Have a personal or library account? Click to login
Revealing the therapeutic effects of aminolevulinate mediated femtosecond laser induced photo-chemotherapy in different cancer cells Cover

Revealing the therapeutic effects of aminolevulinate mediated femtosecond laser induced photo-chemotherapy in different cancer cells

The EuroBiotech Journal
Volume 4 (2020): Issue 4 (October 2020)
By: Meltem Demirel Kars,  Gamze Yıldırım,  Yasemin Gündoğdu,  Fatmanur Gönce,  Esra Ayan and  Hamdi Şükür Kılıç  
Open Access
|Oct 2020

Figures & Tables

Figure 1

Cancer model cell lines: A) UMUC-3, B) SK-MEL30 C) MCF-7 (Invert, phase contrast light microscope)
Cancer model cell lines: A) UMUC-3, B) SK-MEL30 C) MCF-7 (Invert, phase contrast light microscope)

Figure 2

Cell viability profiles of the A) UMUC-3 cells, B) SK-MEL-30, C) MCF-7 cells at the selected PDT wavelength conditions. The significantly different groups were demonstrated by asterisk (p<0.05).
Cell viability profiles of the A) UMUC-3 cells, B) SK-MEL-30, C) MCF-7 cells at the selected PDT wavelength conditions. The significantly different groups were demonstrated by asterisk (p<0.05).

Figure 3

Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated UMUC-3 cells.
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated UMUC-3 cells.

Figure 4

Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated SK-MEL-30 cells.
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated SK-MEL-30 cells.

Figure 5

Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated MCF-7 cells.
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated MCF-7 cells.

Figure 6

Intracellular fluorescent (PpIX) accumulation induced by ALA treatment observed after PBS washes, (40X-oil, 512x512). A) UMUC-3 cells non-ALA treated, observed by confocal microscope with standard detector-SD; B) UMUC-3 cells non-ALA treated, observed in white field by transmitted detector-TD; C) ALA treated UMUC-3 cells, observed by confocal microscope with SD; D) ALA treated UMUC-3 cells observed by white field and confocal with SD and TD; E) SK-MEL-30 cells non-ALA treated, observed by confocal microscope with SD; F) SK-MEL-30 cells non-ALA treated, observed in white field by TD; G) ALA treated SK-MEL-30 cells, observed by confocal microscope with SD; H) ALA treated SK-MEL-30 cells observed by white field and confocal with SD and TD; I) MCF-7 cells non-ALA treated, observed by confocal microscope with SD; J) MCF-7 cells non-ALA treated, observed in white field by TD; K) ALA treated MCF-7 cells, observed by confocal microscope with SD; L) ALA treated MCF-7 cells, observed by white field and confocal with SD and TD. (Excitation: 405 nm; emission: 614 nm)
Intracellular fluorescent (PpIX) accumulation induced by ALA treatment observed after PBS washes, (40X-oil, 512x512). A) UMUC-3 cells non-ALA treated, observed by confocal microscope with standard detector-SD; B) UMUC-3 cells non-ALA treated, observed in white field by transmitted detector-TD; C) ALA treated UMUC-3 cells, observed by confocal microscope with SD; D) ALA treated UMUC-3 cells observed by white field and confocal with SD and TD; E) SK-MEL-30 cells non-ALA treated, observed by confocal microscope with SD; F) SK-MEL-30 cells non-ALA treated, observed in white field by TD; G) ALA treated SK-MEL-30 cells, observed by confocal microscope with SD; H) ALA treated SK-MEL-30 cells observed by white field and confocal with SD and TD; I) MCF-7 cells non-ALA treated, observed by confocal microscope with SD; J) MCF-7 cells non-ALA treated, observed in white field by TD; K) ALA treated MCF-7 cells, observed by confocal microscope with SD; L) ALA treated MCF-7 cells, observed by white field and confocal with SD and TD. (Excitation: 405 nm; emission: 614 nm)

Representation of % cell populations that are living and that experience cell death through apoptosis and necrosis after PDT_ (* Considerable cell death percentages)

DOI: https://doi.org/10.2478/ebtj-2020-0024 | Journal eISSN: 2564-615X
Journal RSS Feed
Language: English
Page range: 207 - 215
Published on: Oct 21, 2020
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Aminolevulinate,
apoptosis,
MCF-7,
Photodynamic therapy,
SK-MEL-30,
UMUC-3
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2020 Meltem Demirel Kars, Gamze Yıldırım, Yasemin Gündoğdu, Fatmanur Gönce, Esra Ayan, Hamdi Şükür Kılıç, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 4 (2020): Issue 4 (October 2020)Next article