HPHC Testing of Tobacco and Smoke to Examine Cigarette Temporal Variability

Tayyarah, Rana; Morton, Michael J.; Flora, Jason W.

doi:10.2478/cttr-2022-0012

Figures & Tables

Tobacco NNN for Product 9 across all batches compared to repeat testing results for 3R4F across the same timeframes. Shaded regions represent repeat testing of the same batch. A, B1, C are samples collected within 1 week of production. Samples B2, D, E, and F are samples collected quarterly for 1 year. Samples B3, G, and H are samples collected annually for 3 years. 3R4F was one batch collected, shipped, and tested like the production samples. Percent values displayed are the spread in timepoint replicate averaged values to show relative variability of batches within a study phase.

Non-intense smoke BaP for product 1 across all batches compared to repeat testing results for 3R4F across the same timeframes. Shaded regions represent repeat testing of the same batch. A, B1, C are samples collected within 1 week of production. Samples B2, D, E, and F are samples collected quarterly for 1 year. Samples B3, G, and H are samples collected annually for 3 years. 3R4F was one batch collected, shipped, and tested like the production samples. Percent values displayed are the spread in timepoint replicate averaged values to show relative variability of batches within a study phase.

Product 2 CO yields from non-intense smoking for long-term variability testing as an illustration of interactive effects among laboratories and of the magnitude of differences that may be expected from testing multiple batches of the same product in different laboratories using standardized methodology_

Product 2 Non-intense CO (mg/cig)Phase 3 samples				Product range	Laboratory range

	Batch			%	%
Lab	B3	G	H	–	–
1	4.41	4.11	4.64	–	–
4	3.85	3.70	3.87	–	–
5	3.56	3.64	3.48	–	–
6	4.70	4.59	4.87	–	–
7	3.92	3.75	4.44	–	–
8	4.00	3.75	4.43	–	–
9	3.62	3.16	3.92	–	–
10	3.53	3.70	3.50	–	–
11	4.14	4.02	4.66	–	–
Average	3.97	3.82	4.20	9.4	29.0

Relative range (%) among time point for each product sample for select parameters and constituents_

		Sample

	Timepoint	1	2	3	4	6	7	8	9	3R4F
Physical parameters

Cigarette weight as-is (mg/cig)	Short	0.8%	0.6%	0.2%	0.9%	0.2%	0.3%	0.3%	0.6%	NC^a
	Medium	1.3%^*	1.7%^*	1.2%^*	1.4%^*	NC	1.6%^*	0.9%^*	2.6%^*	0.2%
	Long	3.4%^*	0.3%	0.4%	1.9%^*	0.7%	3.2%^*	3.8%	1.1%	0.4%
Filter tip ventilation (%)	Short	3.4%^*	6.0%^*	0.1%	8.4%^*	0.9%	19.7%^*	1.3%	0.9%	NC
	Medium	1.8%^*	8%^*	0.4%^*	8.2%^*	NC	27.1%^*	5.5%^*	5.0%^*	2.4%
	Long	10.4%^*	8.6%	0.1%	9.0%^*	2.8%	14.0%^*	0.7%	1.2%	6.6%

Tobacco constituents

NNN (ng/g)	Short	1.4%	7.4%	11.9%	3.1%	3.0%	6.5%	14.7%^*	27.1%^*	3.8%
	Medium	41.9%^*	31.4%^*	51%^*	14.6%^*	29.4%^*	34.5%^*	30.5%^*	81.5%^*	2.8%
	Long	34.0%^*	31.5%^*	NCS ^b	2.3%	13.8%^*	28.1%^*	NCS	57.2%^*	3.2%
NNK (ng/g)	Short	8.1%	24.7%^*	8.1%	12.6%^*	10.3%	2.0%	15.4%^*	32.9%^*	3.3%
	Medium	62.1%^*	53.5%^*	47.4%^*	7.5%	37.8%^*	33.9%^*	28.2%^*	58.9%^*	9.2%
	Long	34.7%^*	20.9%	NCS	6.4%	36.0%	21.4%	NCS	54.4%	8.9%^*
Nicotine (μg/g)	Short	2.2%^*	1.0%	4.8%^*	3.1%^*	1.0%	4.8%^*	2.1%^*	3.9%^*	0.4%
	Medium	9.8%^*	13.8%^*	4.1%^*	3.7%^*	5.2%^*	5.2%^*	3.9%^*	8.6%^*	1.7%^*
	Long	8.3%^*	5.6%^*	14.0%^*	2.5%^*	2.8%^*	4.4%^*	0.3%	7.6%^*	0.1%
Ammonia (μg/g)	Short	6.7%^*	7.5%^*	9.4%^*	6.7%^*	1.5%	0.9%	34.4%^*	12.8%^*	3.1%
	Medium	11.6%^*	15.0%^*	63.7%^*	8.6%^*	21.1%^*	11.0%^*	66.5%^*	10.3%^*	4.8%^*
	Long	12.5%^*	2.1%	NCS	24.6%^*	10.6%^*	10.4%^*	NCS	17.2%^*	1.0%

Smoke constituent – Non-intense regime

Ammonia (μg/cig)	Short	0.9%	< LOQ	2.3%	8.3%	2.6%	< LOQ	8.7%	2.1%	5.3%
	Medium	18.8%^*	< LOQ	5.5%	5.0%	28.3%^*	< LOQ	17.7%	19.2%	6.0%
	Long	14.7%	8.8%	NCS	7.4%	21.5%	< LOQ	NCS	7.5%	9.2%
Acetaldehyde (μg/cig)	Short	5.0%	9.5%	2.4%	6.5%	6.9%^*	51.1%^*	5.1%	7.4%	6.8%
	Medium	11.3%	11.4%	6.5%	9.3%^*	3.2%	67.3%^*	3.9%	13.2%^*	11.6%^*
	Long	12.4%^*	7.0%	0.5%	5.7%	3.6%	21.6%	0.7%	2.5%	3.3%
Total HCN (μg/cig)	Short	5.8%	8.5%	2.9%	15.9%^*	17.9%^*	< LOQ	15.7%^*	16.5%	4.5%
	Medium	15.9%^*	22.9%	8.0%	8.1%	9.4%^*	< LOQ	30.1%^*	16.4%^*	7.7%
	Long	20.5%^*	31.0%	NCS	13.3%	15.3%	< LOQ	NCS	6.0%	12.1%
4-Aminobiphenyl (ng/cig)	Short	3.2%	5.5%^*	3.6%	2.8%	2.0%	17.6%^*	4.9%	1.1%	4.5%
	Medium	4.5%	2.4%	4.5%	6.5%	6.4%^*	51.3%^*	20.1%^*	5.3%	5.4%
	Long	7.4%	6.4%	NCS	11.4%^*	2.6%	16.9%^*	NCS	5.2%	3.9%
BaP (ng/cig)	Short	2.2%	3.0%	1.7%	0.3%	1.9%	4.2%	2.2%	2.6%	1.1%
	Medium	4.1%	3.6%	2.3%	4.0%	6.1%^*	10.4%	2.2%	4.3%	1.6%
	Long	8.1%	6.9%	3.9%	NC	6.3%	29.9%^*	7.5%	3.5%	1.7%
NNN (ng/cig)	Short	13.4%	14.3%^*	9.7%	4.0%	11.3%	27.1%^*	15.6%	13.1%	2.9%
	Medium	36.9%^*	11.9%	21.9%	5.7%	8.4%	54.9%^*	51.3%^*	44.7%^*	16.5%
	Long	33.1%^*	51.7%^*	NCS	0.7%	12.4%	25%^*	NCS	45.9%^*	10.8%
NNK (ng/cig)	Short	18.0%	14.1%^*	8.2%	8.0%	9.9%	40.2%^*	12.0%	26.8%	4.0%
	Medium	44.6%^*	31.7%	25.1%	11.6%^*	17.6%	62.9%^*	34.4%	36%^*	12.6%
	Long	28.1%^*	47.7%^*	NCS	3.4%	12.2%	34.0%	NCS	32.9%^*	12.7%^*
Benzene (μg/cig)	Short	6.4%	16.2%^*	2.8%	3.9%	2.9%	23%^*	3.7%	4.4%	1.3%
	Medium	13%^*	19%^*	6.2%	6.2%	7.7%	81.8%^*	11.5%^*	9.0%	10.6%^*
	Long	14.5%^*	8.7%^*	NCS	0.3%	1.7%	28.1%^*	NCS	6.5%	4.1%

Smoke constituent - Intense regime

Ammonia (μg/cig)	Short	10.6%	9.9%	0.8%	1.1%	7.2%	3.7%	13.4%^*	5.9%	7.2%
	Medium	19.0%	47.6%	22.8%	12.8%	30.5%^*	7.3%	45.2%^*	15.2%	14.1%
	Long	1.6%	9%^*	NCS	1.9%	22.9%^*	11.0%	NCS	3.0%	7.7%
Acetaldehyde (μg/cig)	Short	1.7%	2.6%	3.5%	3.3%	1.8%	2.7%	4.9%	3.0%	1.4%
	Medium	2.7%	5.5%	6.0%	4.9%	8.9%^*	6.7%	6.9%	4.7%	3.5%
	Long	2.4%	3.8%	1.0%	2.9%	9.4%^*	3.9%	10.8%^*	0.5%	0.4%
Total HCN (μg/cig)	Short	5.8%	5.2%	7.4%	1.4%	9.6%	4.2%	1.2%	13.6%	4.7%
	Medium	6.1%	15.6%^*	9.2%	2.9%	9.5%	8.9%	19.2%^*	15.8%^*	7.5%
	Long	13.4%^*	10.5%^*	NCS	10.8%	9.4%	4.7%	NCS	13.0%	2.8%
4-Aminobiphenyl (ng/cig)	Short	3.9%	3.2%	2.0%	4.8%	0.7%	0.4%	1.7%	4.7%	2.4%
	Medium	10.9%	14.3%^*	4.7%	4.1%	8.8%^*	5.2%	17.3%^*	5.1%	4.6%
	Long	10.5%	9.2%	NCS	6.8%^*	13.7%	9.6%^*	NCS	4.9%	7.2%
BaP (ng/cig)	Short	2.2%	2.9%	2.8%	2.1%	1.8%	0.4%	3.8%	3.1%	2.3%
	Medium	3.7%	3.6%	7.2%^*	2.1%	5.6%	2.5%	4.4%	7.3%	6.1%^*
	Long	5.4%	5.4%	2.5%	NC	7.9%	2.5%	8.5%	5.5%	1.4%
NNN (ng/cig)	Short	1.2%	17.3%^*	19.2%^*	18.9%^*	5.3%	3.5%	10.5%	10.1%	4.3%
	Medium	37.2%^*	26.6%^*	62.6%^*	4.6%	20.9%	23.7%^*	13.9%	52.9%^*	10.3%
	Long	22.9%	52.9%^*	NCS	5.5%	10.6%	30.5%^*	NCS	40.6%^*	12.5%
NNK (ng/cig)	Short	6.6%	2.3%	14.8%	9.2%	11.1%	15.8%	21.3%^*	21.6%^*	5.7%
	Medium	34.5%^*	41.8%^*	18.7%^*	8.5%	16.3%	25.8%^*	33.6%	26.2%^*	12.5%
	Long	26.9%	49.5%^*	NCS	6.6%	25.4%^*	11.8%	NCS	26.6%^*	12.0%
Benzene (μg/cig)	Short	5.0%	2.3%	8.5%	3.4%	6.2%	4.7%	10.5%^*	5.0%	2.3%
	Medium	4.2%	5.0%	5.1%	3.9%	4.3%	4.7%	9.5%^*	7.0%	2.4%
	Long	3.7%	5.1%	NCS	2.3%	1.5%	1.3%	NCS	3.2%	4.9%

Study design and batch designations_

Batch designation Study phase	A	B1, B2, B3 ^a	C	D	E	F	G	H
Phase 1	Week 1	Week 1	Week 1
Short-term	shift 1	shift 2	shift 3
Phase 2		Year 1		Year 1	Year 1	Year 1
Medium-term		quarter 1		quarter 2	quarter 3	quarter 4
Phase 3		Year 1					Year 2	Year 3
Long-term

Sample descriptions_

Sample code	Blend	~Non-intense “tar”	Comment
1	American	10 mg	–
2	American	3 mg	Charcoal filter
3	Virginia	10 mg	–
4	American	10 mg	–
5 ^a	NA	NA	Withdrawn
6	American	14 mg	–
7	American	1 mg	–
8	Virginia	8 mg	–
9	American	7 mg
10 (3R4F)	American	8 mg	Single batch study reference
11 (1R6F)	American	8 mg	Informational

HPHC Testing of Tobacco and Smoke to Examine Cigarette Temporal Variability

Figures & Tables

Figure 1

Figure 2

Relative range (%) among time point for each product sample for select parameters and constituents_

Study design and batch designations_

Sample descriptions_

Paradigm

My account