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Characteristics of the studies for the association of IL1A rs17561, IFN-γ rs2430561, STOX1 rs1341667, and PPAR-γ rs1801282 gene polymorphism with PE
| Gene | Study | Ethnicity | Country | PE Cases | Controls | HWE P-value | HWE adjusted P-value | NOS Score | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AC_Cases | CC_Cases | AA_Cases | AC_Controls | CC_Controls | AA_Controls | |||||||
| IL1A rs (17561) | Li et al., 2014[5] | Asian | China | 53 | 344 | 5 | 111 | 436 | 7 | 0 | 0 | 8 |
| Andraweera et al., 2016[10] | Asian | Sri Lanka | 77 | 79 | 16 | 92 | 60 | 16 | 0.1865 | 0.1865 | 7 | |
| Wu et al., 2017[11] | Asian | China | 15 | 76 | 0 | 39 | 192 | 1 | 0 | 0 | 7 | |
| TT_Cases | TA_Cases | AA_Cases | TT_Controls | TA_Controls | AA_Controls | |||||||
| IFN-γ (rs2430561) | Kamali-Sarvestani et al., 2006[12] | Asian | Iran | 21 | 66 | 42 | 30 | 78 | 53 | 0.8903 | 0.8903 | 6 |
| Daher et al., 2006[13] | Brazil | 6 | 25 | 24 | 22 | 38 | 29 | 0.1842 | 0.8776 | 7 | ||
| Daher et al., 2006[13] | Brazil | 9 | 52 | 34 | 17 | 43 | 36 | 0.5075 | 0.8776 | 8 | ||
| De Lima et al., 2009[14] | Caucasian | Brazil | 7 | 46 | 32 | 18 | 45 | 33 | 0.7021 | 0.8776 | 8 | |
| Pinheiro et al., 2015[15] | Caucasian | Brazil | 33 | 51 | 32 | 5 | 40 | 62 | 0.6497 | 0.8776 | 7 | |
| TT_Cases | TC_Cases | CC_Cases | TT_Controls | TC_Controls | CC_Controls | |||||||
| STOX1 (rs1341667) | Kim et al., 2009[18] | Asian | Korea | 155 | 42 | 5 | 150 | 53 | 1 | 0.1041 | 0.4164 | 7 |
| Fenstad et al., 2010[19] | Caucasian | Norway | 124 | 450 | 412 | 245 | 931 | 840 | 0.5981 | 0.9707 | 7 | |
| Pinarbasi et al., 2020[20] | Asian | Turkey | 108 | 280 | 112 | 143 | 249 | 108 | 0.9839 | 0.9839 | 7 | |
| Bildirici et al., 2023[17] | Asian | Turkey | 10 | 31 | 9 | 18 | 23 | 9 | 0.728 | 0.9707 | 8 | |
| CC_Cases | CG_Cases | GG_Cases | CC_Controls | CG_Controls | GG_Controls | |||||||
| PPAR-γ (rs1801282) | Laasanen et al., 2002[16] | Caucasian | Finland | 95 | 36 | 2 | 76 | 34 | 5 | 0.6334 | 0.6334 | 8 |
| Ghorbani et al., 2021[21] | Asian | Iran | 75 | 25 | 0 | 77 | 23 | 0 | 0.1938 | 0.5814 | 6 | |
| Liu et al., 2021[9] | Caucasian | France | 26 | 8 | 1 | 1297 | 301 | 15 | 0.5922 | 0.6334 | 8 | |
Subgroup analysis for association of PPAR-γ rs1801282 gene polymorphism with PE_
| Model | Ethnicity | Number of studies | OR | Test of association 95% Cl | p-value | Model | Test of heterogeneity p-value | I2 |
|---|---|---|---|---|---|---|---|---|
| Allele contrast (A vs. a) | Overall | 3 | 1.0252 | [0.7404; 1.4197] | 0.880652 | Fixed | 0.2569 | 0.2642 |
| Asian | 1 | 0.9096 | [0.4974; 1.6634] | 0.758349 | Fixed | NA | NA | |
| Caucasian | 2 | 1.0768 | [0.7316; 1.5849] | 0.707445 | Fixed | 0.1135 | 0.6008 | |
| Recessive model (AA vs. Aa+aa) | Overall | 3 | 1.0002 | [0.6944; 1.4408] | 0.999026 | Fixed | 0.4203 | 0 |
| Asian | 1 | 0.8961 | [0.4681; 1.7156] | 0.740601 | Fixed | NA | NA | |
| Caucasian | 2 | 1.0523 | [0.6769; 1.6358] | 0.820909 | Fixed | 0.2098 | 0.3641 | |
| Dominant model (AA+Aa vs. aa) | Overall | 2 | 1.0613 | [0.1197; 9.4081] | 0.957354 | Random | 0.0973 | 0.6364 |
| Asian | 0 | NA | Fixed | NA | NA | |||
| Caucasian | 2 | 1.0613 | [0.1197; 9.4081] | 0.957354 | Random | 0.0973 | 0.6364 | |
| Overdominant (Aa vs. AA + aa) | Overall | 3 | 1.0367 | [0.7141; 1.5050] | 0.849601 | Fixed | 0.7199 | 0 |
| Asian | 1 | 1.1159 | [0.5829; 2.1365] | 0.740601 | Fixed | NA | NA | |
| Caucasian | 2 | 0.9999 | [0.6343; 1.5762] | 0.999641 | Fixed | 0.4449 | 0 | |
| pairwl (AA vs. aa) | Overall | 2 | 1.0518 | [0.1067; 10.3726] | 0.965492 | Random | 0.0835 | 0.6662 |
| Asian | 0 | NA | Fixed | NA | NA | |||
| Caucasian | 2 | 1.0518 | [0.1067; 10.3726] | 0.965492 | Random | 0.0835 | 0.6662 | |
| pairw2 (AA vs. Aa) | Overall | 3 | 0.9773 | [0.6723; 1.4208] | 0.904274 | Fixed | 0.6343 | 0 |
| Asian | 1 | 0.8961 | [0.4681; 1.7156] | 0.740601 | Fixed | NA | NA | |
| Caucasian | 2 | 1.0203 | [0.6456; 1.6127] | 0.931304 | Fixed | 0.3687 | 0 | |
| pairw3 (Aa vs. aa) | Overall | 2 | 1.2702 | [0.3345; 4.8237] | 0.725342 | Fixed | 0.1755 | 0.4553 |
| Asian | 0 | NA | Fixed | NA | NA | |||
| Caucasian | 2 | 1.2702 | [0.3345; 4.8237] | 0.725342 | Fixed | 0.1755 | 0.4553 |
Subgroup analysis for the association of STOX1 rs1341667 gene polymorphism with PE_
| Model | Ethnicity | Number of studies | OR | Test of association 95% CI | p-value | Model | Test of heterogeneity p-value | I2 |
|---|---|---|---|---|---|---|---|---|
| Allele contrast (A vs. a) | Overall | 4 | 0.9573 | [0.8739; 1.0486] | 0.347607 | Fixed | 0.3168 | 0.1503 |
| Asian | 3 | 0.8704 | [0.7456; 1.0162] | 0.079072 | Fixed | 0.5181 | 0 | |
| Caucasian | 1 | 1.0067 | [0.8994; 1.1268] | 0.907561 | Fixed | NA | NA | |
| Recessive model (AA vs. Aa+aa) | Overall | 4 | 0.8644 | [0.6250; 1.1955] | 0.378488 | Random | 0.0343 | 0.6532 |
| Asian | 3 | 0.7751 | [0.4843; 1.2405] | 0.288338 | Random | 0.0621 | 0.6401 | |
| Caucasian | 1 | 1.0398 | [0.8255; 1.3098] | 0.740104 | Fixed | NA | NA | |
| Dominant model (AA+Aa vs. aa) | Overall | 4 | 0.9803 | [0.8559; 1.1229] | 0.774395 | Fixed | 0.5249 | 0 |
| Asian | 3 | 0.9317 | [0.7009; 1.2385] | 0.626105 | Fixed | 0.354 | 0.0371 | |
| Caucasian | 1 | 0.9951 | [0.8527; 1.1614] | 0.950762 | Fixed | NA | NA | |
| Overdominant (Aa vs. AA+aa) | Overall | 4 | 1.0762 | [0.8331; 1.3902] | 0.574082 | Random | 0.0561 | 0.603 |
| Asian | 3 | 1.1554 | [0.7391; 1.8061] | 0.526314 | Random | 0.0609 | 0.6426 | |
| Caucasian | 1 | 0.9784 | [0.8397; 1.1400] | 0.779761 | Fixed | NA | NA | |
| pairwise 1 (AA vs. aa) | Overall | 4 | 0.9008 | [0.7371; 1.0998] | 0.303905 | Fixed | 0.1801 | 0.3863 |
| Asian | 3 | 0.69 | [0.4893; 0.9731] | 0.034376 | Fixed | 0.4948 | 0 | |
| Caucasian | 1 | 1.0319 | [0.8068; 1.3198] | 0.802505 | Fixed | NA | NA | |
| pairwise 2 (AA vs. Aa) | Overall | 4 | 0.8681 | [0.6008; 1.2544] | 0.451454 | Random | 0.0171 | 0.7051 |
| Asian | 3 | 0.7741 | [0.4391; 1.3646] | 0.376068 | Random | 0.0244 | 0.7308 | |
| Caucasian | 1 | 1.0471 | [0.8208; 1.3359] | 0.711047 | Fixed | NA | NA | |
| pairwise 3 (Aa vs. aa) | Overall | 4 | 1.0029 | [0.8692; 1.1572] | 0.968297 | Fixed | 0.3458 | 0.0946 |
| Asian | 3 | 1.0639 | [0.7889; 1.4347] | 0.684766 | Fixed | 0.2102 | 0.3588 | |
| Caucasian | 1 | 0.9855 | [0.8373; 1.1599] | 0.860306 | Fixed | NA | NA |
Emphasizing the importance of determining an appropriate sample size is crucial for accurately evaluating statistical significance and ensuring the reliability of findings in genetic association studies, especially when investigating specific polymorphisms_ The estimation of sample size plays a pivotal role in determining the statistical power of these studies
| Gene | SNP | No. of Studies | Cases | Control | α err prob | Power (1-β err prob) |
|---|---|---|---|---|---|---|
| IL1A | rs17561 | 3 | 665 | 954 | 0.02303 | 0.9 |
| IFN-γ | rs2430561 | 5 | 480 | 549 | 0.05343 | 0.9 |
| STOX1 | rs1341667 | 4 | 1738 | 2770 | 0.000495 | 0.9 |
| PPAR-γ | rs1801282 | 3 | 268 | 1828 | 0.06146 | 0.9 |