Selected Phenolic Compounds in Mainstream Cigarette Smoke, CORESTA Collaborative Study and Recommended Method *

Tayyarah, Rana; Knepper, Douglas; Hauleithner, Alexander

doi:10.2478/cttr-2023-0003

Figures & Tables

Participating laboratories and product volunteers_

Testing participants
Altria Client Services	ITC Ltd.
ARISTA Laboratories	ITG, France
BAT Souza Cruz SA – Brazil	ITL – Reemtsma Cigarettenfabriken
British American Tobacco	Japan Tobacco Inc.
China National Tobacco Corp. Beijing Cigarette Factory	Japan Tobacco International
CNTC – Zhengzhou Tobacco Research Institute	KT&G Corp.
CNTC QC	Labstat International Inc.
Enthalpy Analytical, Inc.	Philip Morris International – Brazil
Essentra Scientific Services	Philip Morris International – Indonesia
Global Laboratory Services, Inc.	R.J. Reynolds Tobacco Co.

Intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data_

		CM7	1R5F	3R4F	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Puff count	Mean ^a	11.78	6.40	10.39	7.00	9.74	7.62	5.81	5.02	9.31	6.54
Puff count	STD ^b	0.56	0.36	0.51	0.40	0.51	0.52	2.63	1.25	2.33	0.37
TPM	Mean	43.36	31.83	44.12	44.11	40.16	45.09	24.62	19.43	33.86	25.79
TPM	STD	6.09	4.07	3.97	4.80	4.62	4.87	12.32	5.91	9.33	3.76
Hydroquinone	Mean	191.17	53.41	89.62	91.44	101.85	114.04	76.44 ^*	43.56	106.50	60.19
	STD	15.16	5.08	7.49	7.99	8.60	6.30	5.32	4.30	9.49	5.47
	r ^c	27.40	11.85	11.67	18.19	15.75	18.27	9.98	9.36	15.97	17.18
	R ^d	49.02	17.74	23.43	27.67	27.88	24.05	17.37	14.67	30.16	21.70
	N ^e	18	17	18	14	18	18	14	18	16	18
Resorcinol	Mean	3.77	1.07 ^*	1.87 ^*	1.35 ^*	2.55	2.77	2.21	2.07	2.70	1.46
	STD	0.79	0.15	0.40	0.41	0.68	0.79	0.48	0.44	0.39	0.42
	r	0.70	0.38	0.45	0.60	0.65	0.75	0.80	0.68	0.71	0.57
	R	2.31	0.55	1.18	1.27	1.99	2.32	1.52	1.39	1.26	1.27
	N	18	14	15	11	17	18	15	17	16	17
Catechol	Mean	198.81	41.73	90.24	76.29	100.18	96.21	79.79 ^*	54.38	127.00 ^*	51.10
	STD	15.11	3.55	6.71	6.52	7.92	9.10	6.22	4.56	6.48	5.27
	r	27.35	9.81	14.58	14.03	14.46	20.19	11.53	12.81	24.78	13.13
	R	48.87	13.26	22.87	22.16	25.68	31.23	20.24	17.16	28.64	18.86
	N	18	17	18	14	18	18	14	18	15	18
Phenol	Mean	43.05	8.20	13.13	36.65	16.99	19.19	12.00	6.64	19.33 ^*	6.93
	STD	3.59	0.91	1.05	1.20	1.46	1.59	1.33	0.63	1.35	0.57
	r	8.93	1.88	3.30	8.39	4.14	4.82	2.96	1.74	4.59	3.13
	R	12.83	3.05	4.17	8.22	5.51	6.21	4.56	2.34	5.58	3.23
	N	18	17	18	14	18	18	15	18	15	18
o-Cresol	Mean	8.67	2.53	4.15	10.88	4.66	5.22	3.14 ^*	1.90	4.53 ^*	1.97
	STD	1.14	0.28	0.50	0.99	0.60	0.60	0.38	0.22	0.41	0.28
	r	1.89	0.58	0.96	2.52	1.05	1.22	0.66	0.52	1.15	0.91
	R	3.62	0.93	1.64	3.58	1.93	2.01	1.22	0.78	1.55	1.13
	N	18	16	18	14	18	18	14	17	15	17
m-Cresol	Mean	7.08	1.78	3.25	7.14	3.58	3.90	2.65	1.58	3.91	1.67
	STD	1.07	0.22	0.45	0.74	0.51	0.37	0.43	0.22	0.37	0.35
	r	1.27	0.48	0.72	1.49	0.68	0.90	0.64	0.45	0.84	0.69
	R	3.22	0.76	1.42	2.47	1.55	1.31	1.34	0.74	1.28	1.16
	N	17	16	17	13	17	16	14	17	16	17
p-Cresol	Mean	20.16 ^*	5.53	8.54 ^*	21.25	9.99 ^*	10.77 ^*	6.49	4.14	9.08 ^*	4.12 ^*
	STD	2.21	0.72	0.72	2.32	1.10	1.06	0.81	0.36	0.82	0.40
	r	3.58	1.09	1.78	4.29	1.98	2.19	1.31	0.91	1.84	1.82
	R	6.97	2.24	2.56	7.55	3.56	3.55	2.56	1.30	2.82	1.98
	N	16	16	16	13	16	16	14	17	15	16

Elution program_a

Time (min)	Composition
Time (min)	% A	% B
0	78	22
8	78	22
8.5	55	45
21	55	45
22	0	100
28	0	100
(Equilibrate 6 min)

Non-intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data_

		CM7	1R5F	3R4F	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Puff count	Mean ^a	8.45	6.90	8.28	5.66	7.88	6.91	5.19	4.54	6.82	6.16
Puff count	STD ^b	0.35	0.27	0.33	0.27	0.36	0.34	2.35	1.10	1.65	0.25
TPM	Mean	16.12	1.96	9.85	13.46	8.85	10.24	3.26	1.69	10.64	1.17
TPM	STD	1.07	0.32	0.78	1.78	0.75	0.84	1.71	0.52	2.90	0.31
Hydroquinone	Mean	91.82	8.45	32.84	42.78	36.98	46.88	20.38 ^*	9.30	46.69	8.09
	STD	7.47	1.33	2.78	3.85	3.43	3.60	3.26	1.19	4.08	0.96
	r ^c	12.14	1.58	4.27	8.92	5.97	6.72	2.89	1.78	6.31	1.41
	R ^d	23.58	3.97	8.67	13.40	10.99	11.75	9.49	3.68	12.73	2.97
	N ^e	18	17	18	14	18	18	14	18	16	18
Resorcinol	Mean	1.79 ^*	0.13 ^*	0.63 ^*	0.66 ^*	0.80 ^*	0.97 ^*	0.51	0.35	1.19 ^*	0.12 ^*
	STD	0.30	0.04	0.15	0.21	0.16	0.18	0.09	0.05	0.21	0.03 ^*
	r	0.52	0.09	0.19	0.37	0.29	0.29	0.18	0.14	0.22	0.10
	R	0.95	0.14	0.46	0.67	0.51	0.58	0.30	0.17	0.63	0.13
	N	17	13	15	12	16	16	13	15	15	12
Catechol	Mean	96.39	8.13	36.77	35.30	39.87	42.61	24.17	12.89	58.19	7.84
	STD	4.13	0.62	1.72	1.53	2.45	2.16	2.41	0.68	3.09	0.59
	r	13.39	1.60	5.02	7.32	6.84	4.91	4.77	1.98	7.58	1.88
	R	16.65	2.25	6.60	7.83	9.19	7.46	7.99	2.61	11.00	2.35
	N	18	17	18	14	18	17	15	18	16	18
Phenol	Mean	27.60 ^*	0.93	7.06	19.97	9.64	12.47	4.58	1.21 ^*	13.91	0.49 ^*
	STD	2.94	0.19	0.80	1.49	1.28	1.15	1.11	0.25	1.42	0.11
	r	4.17	0.44	1.59	4.12	2.43	2.52	1.24	0.36	3.04	0.29
	R	9.05	0.65	2.64	5.57	4.20	3.93	3.30	0.77	4.81	0.39
	N	16	14	18	14	18	18	15	16	16	13
o-Cresol	Mean	5.63	0.38	2.33	5.75	2.62	3.30	1.34	0.42 ^*	3.40	0.21
	STD	0.68	0.08	0.27	0.65	0.37	0.35	0.26	0.10	0.43	0.07
	r	0.96	0.15	0.54	1.22	0.63	0.67	0.29	0.15	0.66	0.12
	R	2.08	0.25	0.90	2.11	1.18	1.14	0.78	0.31	1.35	0.23
	N	18	15	18	14	18	18	15	16	16	13
m-Cresol	Mean	4.57	0.32	1.83	3.73	2.03	2.50	1.10	0.38	2.78	0.24
	STD	0.67	0.10	0.18	0.45	0.33	0.29	0.22	0.07	0.28	0.12
	r	0.69	0.12	0.37	0.77	0.42	0.43	0.23	0.11	0.53	0.10
	R	1.98	0.29	0.61	1.43	0.99	0.90	0.64	0.23	0.92	0.36
	N	17	13	17	13	17	17	14	17	16	13
p-Cresol	Mean	12.45	0.73	4.56	10.40	5.44	6.49	2.49	0.88	6.25	0.44
	STD	1.24	0.14	0.51	0.87	0.71	0.60	0.50	0.13	0.73	0.13
	r	1.63	0.27	0.75	2.24	1.19	1.07	0.47	0.20	1.02	0.16
	R	3.76	0.46	1.58	3.16	2.26	1.93	1.47	0.40	2.24	0.39
	N	17	16	17	13	17	17	14	17	16	17

Study samples_

Sample ID	Blend description	Approximate TPM ISO 3308 regime (mg/cig)^a	Approximate TPM ISO 20778 regime (mg/cig)
CM7	Virginia	16.1	43.4
KR 1R5F	American	2.0	31.8
KR 3R4F	American	9.9	44.1
Sample 1	Dark air-cured	13.5	44.1
Sample 2	American	8.9	40.2
Sample 3	American	10.2	45.1
Sample 4	Virginia	4.0	30.3
Sample 5	Virginia	1.8	20.6
Sample 6	Virginia	11.3	36.1
Sample 7	Charcoal filtered	1.2	25.8

Fluorescence detector settings_

Time initial	Excitation (nm)	Emission (nm)
0.0	280	310
12.4	280	310
12.5	274	298
23.0	274	298
24.0	280	310
28.0	280	310

Selected Phenolic Compounds in Mainstream Cigarette Smoke, CORESTA Collaborative Study and Recommended Method *

Figures & Tables

Participating laboratories and product volunteers_

Intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data_

Elution program_a

Non-intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data_

Study samples_

Fluorescence detector settings_

Paradigm

My account