![Extraction of Benzo[a]pyrene from Moist Snuff with Water or Artificial Saliva (Part 2) Cover](https://paradigm-files.s3.eu-central-1.amazonaws.com/CTTR/cover-image.jpg)
Figure 1
Typical chromatogram of a moist snuff sample (corresponding to about 7.6 ng/mL BaP).
Figure 2
Typical chromatogram of a processed water extract of 5 g moist snuff sample (corresponding to about 3.4 ng/mL BaP in solution).
Table 1
Recovery study for water spiked with BaP.
| Added BaP (ng) | Analyzed BaP (ng) | Recovery (%) |
|---|---|---|
| 5.0 | 5.104 | 102.1 |
| 5.0 | 5.048 | 101.0 |
| 5.0 | 4.954 | 99.1 |
| 0.156 | 0.162 | 104.0 |
| 0.156 | 0.147 | 94.0 |
| 0.156 | 0.157 | 100.6 |
Table 2
Moist snuff samples and their moisture content in percent.
| No. | Moist snuff type | Moisture (%) | RSD (%) |
|---|---|---|---|
| 1 | Kodiak Premium wintergreen | 50.05 | 0.42 |
| 2 | Grizzly Premium wintergreen (long cut) | 50.74 | 2.62 |
| 3 | Grizzly Premium wintergreen (fine cut) | 50.35 | 0.97 |
| 4 | Copenhagen Snuff original (fine cut) | 53.19 | 2.11 |
| 5 | Copenhagen mint (long cut) | 54.02 | 1.65 |
| 6 | Skoal classic wintergreen (long cut) | 53.60 | 1.40 |
| 7 | Red Seal natural (fine cut) | 53.56 | 0.04 |
| 8 | Long Horn natural (fine cut) | 50.70 | 0.00 |
| 9 | Red Man moist snuff natural (fine cut) | 47.13 | 1.23 |
Table 3
Levels of BaP (ng/g) on a dry weight basis for moist snuff.
| No. | Moist snuff type | BaP (ng/g) | RSD (%) |
|---|---|---|---|
| 1 | Kodiak premium wintergreen | 152.82 | 0.82 |
| 2 | Grizzly premium wintergreen (long cut) | 121.09 | 0.50 |
| 3 | Grizzly premium wintergreen (fine cut) | 85.98 | 1.61 |
| 4 | Copenhagen snuff original (fine cut) | 83.50 | 1.89 |
| 5 | Copenhagen mint (long cut) | 59.63 | 2.80 |
| 6 | Skoal classic wintergreen (long cut) | 99.11 | 0.59 |
| 7 | Red Seal natural (fine cut) | 73.88 | 0.48 |
| 8 | Long Horn natural (fine cut) | 45.82 | 1.10 |
| 9 | Red Man moist snuff natural (fine cut) | 65.60 | 2.42 |
Table 4
Levels of BaP (ng/g) detected in 100 mL water extract.
| No. | Moist snuff type | BaP (ng/g) | RSD (%) |
|---|---|---|---|
| 1 | Kodiak premium wintergreen | 2.08 | 3.39 |
| 2 | Grizzly premium wintergreen (long cut) | 1.22 | 4.63 |
| 3 | Grizzly premium wintergreen (fine cut) | 1.45 | 7.42 |
| 4 | Copenhagen snuff original (fine cut) | 1.36 | 5.16 |
| 5 | Copenhagen mint (long cut) | 0.81 | 6.78 |
| 6 | Skoal classic wintergreen (long cut) | 1.29 | 2.40 |
| 7 | Red Seal natural (fine cut) | 0.78 | 1.32 |
| 8 | Long Horn natural (fine cut) | 0.78 | 2.67 |
| 9 | Red Man moist snuff natural (fine cut) | 0.83 | 1.10 |
Table 5
Levels of BaP (ng/g) detected in 100 mL artificial saliva extract.
| No. | Moist snuff type | BaP (ng/g) | RSD (%) |
|---|---|---|---|
| 1 | Kodiak premium wintergreen | 3.65 | 0.70 |
| 2 | Grizzly premium wintergreen (long cut) | 2.63 | 1.42 |
| 3 | Grizzly premium wintergreen (fine cut) | 2.49 | 1.94 |
| 4 | Copenhagen snuff original (fine cut) | 2.79 | 2.35 |
| 5 | Copenhagen mint (long cut) | 1.69 | 4.09 |
| 6 | Skoal classic wintergreen (long cut) | 2.00 | 4.66 |
| 7 | Red Seal natural (fine cut) | 2.02 | 3.77 |
| 8 | Long Horn natural(fine cut) | 1.79 | 2.96 |
| 9 | Red Man moist snuff natural (fine cut) | 2.46 | 2.97 |
Table 6
Percent from the initial level of BaP extracted in 100 mL water.
| No. | Moist snuff type | % BaP in water from the initial |
|---|---|---|
| 1 | Kodiak premium wintergreen | 1.36 |
| 2 | Grizzly premium wintergreen (long cut) | 1.01 |
| 3 | Grizzly premium wintergreen (fine cut) | 1.69 |
| 4 | Copenhagen snuff original (fine cut) | 1.62 |
| 5 | Copenhagen mint (long cut) | 1.36 |
| 6 | Skoal classic wintergreen (long cut) | 1.30 |
| 7 | Red Seal natural (fine cut) | 1.05 |
| 8 | Long Horn natural (fine cut) | 1.70 |
| 9 | Red Man moist snuff natural (fine cut) | 1.27 |
Table 7
Percent from the initial level of BaP extracted in 100 mL artificial saliva.
| No. | Moist snuff type | % BaP in artificial saliva from the initial |
|---|---|---|
| 1 | Kodiak premium wintergreen | 2.39 |
| 2 | Grizzly premium wintergreen (long cut) | 2.17 |
| 3 | Grizzly premium wintergreen (fine cut) | 2.89 |
| 4 | Copenhagen snuff original (fine cut) | 3.34 |
| 5 | Copenhagen mint (long cut) | 2.83 |
| 6 | Skoal classic wintergreen (long cut) | 2.02 |
| 7 | Red Seal natural (fine cut) | 2.73 |
| 8 | Long Horn natural (fine cut) | 3.92 |
| 9 | Red Man moist snuff natural (fine cut) | 3.75 |