Selected Phenolic Compounds in Mainstream Cigarette Smoke, CORESTA Collaborative Study and Recommended Method *

Testing participants
Altria Client Services	ITC Ltd.
ARISTA Laboratories	ITG, France
BAT Souza Cruz SA – Brazil	ITL – Reemtsma Cigarettenfabriken
British American Tobacco	Japan Tobacco Inc.
China National Tobacco Corp. Beijing Cigarette Factory	Japan Tobacco International
CNTC – Zhengzhou Tobacco Research Institute	KT&G Corp.
CNTC QC	Labstat International Inc.
Enthalpy Analytical, Inc.	Philip Morris International – Brazil
Essentra Scientific Services	Philip Morris International – Indonesia
Global Laboratory Services, Inc.	R.J. Reynolds Tobacco Co.

Product Volunteers
British American Tobacco Germany GmbH	Japan Tobacco – Japan
China National Tobacco Corp. Beijing Cigarette Factory	Philip Morris International – Neuchatel, Switzerland
ITG Altadis	Shanghai Tobacco Group Co. Ltd

Table 2.

Study samples.

Sample ID	Blend description	Approximate TPM ISO 3308 regime (mg/cig)^a	Approximate TPM ISO 20778 regime (mg/cig)
CM7	Virginia	16.1	43.4
KR 1R5F	American	2.0	31.8
KR 3R4F	American	9.9	44.1
Sample 1	Dark air-cured	13.5	44.1
Sample 2	American	8.9	40.2
Sample 3	American	10.2	45.1
Sample 4	Virginia	4.0	30.3
Sample 5	Virginia	1.8	20.6
Sample 6	Virginia	11.3	36.1
Sample 7	Charcoal filtered	1.2	25.8

1a Based on the average TPM values from the study reported in this report

Table 3.

Fluorescence detector settings.

Time initial	Excitation (nm)	Emission (nm)
0.0	280	310
12.4	280	310
12.5	274	298
23.0	274	298
24.0	280	310
28.0	280	310

Table 4.

Elution program.^a

Time (min)	Composition
Time (min)	% A	% B
0	78	22
8	78	22
8.5	55	45
21	55	45
22	0	100
28	0	100
(Equilibrate 6 min)

1a Linear gradient

Table 5.

Non-intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data.

		CM7	1R5F	3R4F	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Puff count	Mean ^a	8.45	6.90	8.28	5.66	7.88	6.91	5.19	4.54	6.82	6.16
Puff count	STD ^b	0.35	0.27	0.33	0.27	0.36	0.34	2.35	1.10	1.65	0.25
TPM	Mean	16.12	1.96	9.85	13.46	8.85	10.24	3.26	1.69	10.64	1.17
TPM	STD	1.07	0.32	0.78	1.78	0.75	0.84	1.71	0.52	2.90	0.31
Hydroquinone	Mean	91.82	8.45	32.84	42.78	36.98	46.88	20.38 ^*	9.30	46.69	8.09
	STD	7.47	1.33	2.78	3.85	3.43	3.60	3.26	1.19	4.08	0.96
	r ^c	12.14	1.58	4.27	8.92	5.97	6.72	2.89	1.78	6.31	1.41
	R ^d	23.58	3.97	8.67	13.40	10.99	11.75	9.49	3.68	12.73	2.97
	N ^e	18	17	18	14	18	18	14	18	16	18
Resorcinol	Mean	1.79 ^*	0.13 ^*	0.63 ^*	0.66 ^*	0.80 ^*	0.97 ^*	0.51	0.35	1.19 ^*	0.12 ^*
	STD	0.30	0.04	0.15	0.21	0.16	0.18	0.09	0.05	0.21	0.03 ^*
	r	0.52	0.09	0.19	0.37	0.29	0.29	0.18	0.14	0.22	0.10
	R	0.95	0.14	0.46	0.67	0.51	0.58	0.30	0.17	0.63	0.13
	N	17	13	15	12	16	16	13	15	15	12
Catechol	Mean	96.39	8.13	36.77	35.30	39.87	42.61	24.17	12.89	58.19	7.84
	STD	4.13	0.62	1.72	1.53	2.45	2.16	2.41	0.68	3.09	0.59
	r	13.39	1.60	5.02	7.32	6.84	4.91	4.77	1.98	7.58	1.88
	R	16.65	2.25	6.60	7.83	9.19	7.46	7.99	2.61	11.00	2.35
	N	18	17	18	14	18	17	15	18	16	18
Phenol	Mean	27.60 ^*	0.93	7.06	19.97	9.64	12.47	4.58	1.21 ^*	13.91	0.49 ^*
	STD	2.94	0.19	0.80	1.49	1.28	1.15	1.11	0.25	1.42	0.11
	r	4.17	0.44	1.59	4.12	2.43	2.52	1.24	0.36	3.04	0.29
	R	9.05	0.65	2.64	5.57	4.20	3.93	3.30	0.77	4.81	0.39
	N	16	14	18	14	18	18	15	16	16	13
o-Cresol	Mean	5.63	0.38	2.33	5.75	2.62	3.30	1.34	0.42 ^*	3.40	0.21
	STD	0.68	0.08	0.27	0.65	0.37	0.35	0.26	0.10	0.43	0.07
	r	0.96	0.15	0.54	1.22	0.63	0.67	0.29	0.15	0.66	0.12
	R	2.08	0.25	0.90	2.11	1.18	1.14	0.78	0.31	1.35	0.23
	N	18	15	18	14	18	18	15	16	16	13
m-Cresol	Mean	4.57	0.32	1.83	3.73	2.03	2.50	1.10	0.38	2.78	0.24
	STD	0.67	0.10	0.18	0.45	0.33	0.29	0.22	0.07	0.28	0.12
	r	0.69	0.12	0.37	0.77	0.42	0.43	0.23	0.11	0.53	0.10
	R	1.98	0.29	0.61	1.43	0.99	0.90	0.64	0.23	0.92	0.36
	N	17	13	17	13	17	17	14	17	16	13
p-Cresol	Mean	12.45	0.73	4.56	10.40	5.44	6.49	2.49	0.88	6.25	0.44
	STD	1.24	0.14	0.51	0.87	0.71	0.60	0.50	0.13	0.73	0.13
	r	1.63	0.27	0.75	2.24	1.19	1.07	0.47	0.20	1.02	0.16
	R	3.76	0.46	1.58	3.16	2.26	1.93	1.47	0.40	2.24	0.39
	N	17	16	17	13	17	17	14	17	16	17

1a Mean: mean yield (μg/cig) except TPM (mg/cig) and puff count (pc)

1b STD: overall standard deviation

1c r: repeatability (μg/cig at 95% confidence interval)

1d R: reproducibility (μg/cig at 95% confidence interval)

1e N: number of datasets used for r and R determination after removal of outliers and non-quantifiable values

1* Values calculated after removal of outlier (s)

Table 6.

Intense smoking regime – Inter-laboratory mean yields, repeatability (r) and reproducibility (R) data.

		CM7	1R5F	3R4F	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Puff count	Mean ^a	11.78	6.40	10.39	7.00	9.74	7.62	5.81	5.02	9.31	6.54
Puff count	STD ^b	0.56	0.36	0.51	0.40	0.51	0.52	2.63	1.25	2.33	0.37
TPM	Mean	43.36	31.83	44.12	44.11	40.16	45.09	24.62	19.43	33.86	25.79
TPM	STD	6.09	4.07	3.97	4.80	4.62	4.87	12.32	5.91	9.33	3.76
Hydroquinone	Mean	191.17	53.41	89.62	91.44	101.85	114.04	76.44 ^*	43.56	106.50	60.19
	STD	15.16	5.08	7.49	7.99	8.60	6.30	5.32	4.30	9.49	5.47
	r ^c	27.40	11.85	11.67	18.19	15.75	18.27	9.98	9.36	15.97	17.18
	R ^d	49.02	17.74	23.43	27.67	27.88	24.05	17.37	14.67	30.16	21.70
	N ^e	18	17	18	14	18	18	14	18	16	18
Resorcinol	Mean	3.77	1.07 ^*	1.87 ^*	1.35 ^*	2.55	2.77	2.21	2.07	2.70	1.46
	STD	0.79	0.15	0.40	0.41	0.68	0.79	0.48	0.44	0.39	0.42
	r	0.70	0.38	0.45	0.60	0.65	0.75	0.80	0.68	0.71	0.57
	R	2.31	0.55	1.18	1.27	1.99	2.32	1.52	1.39	1.26	1.27
	N	18	14	15	11	17	18	15	17	16	17
Catechol	Mean	198.81	41.73	90.24	76.29	100.18	96.21	79.79 ^*	54.38	127.00 ^*	51.10
	STD	15.11	3.55	6.71	6.52	7.92	9.10	6.22	4.56	6.48	5.27
	r	27.35	9.81	14.58	14.03	14.46	20.19	11.53	12.81	24.78	13.13
	R	48.87	13.26	22.87	22.16	25.68	31.23	20.24	17.16	28.64	18.86
	N	18	17	18	14	18	18	14	18	15	18
Phenol	Mean	43.05	8.20	13.13	36.65	16.99	19.19	12.00	6.64	19.33 ^*	6.93
	STD	3.59	0.91	1.05	1.20	1.46	1.59	1.33	0.63	1.35	0.57
	r	8.93	1.88	3.30	8.39	4.14	4.82	2.96	1.74	4.59	3.13
	R	12.83	3.05	4.17	8.22	5.51	6.21	4.56	2.34	5.58	3.23
	N	18	17	18	14	18	18	15	18	15	18
o-Cresol	Mean	8.67	2.53	4.15	10.88	4.66	5.22	3.14 ^*	1.90	4.53 ^*	1.97
	STD	1.14	0.28	0.50	0.99	0.60	0.60	0.38	0.22	0.41	0.28
	r	1.89	0.58	0.96	2.52	1.05	1.22	0.66	0.52	1.15	0.91
	R	3.62	0.93	1.64	3.58	1.93	2.01	1.22	0.78	1.55	1.13
	N	18	16	18	14	18	18	14	17	15	17
m-Cresol	Mean	7.08	1.78	3.25	7.14	3.58	3.90	2.65	1.58	3.91	1.67
	STD	1.07	0.22	0.45	0.74	0.51	0.37	0.43	0.22	0.37	0.35
	r	1.27	0.48	0.72	1.49	0.68	0.90	0.64	0.45	0.84	0.69
	R	3.22	0.76	1.42	2.47	1.55	1.31	1.34	0.74	1.28	1.16
	N	17	16	17	13	17	16	14	17	16	17
p-Cresol	Mean	20.16 ^*	5.53	8.54 ^*	21.25	9.99 ^*	10.77 ^*	6.49	4.14	9.08 ^*	4.12 ^*
	STD	2.21	0.72	0.72	2.32	1.10	1.06	0.81	0.36	0.82	0.40
	r	3.58	1.09	1.78	4.29	1.98	2.19	1.31	0.91	1.84	1.82
	R	6.97	2.24	2.56	7.55	3.56	3.55	2.56	1.30	2.82	1.98
	N	16	16	16	13	16	16	14	17	15	16

1 ^a Mean: mean yield (μg/cig) except TPM (mg/cig) and puff count (pc)

1 ^b STD: overall standard deviation

1 ^c r: repeatability (μg/cig at 95% confidence interval)

1 ^d R: reproducibility (μg/cig at 95% confidence interval)

1 ^e N: number of datasets used for r and R determination after removal of outliers and non-quantifiable values

1 ^* Values calculated after removal of outlier (s)

References

Authors

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Articles in this issue

DOI: https://doi.org/10.2478/cttr-2023-0003 | Journal eISSN: 2719-9509 (formerly 1612-9237)

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Language: English, French, German

Page range: 18 - 25

Submitted on: Jun 24, 2022

Accepted on: Jan 31, 2023

Published on: Apr 4, 2023

Published by: Beiträge zur Tabakforschung GmbH

In partnership with: Paradigm Publishing Services

Keywords:

Cigarette,

harmful and potentially harmful constituents,

HPHC,

smoke,

phenol,

Related subjects:

© 2023 Rana Tayyarah, Douglas Knepper, Alexander Hauleithner, published by Beiträge zur Tabakforschung GmbH
This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License.

Volume 32 (2023): Issue 1 (March 2023)