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Main products of biotransformation of (1-14C)-Trinitrotoluene in culture liquid
| Strain | Radioactivity, in% | |
|---|---|---|
| Organic acids | Amino acids | |
| Mucor spT1-1 | 72.2 | 27.8 |
| Trichoderma sp N2-6 | 77.4 | 22.6 |
| Aspergillus niger N2-2 | 89.8 | 10.2 |
Changes of CFU of introduced and indigenous fungi in TNT contaminated soils under natural modeling conditions
| Test variant | CFU of fungi per 1g of soil | ||
|---|---|---|---|
| At the moment of inoculation | 40 days later | 100 days later | |
| Red soil (sterile soil) + TNT | 0 | 0 | 0 |
| Red soil (nonsterile soil) + TNT | 3.8×106 | 1.2×106 | 1.3×105 |
| Red soil (nonterile soil) + TNT + Aspergillus niger N2-2 | 5.4×106 | 3.9 ×105 | 4.9×106 |
| Red soil (nonsterile soil) + TNT + Mucor sp. T1-1 | 1.9×106 | 7.2×105 | 1.3×106 |
| Black soil (sterile soil) + TNT | 0 | 0 | 0 |
| Black soil (nonsterile soil) + TNT | 6.5×106 | 4.7×105 | 3.2×105 |
| Black soil (nonsterile soil) + TNT + Aspergillus niger N2-2 | 5×106 | 8.8×105 | 5.7×106 |
| Black soil (nonsterile soil) + TNT + Mucor sp. T1-1 | 1.9×106 | 7.0×105 | 2.4×106 |
The amount of (1-14C)-TNT detected in fungi biomass, in%
| Culture | Nutrient medium #2 | Biomass, mg | Total radioactivity of biomass, in % |
|---|---|---|---|
| Mucor sp. T1-1 | Czapek’s medium | 29 | 53.6 |
| Trichoderma sp N2-6 | Czapek’s medium | 25 | 51.9 |
| Aspergillus niger N2-2 | Czapek’s medium | 27 | 52.9 |
Changes of CFU of fungi introduced into TNT-contaminated soils
| Strain | Soil types | CFU per g of soil | |||
|---|---|---|---|---|---|
| Initial amount | 10 days later | 20 days later | 30 days later | ||
| Mucor sp. Tl-1 | Black soil | 7,5x105 | 9,5x106 | 6,5x105 | 3,5x104 |
| Red soil | 7,5x105 | 5,5x105 | 3,5x104 | 2,3x104 | |
| Aspergillus niger N2-2 | Black soil | 8,5x106 | 4,5x107 | 5,5x105 | 6,5x104 |
| Red soil | 8,5.106 | 4,5x106 | 3,5x105 | 4,5x104 | |
Radioactivity of amino acids products of (1-14С) TNT transformation by fungi strains
| Strain | Radioactivity of amino acids in % | Distribution of radioactivity in individual amino acids, in % | |||||
|---|---|---|---|---|---|---|---|
| Phenylalanine | Glutamic acid | Tyrosine | Arginine | Asparaginic acid | Serine | ||
| Mucor sp.T1-1 | 27.8 | 27.4 | 19.3 | 17.5 | 13.6 | 11.5 | 10.7 |
| Trichoderma sp. N2-6 | 22.6 | 24.3 | 16.6 | 18.7 | 18.5 | 11.5 | 10.4 |
| Aspergillus niger N2-2 | 10.2 | 38.9 | 14.7 | 12.8 | 11.4 | 11.8 | 10.4 |
Distribution of radioactivity of (1-14С)-trinitrotoluene transformation by fungi strains among organic acids
| Strain | Total radioactivity in %, of low molecular compounds fraction | Distribution of radioactivity in organic acids, in % | |||||
|---|---|---|---|---|---|---|---|
| Fumaric acid | Succinic acid | Glycolic acid | Citric acid | Malic acid | Unknown | ||
| Mucor sp.T1-1 | 72.0 | 86.6 | 4.1 | 2.8 | 2.7 | 1.8 | 2.0 |
| Trichoderma sp. N2-6 | 77.4 | 91.8 | 4.0 | 1.5 | 1.1 | 0.8 | 0.8 |
| Aspergillus niger N2-2 | 89.8 | 88.8 | 3.3 | 3.7 | 1.2 | 0.7 | 2.3 |