Table 1
GC-MS analysis of essential oil components from A. millefolium. According to Peak report 10 different molecule were identified in essential oil. Four of these molecules are significantly higher than other molecules.
| Peak Report Total Ion Chromatogram | ||||
|---|---|---|---|---|
| Peak # | Molecule | R. Time | Area | Area % ↓ |
| 3 | 1,8-Cineole | 8.127 | 83328771 | 27.34 |
| 4 | Camphor (CAS) | 9.585 | 74017016 | 24.28 |
| 10 | .beta.-Eudesmol (CAS) | 14.158 | 57129364 | 18.74 |
| 8 | Caryophyllene oxide | 13.595 | 25349728 | 8.32 |
| 5 | Bicyclo[2.2.1]heptan-2-ol. 1.7.7-trimethyl-, (1S-endo)- | 9.832 | 16313677 | 5.35 |
| 1 | Camphene | 7.053 | 10954991 | 3.59 |
| 9 | Viridiflorol | 13.681 | 10656458 | 3.50 |
| 2 | Bicyclo[3.1.1]heptan. 6.6-dimethyl-2-methylene-. (1S)- | 7.424 | 9919490 | 3.25 |
| 7 | .alpha.-Terpineol | 10.005 | 8602416 | 2.82 |
| 6 | 3-Cyclohexen-1-ol. 4-methyl-1-(1-methylethyl)-. (R)- | 9.880 | 8528233 | 2.80 |
| 304800144 | 100.00 | |||
Figure 1
Ingenuity Pathway Analysis software for the network analysis for the most abundant four molecules (1,8-Cineole, Camphor, β-Eudesmol and Camphene). Compound related networks were depicted in A, B, C and D, respectively.
Figure 2
Annexin V / 7-AAD test results total apoptosis rates for HeLa cell line. In comparison with DMSO control group essential oil shows apoptotic effects on cancer cell line.
Figure 3
Yarrow essential oils treatment results as Dot-Plot on HeLa cell line. Number of healthy cells were decreased after essential oil treatment and apoptotic and dead cell percentage were increased.
Figure 4
Annexin V / 7-AAD test and live cell percentage of HeLa cells. Number of living cells were decreased significantly when compared DMSO control group.
Figure 5
Cell cycle test for HeLa cell line. Essential oil application blocks the cell cycle in G0/G1 phase when compared DMSO control group.
Figure 6
Ki-67 test and proliferating cell (+) ratios for HeLa cell line. Ki-67 proliferation test shows in correlation with cell cycle tests essential oil application inhibits the proliferation of cervix cancer cells in all concentration.
Figure 7
Proliferating cell percentage as Volcano Plot graph of HeLa cells in Control, DMSO Control and ACH groups. According to Ki-67 Proliferation Assay results proliferation of cells blocked by essential oil of A. millefolium.