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Topical Helichrysum italicum Essential Oil-Based Ointment Hastens Excisional Wound Healing by Alleviating Inflammation and Oxidative Stress in Diabetic Rats Cover

Topical Helichrysum italicum Essential Oil-Based Ointment Hastens Excisional Wound Healing by Alleviating Inflammation and Oxidative Stress in Diabetic Rats

Open Access
|Dec 2024

Figures & Tables

Figure 1.

Photograph of H. italicum essential oil – based ointment
Photograph of H. italicum essential oil – based ointment

Figure 2.

Effects of applied ointments on the wound contraction.Values are expressed as mean ± standard deviation (n = 8). a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the wound contraction.Values are expressed as mean ± standard deviation (n = 8). a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Figure 3.

Effects of applied ointments on the markers inflammation: A) TNF-α; B) IL-6; C) IL-10. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the markers inflammation: A) TNF-α; B) IL-6; C) IL-10. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Figure 4.

Effects of applied ointments on the oxidative stress markers in wound tissue: A) TBARS; B) SOD; C) CAT; D) GSH. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;
Effects of applied ointments on the oxidative stress markers in wound tissue: A) TBARS; B) SOD; C) CAT; D) GSH. Values are expressed as mean ± standard deviation (n = 8).a p < 0.05 compared to CTRL group; b p < 0.05 compared to SSD group; c p < 0.05 compared to OINT group; d p < 0.05 compared to HIOINT group. CTRL – control; SSD – silver sulfadiazine; OINT – ointment base; HIOINT – H. italicum ointment;

Organoleptic characteristics and spreadability of ointment with H_ italicum essential oil after 3 and 180 days of preparation

Parameters3 days180 days
ColourYellowYellow
OdourCharacteristic odor of the essential oilCharacteristic odor of the essential oil
ConsistencySemi-solidSemi-solid
HomogenityHomogeneous ointmentHomogeneous ointment
SpreadabilityEasilyEasily

Ointment base and H_ italicum essential oil –based ointment formulations_

IngredientOintment Base (%)Ointment HIEO (%)
HIEO/0.50
Cholesterol5.005.00
Lanolin15.0015.00
Parafinum liquidum15.0015.00
Vaselinum album65.00ad 65.00
DOI: https://doi.org/10.2478/eabr-2024-0012 | Journal eISSN: 2956-2090 | Journal ISSN: 2956-0454
Language: English
Submitted on: Sep 9, 2024
Accepted on: Sep 23, 2024
Published on: Dec 12, 2024
Published by: University of Kragujevac, Faculty of Medical Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2024 Marijana Andjic, Aleksandar Kocovic, Ksenija Vucicevic, Vladimir Jakovljevic, Igor Simanic, Milica Vukasinovic Vesic, Jovana Bradic, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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