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The role of C-terminal amidation in the mechanism of action of the antimicrobial peptide aurein 1.2 Cover

The role of C-terminal amidation in the mechanism of action of the antimicrobial peptide aurein 1.2

The EuroBiotech Journal
Volume 4 (2020): Issue 1 (January 2020)
By: Mahdi Shahmiri and  Adam Mechler  
Open Access
|Jan 2020

Figures & Tables

Figure 1

ΔD vs Δf plots of aurein1.2-NH-CH3 interaction with DMPC and DMPC:cholesterol (9:1) membranes at different concentrations. The effect is shown for the seventh harmonic of the fundamental frequency of the sensor chip.

Figure 2

ΔD vs Δf plots of aurein1.2-NH-CH3 interaction with DMPC/DMPG (4:1) and DMPC/DMPG (3:2) at different concentrations. The effect is shown for the seventh harmonic of the fundamental frequency of the sensor chip.

Figure 3

Comparison of the viscoelastic fingerprints of aurein1.2-NH-CH3 (red line) and aurein1.2-NH2 (blue line). The effect is shown for the seventh harmonic of the fundamental frequency of the sensor chip.

Figure 4

Comparison of the viscoelastic fingerprints of aurein1.2-NH-CH3 (red line) and aurein1.2-NH2 (blue line). The effect is shown for the seventh harmonic of the fundamental frequency of the sensor chip.

Figure 5

Comparison of the space filling models of the C-terminal region. The last residue (F13) is shown in full; the rest of the peptide is represented with an R group (large sphere in the figure). A) unmodified carboxyl terminus; B) primary amide (wild type) terminus; C) secondary amide with methyl terminus.

Dye leakage results with neat DMPC liposomes

PeptidesFluorescence intensity increase (%)
Aurein 1.2 –NH2 (wild type)30
Aurein 1.2 –COOH1
Aurein 1.2 –NH-CH30

Results of CD measurements

Medium%Helicity
aurein1.2-NH2aurein1.2-NH-CH3aurein1.2-COOH
PBS949
DMPC25524
DMPC:cholesterol 9:120512
DMPC:DMPG 4:138832
DOI: https://doi.org/10.2478/ebtj-2020-0004 | Journal eISSN: 2564-615X
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Language: English
Page range: 25 - 31
Published on: Jan 18, 2020
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
antimicrobial peptide,
membrane disruption,
carpet mechanism,
activity
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2020 Mahdi Shahmiri, Adam Mechler, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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