 drops
Potato (Solanum tuber tuberosum)1 cm3 per culture vessel

a Vikos® (Ioannina, Greece)

b Equivalent to 75 mg Ca3(PO4)2 in the original formula

c Fresenius Kabi, Uppsala, Sweden

d Humofert, Metamorfosi, Greece

e Equivalent to 0.5 mL Vaminolac® (Fresenius Kabi, Uppsala, Sweden) plus 10 mg FeSO4 in the original formula

Table 2

Combinations of “dummy” or indicator variables that uniquely identify each scarification method for the purpose of logistic regression analysis. The NaClO method is specified as reference, in which case X2 = X3 = 0

VariableNaClOCENTRIFUGEPRESOAK
X2010
X3001
Figure 1

Effect of time and three disinfection-plus-scarification methods (NaClO, CENTRIFUGE, and PRESOAK) on the proportion of germinated vessels of Anacamptis laxiflora 189 d after sowing. Markers are measured points (n = 16) and lines are fitted models.

Figure 2

Germinated seeds of Anacamptis laxiflora 189 d after sowing.

Table 3

Logistic regression results and parameter estimates for log odds-ratios from Eq. [2]. The regressors are duration of soaking in NaClO and scarification method. The NaClO method was used as the reference

Model-LLDFχ2p > χ2
Difference142.213284.42<.001
Full145.08
Reduced287.29
R20.50
N432
TermNEstimateSEzp > |z|OR95% C.I.
Intercept (β0)144-3.88660.447-8.694<.0010.021[0.008 – 0.047]
Duration (β1)4320.10230.00910.790<.0011.108[1.089 – 1.130]
Centrifuge (β2)1440.19690.3630.5430.5871.218[0.598 – 2.492]
Presoak (β3)1440.99470.3732.6640.0082.704[1.315 – 5.709]

[i] LL = log likelihood; DF = degrees of freedom; χ2 = chi-square; p = probability; R2 = ratio of “Difference” to “Reduced” LL; N = observations; β0, β1, β2, β3 = fitted parameters; SE = standard error; z = estimate divided by its SE; OR = odds-ratio; C.I. = 95% confidence interval for OR.

Figure 3

Effect of three disinfection-plus-scarification methods on the proportion of germinated vessels at 41 versus 189 d. At 189 d, the parameter estimates for the odds-ratios were not statistically significant for CENTRIFUGE (p>|t| = 0.720) and PRESOAK (p>|t| = 0.068) compared to the reference method, whereas at 41 d the parameter estimate was statistically significant for CENTRIFUGE (p>|t| < 0.001). Centrifugation seems to severely delay germination, although given enough time the proportion of germinated vessels catches up.

Figure 4

Inverse prediction: The curves predict disinfection/scarification time for a given germination rate, according to fitted model.

DOI: https://doi.org/10.2478/ebtj-2021-0004 | Journal eISSN: 2564-615X
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Language: English
Page range: 15 - 23
Published on: Jan 22, 2021
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
centrifugation,
disinfection,
in vitro propagation,
Orchidaceae,
scarification
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2021 Gwenaëlle Deconninck, Argyrios Gerakis, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 5 (2021): Issue 1 (January 2021)
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