Development of Caudal Fin Cell Line from Hill Trout Barilius Bendelisis (Hamilton, 1807) for Cytotoxicity and Transfection Studies

Kumar, Murali Sanjeev; Soni, Pankaj; Kumar, Ravindra; Singh, Neha; Srivastava, Shreya; Mishra, Akhilesh Kumar; Singh, Vijay Kumar; Kushwaha, Basdeo

doi:10.2478/cjf-2021-0002

Abstract

A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/pollutant and gene expression.

References

Avella, M., Berhaut, J., Payan, P. (1994): Primary culture of gill epithelial cells from the sea bass Dicentrarchus labrux. In Vitro Cell Dev Biol Anim, 30, 41-49.10.1007/BF026314178193773
Search in Google Scholar Back to article
Babich, H., Borenfreund, E. (1987): Cultured fish cells for the ecotoxicity testing of aquatic pollutants. Toxicity Assessment, 2, 2, 119-33.10.1002/tox.2540020202
Search in Google Scholar Back to article
Bakshi, A., Panigrahi, A. K. (2018): A comprehensive review on chromium induced alterations in fresh water Fishes. Toxicol Rep, 5, 440-447.10.1016/j.toxrep.2018.03.007597740829854615
Search in Google Scholar Back to article
Chaudhary, D. K., Sood, N., Rathore, G., Pradhan, P. K., Punia, P., Agarwal, N. K., Jena, J. K. (2014): Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822). Aquacult Res, 45, 299-311.10.1111/j.1365-2109.2012.03227.x
Search in Google Scholar Back to article
Dewan, S., Singh, Y., Bhatt, J. P. (2016): Proteome adjustments post gradual hypoxic stress in Barilius bendelisis: insights into adaptive strategies of a hill stream cyprinid. Current Proteomics, 13, 1, 20-30.10.2174/1570164613666160413122431
Search in Google Scholar Back to article
Di, W., Xia, L., Yanjie, Q., Liwen, B., Shijia, Z. (2017): Toxic effects of potassium dichromate on loach fin cell lines with different ploidies in vitro. J Fish China, 24, 1, 173-179.
Search in Google Scholar Back to article
Fan, T. J., Ren, B. X., Geng, X. F., Yu, Q. T., Wang, L. Y. (2010): Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus. Cytotechnology, 62, 3, 217-223.10.1007/s10616-010-9281-1293291020502962
Search in Google Scholar Back to article
Freshney, R. I. (2005): Culture of Animal Cells - A Manual of Basic Techniques. Wiley-Liss, New York, NY, USA, pp. 641.10.1002/9780471747598
Search in Google Scholar Back to article
Gjessing, M. C., Aamelfot, M., Batts, W. N., Benestad, S. L., Dale, O. B., Thoen, E. (2018): Development and characterization of two cell lines from gills of Atlantic salmon. Plos One, 13, 2: e0191792.10.1371/journal.pone.0191792581258629444101
Search in Google Scholar Back to article
Goswami, M., Sharma, B. S., Bahugun, S. N., Nagpure, N. S., Lakra, W. S. (2013): A SRCF cell line from snowtrout, Schizothorax richardsonii: Development and Characterization. Tissue Cell, 45, 219-226.10.1016/j.tice.2013.02.00223545061
Search in Google Scholar Back to article
Hamilton, F. (1822): An account of the fishes found in the river Ganges and its branches. Printed for a constable and company, Edinburgh & London, pp 405.10.5962/bhl.title.59540
Search in Google Scholar Back to article
Hightower, L. H., Renfro, J. L. (1988): Recent applications of fish cell culture to biomedical research. J Exp Zool, 248, 290-302.10.1002/jez.14024803073062124
Search in Google Scholar Back to article
IUCN Bangladesh (2010): Red book of threatened species of Bangladesh. IUCN-The world conservation union. Xii+, 116.
Search in Google Scholar Back to article
Ku, C. C., Teng, Y. C., Wang, C. S., Lu, C. H. (2009): Establishment and characterization of three cell lines derived from the rockfish grouper Epinephelus quoyanus: use for transgenic studies and cytotoxicity testing. Aquac, 294, 147-151.10.1016/j.aquaculture.2009.05.010
Search in Google Scholar Back to article
Lakra, W. S., Goswami, M., Swaminathan, T. R., Rathore, G. (2010): Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn). Biol Res, 43, 385-392.10.4067/S0716-97602010000400002
Search in Google Scholar Back to article
Lakra, W. S., Singh, Goswami, M., Gopalakrishnan, A., Lal, K. K., Mohindra, V., Sarkar, U. K., Punia, P., Singh, K. V,, Bhatt, J. P,, Ayyappan, S. (2016); DNA barcoding of Indian freshwater fishes. Mitochondrial DNA A, 27, 6, 4510-4517.10.3109/19401736.2015.110154026703324
Search in Google Scholar Back to article
Lakra, W. S., Swaminathan, T. R., Joy, K. P. (2011): Development, characterization, conservation and storage of fish cell lines: a review. Fish Physiol Biochem, 37, 1-20.10.1007/s10695-010-9411-x20607393
Search in Google Scholar Back to article
Ma, J., Sun, S., Zeng, L., Lu, Y. (2013): Establishment, characterization and viral susceptibility of two cell lines derived from leopard wrasse Macropharyngodon geoffroy. J Fish Biol, 83, 3, 560-573.10.1111/jfb.1219323991874
Search in Google Scholar Back to article
Majeed, S. A., Nambi, K. S., Taju, G., Hameed, A. S. (2015): Isolation, propagation, characterization, cryopreservation, and application of novel cardiovascular endothelial cell line from Channa striatus (Bloch, 1793). Cell Biochem Biophys 1, 71, 2, 601-16.10.1007/s12013-014-0240-x25194832
Search in Google Scholar Back to article
Marshall, E. (2005): Will DNA bar codes breathe life into classification? Science, 307, 1037.10.1126/science.307.5712.103715718446
Search in Google Scholar Back to article
Masuda, A., Ohtsuka, K., Matsuyama, M. (1990): Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus. In Vitro Cell Dev Biol Anim, 26, 713-721.10.1007/BF026244282384449
Search in Google Scholar Back to article
Mir, J. I., Patiyal, R. S., Sharma, N. K. (2015): Analysis of length-weight relationship of sympatric hill stream teleosts Barilius bendelisis (Hamilton, 1807) and Barilius vagra (Hamilton, 1822) from Garhwal Himalaya, India. J Appl Ichthyol, 31, 4, 771-772.10.1111/jai.12688
Search in Google Scholar Back to article
Mishra, AK, Lakra W. S., Bhatt, J. P., Goswami, M., Nagpure, N. S. (2012): Genetic characterization of two hill stream fish species Barilius bendelisis (Ham.1807) and Barilius barna (Ham.1822) using RAPD markers. Mol Biol Rep, 39, 10167-10172.10.1007/s11033-012-1890-723001325
Search in Google Scholar Back to article
Oo, W. (2002) Inland fisheries of the Union of Myanmar. In: Petr T, Swar DB (Eds.), Cold Water Fisheries in the Trans-Himalayan Countries. FAO Fisheries Technical Paper 431. http://www.fao.org/3/y3994e/y3994e09.htm
Search in Google Scholar Back to article
Palumbi, S., Martin, A., Romano, S., McMillan, W. O., Stice, L., Grabowski, G. (1991): The Simple Fool’s Guide to PCR. University of Hawaii, Honolulu, HI, USA. https://palumbilab.stanford.edu/SimpleFoolsMaster.pdf
Search in Google Scholar Back to article
Revest, J. M., Ravinder, Suniara, K., Kerr, K., John, Owen., J. J., Dickson, C. (2001): Development of the thymus requires signaling through the fibroblast growth factor receptor R2-IIIb. J Immunol, 167, 4, 1954-1961.10.4049/jimmunol.167.4.195411489975
Search in Google Scholar Back to article
Rodriguez, S. J. S., Gonzalez, C., Monras, M., Romero, A., Ballesteros, N., Enriquez, R., Perez-Prieto, S. (2014): Establishment and characterization of a new cell line SSP-9 derived from Atlantic salmon Salmo salar that expresses type I ifn. J Fish Biol, 85, 1526-1545.10.1111/jfb.1250325230295
Search in Google Scholar Back to article
Ryan, L. A., Seymour, C. B., Mehlenbacher, ONA., Mothersill, C. E. (2008): Radiation-induced adaptive response in fish cell lines. J Environ Radioact, 99, 739-747.10.1016/j.jenvrad.2007.10.00118054128
Search in Google Scholar Back to article
Sahoo, P. K., Saikia, S. K., Das. D. K. (2009): Natural food resources and niche breadth of Barilius bendelisis (Hamilton) (Pisces, Cyprinidae) in river Dikrong, an upland riverine ecosystem in India. Pan-Am j aquat, 4, 1, 12-16.
Search in Google Scholar Back to article
Sharma, N. K., Akhtar, M. S., Pandey, N., Singh, R., Singh, A. K. (2015): Seasonal variation in thermal tolerance, oxygen consumption, antioxidative enzymes and non-specific immune indices of Indian hill trout, Barilius bendelisis (Hamilton, 1807) from central Himalaya. India. J Therm Biol, 52166-52176.10.1016/j.jtherbio.2015.07.00526267511
Search in Google Scholar Back to article
Singh, A. K., Kumara, R., Mishra, A. K, Singh, M., Baisvar, V. S., Chauhan, U. K., Kushwaha, B., Nagpure, N. S. (2016): Authentication of five Barilius species from Indian waters using DNA barcoding. Russ J Genet, 52, 8, 840-846.10.1134/S1022795416060119
Search in Google Scholar Back to article
Soni, P., Pradhan, P. K., Swaminathan, T. R., Sood, N. (2018): Development, characterization and application of a new epithelial cell line from caudal fin of Pangasianodon hypophthalmus (Sauvage 1878) Acta Trop, 182, 215-222.10.1016/j.actatropica.2018.03.01529545155
Search in Google Scholar Back to article
Sood, N., Chaudhary, D. K., Pradhan, P. K., Verma, D. K., Swaminathan, T. R., Kushwaha B., Punia, P., Jena, J. K. (2015): Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793) In Vitro Cell Dev Biol Anim, 51, 8, 787–796.10.1007/s11626-015-9891-125877070
Search in Google Scholar Back to article
Sukham, M. S., Chingakham, B., Thoidingjam, L., Waikhom, G., Kumar, R., Kushwaha, B. (2013): A note on karyotype structure in Barilius bendelisis (Hamilton, 1807) (Cypriniformes: Cyprinidae) from Northeast India. Int J Fish Aquac, 3, 3, 42-46.
Search in Google Scholar Back to article
Swaminathan, T. R., Lakra, W.S., Gopalakrishnan, A., Basheer, V. S., Kushwaha, B., Sajeela. K. A. (2012): Development and characterization of a fibroblastic-like cell line from caudal fin of the red-line torpedo, Puntius denisonii (Day) (Teleostei: Cyprinidae). Aquacult Res, 43: 498–508.10.1111/j.1365-2109.2011.02854.x
Search in Google Scholar Back to article
Talwar, P. K., Jhingran, A. G. (1991): Inland Fishes of India and Adjacent Countries. Oxford and IBH Publishing Co, New Delhi.
Search in Google Scholar Back to article
Tan, F., Wang, M., Wang, W., Aguirre, A. A., Lu, Y. (2010): Validation of an in vitro cytotoxicity test for four heavy metals using cell lines derived from a green sea turtle (Chelonia mydas). Cell Biol Toxicol, 26, 255-263.10.1007/s10565-009-9130-119629729
Search in Google Scholar Back to article
Tchounwou, P. B., Yedjou, C. G., Patlolla, A. K., Sutton, D. J. (2012): Heavy metal toxicity and the environment. In Molecular, clinical and environmental toxicology. Springer, Basel 133-164.10.1007/978-3-7643-8340-4_6
Search in Google Scholar Back to article
Tong, J., Wang, Y., Lu, Y. (2016): In vitro evaluation of inorganic and methyl mercury mediated cytotoxic effect on neural cells derived from different animal species. J Environ Sci, 41, 138-145.10.1016/j.jes.2015.04.036
Search in Google Scholar Back to article
Tong, S. L., Lee, H., Miao, H. Z. (1997): The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus. Aquac, 156, 327–333.10.1016/S0044-8486(97)00070-7
Search in Google Scholar Back to article
Vishwanath, W. (2010): Barilius bendelisis. The IUCN Red List of Threatened Species 2010:e.T166594A6243552. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2010-4.RLTS.T166594A6243552.en.10.2305/IUCN.UK.2010-4.RLTS.T166594A6243552.en
Search in Google Scholar Back to article
Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., Hebert, P. D. N. (2005): DNA barcoding Australia’s fish species. Philos Trans R Soc Lond B Biol Sci, 360, 1847-1857.10.1098/rstb.2005.1716160923216214743
Search in Google Scholar Back to article

Development of Caudal Fin Cell Line from Hill Trout Barilius Bendelisis (Hamilton, 1807) for Cytotoxicity and Transfection Studies

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