Figure 1
Results of different cryoprelyophilized mixture. 1) Freshly prepared mixture, 2) (0.5 % PEG 8000 + 2.0 % stachyose) Cryoprotectant mixture, 3) (0.5 % PEG 8000 + 1.0 % stachyose) Cryoprotectant mixture.
Figure 2
Results of lyophilized RT-PCR (a) and lyophilized LAMP (b) mixture. a: only 3. cryoprotectant mixture has positive result in RT-PCR assay. b: Results of LAMP assay 1) DNA ladder (100 bp, NEB), 2) 1. Cryoprotectant mixture, 3) 2. Cryoprotectant mixture, 4) 3. Cryoprotectant mixture 5) Freshly prepared mixture.
Figure 3
RT-PCR results of thermal stability of lyophilized mixtures
Figure 4
Detection limit results of lyophilized RT-PCR (a) and LAMP (b). a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control. b: a: 1) 105 CFU/ml, 2) 104 CFU/ml, 3) 103 CFU/ml, 4) 102 CFU/ml, 5) 101 CFU/ml, 6) negative control
Figure 5
An image of lyophilized mixture in PCR tubes.