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Genetic bases of tomatо marker-assisted selection in Belarus Cover

Genetic bases of tomatо marker-assisted selection in Belarus

The EuroBiotech Journal
Volume 2 (2018): Issue 2 (April 2018)
By: Olga G. Babak,  Natalia A. Nekrashevich,  Konstantsia K. Yatsevich,  Sergey V. Malyshev and  Alexander V. Kilchevsky  
Open Access
|Apr 2018

Figures & Tables

Figure 1

PCR amplification results with tF/ tR SCAR marker. Lines: 1,2 - LA3134, 3,4 - LA2529, 5,6,7 - LA2374, 8, 9,10- LA3899, 11,12,13 - LA4062, 14,15,16 - LA3183, 17,18,19 - LA348.
PCR amplification results with tF/ tR SCAR marker. Lines: 1,2 - LA3134, 3,4 - LA2529, 5,6,7 - LA2374, 8, 9,10- LA3899, 11,12,13 - LA4062, 14,15,16 - LA3183, 17,18,19 - LA348.

Figure 2

PCR amplification results with OgcF/ OgcR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 9-10 – with Ogc allele, B - fragment analysis on an automatic sequencer: homo- and heterozygous samples.
PCR amplification results with OgcF/ OgcR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 9-10 – with Ogc allele, B - fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 3

PCR amplification results with BpromF/BpromR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 7-8 – with b allele, 3-4, 5-6 – with B allele B – fragment analysis on an automatic sequencer: homo- and heterozygous samples.
PCR amplification results with BpromF/BpromR SCAR marker. А - Polyacrylamide gel. Lines: 1-2, 7-8 – with b allele, 3-4, 5-6 – with B allele B – fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 4

PCR amplification results with the SCAR marker RinF1/ RinwR1/RinmR2. Lines: 1-3 – L154, 4,5 – Mо 577 (rin), 6 -8 –F1 hybrid (L154 х Мо 577).
PCR amplification results with the SCAR marker RinF1/ RinwR1/RinmR2. Lines: 1-3 – L154, 4,5 – Mо 577 (rin), 6 -8 –F1 hybrid (L154 х Мо 577).

Figure 5

Results of PCR amplification of DNA tomato forms with NorF/ NorR SCAR marker: 1, 2 –Mo 948 (nor), 3, 4 – Мо 950 (norA), 5, 6 – Line 19/1 (norwt ) (А); Results of fragment analysis of plants F2: homozygous plant (nor/nor) (B) and heterozygous plant (nor/norwt) (C).
Results of PCR amplification of DNA tomato forms with NorF/ NorR SCAR marker: 1, 2 –Mo 948 (nor), 3, 4 – Мо 950 (norA), 5, 6 – Line 19/1 (norwt ) (А); Results of fragment analysis of plants F2: homozygous plant (nor/nor) (B) and heterozygous plant (nor/norwt) (C).

Figure 6

A fragment of the DNA-sequence of the second exon of the LeNAC-NOR gene in the norA mutant and the wild allele. The arrow shows the position of the point mutation in the norA allele.t (nor/norwt) (C).
A fragment of the DNA-sequence of the second exon of the LeNAC-NOR gene in the norA mutant and the wild allele. The arrow shows the position of the point mutation in the norA allele.t (nor/norwt) (C).

Figure 7

Restriction products of the CAPS marker (dAlc-BspMII) used for norA allele identification. Lines:1 – La 0059 (norwt), 2 – Mo 948 (nor), 3 –Mo 950 (norA), 4 – La2529 (norA), 5 – La3134 (norA), 6 –Mo 577 (rin), 7 – F1norA/norwt.
Restriction products of the CAPS marker (dAlc-BspMII) used for norA allele identification. Lines:1 – La 0059 (norwt), 2 – Mo 948 (nor), 3 –Mo 950 (norA), 4 – La2529 (norA), 5 – La3134 (norA), 6 –Mo 577 (rin), 7 – F1norA/norwt.

Figure 8

The results of DNA amplification of tomato genotypes with the gf-3F/gf-3R marker: А. Polyacrylamide gel. Lines: 1, 2 – Black Prince (gf-3), 3, 4 – Black cherry, 5, 6 – Noire charbonneuse; B. Fragment analysis on an automatic sequencer: homo- and heterozygous samples.
The results of DNA amplification of tomato genotypes with the gf-3F/gf-3R marker: А. Polyacrylamide gel. Lines: 1, 2 – Black Prince (gf-3), 3, 4 – Black cherry, 5, 6 – Noire charbonneuse; B. Fragment analysis on an automatic sequencer: homo- and heterozygous samples.

Figure 9

The results of DNA amplification of tomato in F3 population genotypes with hp-2dg F/hp-2dg R and subsequent restriction by AclI. № 1-10, 12 – homozygous hp-2dg form, No. 14, 16, 17 – heterozygous form, No. 11, 13, 18 – samples with wild allele.
The results of DNA amplification of tomato in F3 population genotypes with hp-2dg F/hp-2dg R and subsequent restriction by AclI. № 1-10, 12 – homozygous hp-2dg form, No. 14, 16, 17 – heterozygous form, No. 11, 13, 18 – samples with wild allele.

Figure 10

Forms of tomato with various structural and regulatory genes: ogc/hp2dg, B/hp2dg, B/gf-3, B/hp2dg/nor, ogc/gf-3/rin.
Forms of tomato with various structural and regulatory genes: ogc/hp2dg, B/hp2dg, B/gf-3, B/hp2dg/nor, ogc/gf-3/rin.

Figure 11

Peculiarities of the carotenoid accumulation in tomato forms with ogc allele. Genotypes of the analysed tomato forms: 1 – ogc/ogc//rinwt/rinwt, 2 – ogc/ogc//rin/rin, 3 – ogc/ogc//gf-3/gf-3//rinwt/rinwt, 4 – ogc/ogc//gf-3/gf-3//rin/rin, 5 – ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt, 6 – ogc/ogc//hp2dg/hp2dg//rin/rin, 7 – ogc/ogc/nor wt/norwt, 8 – ogc/ogc/nor /nor, 9 – ogc/ogc//gf-3/gf-3//nor/nor, 10 – ogc/ogc//hp2dg/hp2 dg//norwt/norwt, 11 – ogc/ogc//hp2dg/hp2 dg//nor/nor.
Peculiarities of the carotenoid accumulation in tomato forms with ogc allele. Genotypes of the analysed tomato forms: 1 – ogc/ogc//rinwt/rinwt, 2 – ogc/ogc//rin/rin, 3 – ogc/ogc//gf-3/gf-3//rinwt/rinwt, 4 – ogc/ogc//gf-3/gf-3//rin/rin, 5 – ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt, 6 – ogc/ogc//hp2dg/hp2dg//rin/rin, 7 – ogc/ogc/nor wt/norwt, 8 – ogc/ogc/nor /nor, 9 – ogc/ogc//gf-3/gf-3//nor/nor, 10 – ogc/ogc//hp2dg/hp2 dg//norwt/norwt, 11 – ogc/ogc//hp2dg/hp2 dg//nor/nor.

Figure 12

Peculiarities of the carotenoid accumulation in tomato forms with B allele. Genotypes of the analysed tomato forms: 1 – B/B//rinwt/rinwt, 2 – B/B//rin/rin, 3 – B/B//gf-3/gf-3//rinwt/rinwt, 4 – B/B//gf-3/gf-3//rin/rin, 5 – B/B//hp2dg/hp2dg//rinwt/rinwt, 6 – B/B//hp2dg/hp2dg//rin/rin, 7 – B/B//norwt/norwt, 8 – B/B//nor/nor, 9 – B/B//gf-3/gf-3//nor/nor, 10 – B/B//hp2dg/hp2dg//norwt/norwtt, 11 – B/B//hp2dg/hp2dg//nor/nor.
Peculiarities of the carotenoid accumulation in tomato forms with B allele. Genotypes of the analysed tomato forms: 1 – B/B//rinwt/rinwt, 2 – B/B//rin/rin, 3 – B/B//gf-3/gf-3//rinwt/rinwt, 4 – B/B//gf-3/gf-3//rin/rin, 5 – B/B//hp2dg/hp2dg//rinwt/rinwt, 6 – B/B//hp2dg/hp2dg//rin/rin, 7 – B/B//norwt/norwt, 8 – B/B//nor/nor, 9 – B/B//gf-3/gf-3//nor/nor, 10 – B/B//hp2dg/hp2dg//norwt/norwtt, 11 – B/B//hp2dg/hp2dg//nor/nor.

Carotenoid content in tomato samples with tangerine and non-ripening alleles (mg/100 g wet basis), 2016

Allele combinationsneurosporinζ - carotene
t/t//norwt/norwt11.60
t/t//norA/norA07.2
t/t//nor/nor1.940.94

The content of carotenoids in tomato samples with different combinations of quality alleles (mg/100 g wet basis), 2016

Allele combinationsβ- caroteneLycopeneLuteinChlorophyll
ab
ogc/ogc//rinwt/rinwt0.8511.0000
ogc/ogc//rin/rin0.4400.2600
ogc/ogc//gf-3/gf-3//rinwt/rinwt0.555.75000
ogc/ogc//gf-3/gf-3//rin/rin0,250.8000
ogc/ogc//hp2dg/hp2 dg//rinwt/rinwt1.0516.92.200
ogc/ogc//hp2dg/hp2 dg//rin/rin0.701.10.600.62
ogc/ogc//norwt/norwt1.158.7000
ogc/ogc//nor/nor0.71.9000
ogc/ogc//gf-3/gf-3//nor/nor0.833.40.570.280.28
ogc/ogc//hp2dg/hp2dg//norwt/norwt1.719.92.200
ogc/ogc//hp2dg/hp2dg//nor/nor1.40.71.400
B/B//rinwt/rinwt2.91.01.200
B/B//rin/rin0.6900.600
B/B//gf-3/gf-3//rinwt/rinwt3.12.1000
B/B//gf-3/gf-3//rin/rin0.600.250.870.93
B/B//hp2dg/hp2 dg//rinwt/rinwt5.502.1500
B/B//hp2dg/hp2 dg//rin/rin0.600.70.680.63
B/B//norwt/norwt4.71.430.7500
B/B//gf-3/gf-3//norwt/norwt1.85.51.03.02.1
B/B//gf-3/gf-3//nor/nor0.28002.32.6
B/B//hp2dg/hp2 dg//norwt/norwt5.101.000
B/B//hp2dg/hp2 dg//nor/nor1.630.31.21.010.80
DOI: https://doi.org/10.2478/ebtj-2018-0017 | Journal eISSN: 2564-615X
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Language: English
Page range: 128 - 135
Published on: Apr 25, 2018
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
tomato,
DNA-marker,
fruit quality breeding
Related subjects:
Life sciences,
Life sciences, other,
Medicine,
Biomedical engineering,
Physics,
Nanotechnology,
Biophysics

© 2018 Olga G. Babak, Natalia A. Nekrashevich, Konstantsia K. Yatsevich, Sergey V. Malyshev, Alexander V. Kilchevsky, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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