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Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa Cover

Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa

The EuroBiotech Journal
Volume 4 (2020): Issue 3 (July 2020)
By: David C. Nwobodo,  Chibueze P. Ihekwereme and  Festus B. C. Okoye  
Open Access
|Jul 2020

Figures & Tables

Figure 1

Macroscopic morphologies of the pure endophytic isolates.(Cn1 - Acremonium sp., Cn2 - Aspergillus sp., Cn3 - Trichophyton sp.)
Macroscopic morphologies of the pure endophytic isolates.(Cn1 - Acremonium sp., Cn2 - Aspergillus sp., Cn3 - Trichophyton sp.)

Figure 2

a: HPLC chromatogram of Cn3 extract showing protocatechuic acid; its UV spectrum and structure.b: HPLC chromatogram of Cn3 extract showing p-hydroxybenzoic acid; its U V spectrum and structure.
a: HPLC chromatogram of Cn3 extract showing protocatechuic acid; its UV spectrum and structure.b: HPLC chromatogram of Cn3 extract showing p-hydroxybenzoic acid; its U V spectrum and structure.

Figure 3

Antipseudomonal zones of inhibition of the fungal extracts against various clinical isolates (IZD in mm). Values represent the mean of experiments in triplicates.Ps.a A = Laboratory isolate, Ps.a B=Orthopedic wound infection, Ps.a C= Urine, Ps.a D=Sputum isolate, Ps.a E = Vaginal swab Isolate.
Antipseudomonal zones of inhibition of the fungal extracts against various clinical isolates (IZD in mm). Values represent the mean of experiments in triplicates.Ps.a A = Laboratory isolate, Ps.a B=Orthopedic wound infection, Ps.a C= Urine, Ps.a D=Sputum isolate, Ps.a E = Vaginal swab Isolate.

The antimicrobial activities of compounds detected in the fungal extracts

Fungi ExtractIdentified CompoundReported Biological Activities
Cn14-hydroxyphenyl acetic acidAntimicrobial (18, 19)
Indole-3-acetic acidAntimicrobial (20, 21)
Cn2P-hydroxybenzoic acidAntimicrobial (22, 23)
Cn3Protocatechuic acidAntimicrobial (23, 24, 25, 26)
P-hydroxybenzoic acidAntimicrobial (22, 23)

Cultural morphology and microscopic features of the endophytic fungi isolates from C_ nitida

IsolatesFormElevationMarginColourReverseGrowth RateTextureHyphaeSporesDiameter (mm)Suspected Organism
Cn1CircularFlatCurledOrangeLight orangeSlow/moderateSlimy and moistSeptedMicro conidia75Acremonium (15)
Cn2CircularRaisedlobateWhiteColourless with stripesModerateThick cottony and DrySeptedMicro conidia88Aspergillus II (16)
Cn3CircularNmbonate/FlatEntirewhiteAbsent or pale brown-ish-yellow with ageModerateCottony SmoothSeptedMicro conidia60Trichophyton (17)
DOI: https://doi.org/10.2478/ebtj-2020-0019 | Journal eISSN: 2564-615X
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Language: English
Page range: 161 - 166
Published on: Jul 19, 2020
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Endophytic fungi,
antimicrobial activity,
secondary metabolites
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2020 David C. Nwobodo, Chibueze P. Ihekwereme, Festus B. C. Okoye, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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