A New Species of Mullet Chelon caeruleum (Family: Mugilidae) with Description of its Genetic Relationship to Some Mugilids

Deef, Lamiaa Elsayed Mokhtar

doi:10.2478/cjf-2018-0014

Abstract

Chelon caeruleum sp. nov. is described as a new species of Chelon encountered in the Rashid coastal region of the Mediterranean Sea, Egypt. With this finding, the new species is the sixth member of the genus Chelon. Chelon caeruleum sp. nov. is distinguished from its congeners by the following characters: colouration of body is dark bluish grey dorsally and the sides are blue; head length 20.5 to 21.6% SL; head width 13 to 13.5% SL; pre-first dorsal fin length 50% SL; eye diameter 4.6 to 5.2% SL; postorbital length 10.7 to 11.1% SL; unbranched pyloric caeca are 2 short and 4 long; a pair of long (one longer than the other), spine-like neural postzygapophyse on the second vertebra. DNA of Chelon caeruleum sp. nov. and five mullet species (Mugil cephalus, Liza carinata, Liza ramada, Liza aurata, and Chelon labrosus) was extracted then amplified using polymerase chain reaction (PCR) and sequenced. The mtDNA cytochrome oxidase subunit I sequence analysis confirmed that Chelon caeruleum sp. nov. is distinct from other congeners of Chelon and it is a different species of Chelon which is new to science.

References

Caldara, F., Bargelloni, L., Ostellari, L., Penzo, E., Colombo, L., Patarnello, T. (1996): Molecular phylogeny of grey mullets based on mitochondrial DNA sequence analysis: evidence of a differential rate of evolution at the intrafamily level. Molecular Phylogenetics and Evolution, 6, 3, 416–424.10.1006/mpev.1996.00908975696
Search in Google Scholar Back to article
Durand, J.D., Hubert, N., Shen, K.N., Borsa, P. (2017) DNA barcoding grey mullets. Reviews in Fish Biology and Fisheries, 27, 233–243.10.1007/s11160-016-9457-7
Search in Google Scholar Back to article
Fraga, E., Schneider, H., Nirchio, M., Santa-Brigida, E., RodriguesFilho, L.F., Sampaio, I. (2007): Molecular phylogenetic analyses of mullets (Mugilidae, Mugiliformes) based on two mitochondrial genes. Journal of Applied Ichthyology, 23, 5, 598-604.10.1111/j.1439-0426.2007.00911.x
Search in Google Scholar Back to article
Harrison, I.J., Nirchio, M., Oliveira, C., Ron, E., Gaviria, J. (2007): A new species of mullet (Teleostei: Mugilidae) from Venezuela, with a discussion on the taxonomy of Mugil gaimardianus. Journal of Fish Biology, 71, 76-97.10.1111/j.1095-8649.2007.01520.x
Search in Google Scholar Back to article
Heras, S., Roldan, M.I., Castro, M.G. (2009): Molecular phylogeny of Mugilidae fishes revised. Reviews in Fish Biology and Fisheries, 19, 2, 217-231.10.1007/s11160-008-9100-3
Search in Google Scholar Back to article
Hillis, D.M., Bull, J.J. (1993): An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 2, 182-192.10.1093/sysbio/42.2.182
Search in Google Scholar Back to article
Liu, J.Y., Brown, C.L., Yang, T.B. (2010): Phylogenetic relationships of mullets (Mugilidae) in China Seas based on partial sequences of two mitochondrial genes. Biochemical Systematics and Ecology, 38, 4, 647-655.10.1016/j.bse.2010.01.007
Search in Google Scholar Back to article
Martin, A.W. (1995): Mitochondrial DNA sequence evolution in sharks: rates, patterns, and phylogenetic inferences. Molecular Biology and Evolution, 12, 6, 1114-1123.
Search in Google Scholar Back to article
Murgia, R., Tola, G., Archer, S.N., Vallerga, S., Hirano, J. (2002): Genetic identification of grey mullet species (Mugilidae) by analysis of mitochondrial DNA sequence: application to identify the origin of processed ovary products (bottarga). Marine Biotechnology, 4, 2, 119–126.10.1007/s10126-001-0074-614961270
Search in Google Scholar Back to article
Nelson, J.S. (2006): Fishes of the World. John Wiley and Sons, New York.
Search in Google Scholar Back to article
Papasotiropoulos, V., Klossa-Kilia, E., Kilias, G., Alahiotis, S. (2001): Genetic divergence and phylogenetic relationships in grey mullets (Teleostei: Mugilidae) using allozyme data. Biochemical Genetics, 39, 5, 155–168.10.1023/A:1010289224573
Search in Google Scholar Back to article
Papasotiropoulos, V., Klossa-Kilia, E., Kilias, G., Alahiotis, S. (2002): Genetic divergence and phylogenetic relationships in grey mullets (Teleostei: Mugilidae) based on PCR–RFLP analysis of mtDNA segments. Biochemical Genetics, 40, 3, 71–86.10.1023/A:1015162304934
Search in Google Scholar Back to article
Papasotiropoulos, V., Klossa-Kilia, E., Alahiotis, S., Kilias, G. (2007): Molecular phylogeny of grey mullets (Teleostei: Mugilidae) in Greece: evidence from sequence analysis of mtDNA segments. Biochemical Genetics, 45, 7, 623-636.10.1007/s10528-007-9101-217588202
Search in Google Scholar Back to article
Rossi, A.R., Ungaro, A., De Innocentiis, S., Crosetti, D., Sola, L. (2004): Phylogenetic analysis of Mediterranean Mugilids by allozymes and 16S mtrRNA genes investigation: are the Mediterranean species of Liza monophyletic? Biochemical Genetics, 42, 9, 301–315.10.1023/B:BIGI.0000039806.12756.81
Search in Google Scholar Back to article
Schultz, L.P. (1946): A revision of the genera of mullets, fishes of the family Mugilidae, with descriptions of 3 new genera. Proceedings of the United States National Museum, 96, 377-395.10.5479/si.00963801.96-3204.377
Search in Google Scholar Back to article
Semina, A.V., Polyakova, N.E., Makhotkin, M.A., Brykov, V.A. (2007): Mitochondrial DNA divergence and phylogenetic relationships in mullets (Pisces: Mugilidae) of the Sea of Japan and the Sea of Azov revealed by PCR–RFLP-analysis. Russian Journal of Marine Biology, 33, 3, 187-192.10.1134/S1063074007030078
Search in Google Scholar Back to article
Senou, H., Randall, J.E., Okiyama, M. (1996): Chelon persicus, a New Species of Mullet (Perciforrnes: Mugilidae) from the Persian Gulf. Bulletin of the Kanagawa Prefectural Museum Natural Science, 25, 71-78.
Search in Google Scholar Back to article
Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013): MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729.10.1093/molbev/mst197384031224132122
Search in Google Scholar Back to article
Thomson, J.M. (1954): The Mugilidae of Australia and adjacent seas. Australian Journal of Marine and Freshwater Research, 5, 1, 70-131, pls. 1-2.10.1071/MF9540070
Search in Google Scholar Back to article
Thomson, J.M. (1997): The Mugilidae of the World. Memoirs of the Queensland Museum, 43, 457-562.
Search in Google Scholar Back to article
Trewavas, E., Ingham, S. E. (1972): A key to the species of Mugilidae (Pisces) in the northeastern Atlantic and Mediterranean, with explanatory notes. Journal of Zoology London, 167, 15-29.10.1111/j.1469-7998.1972.tb01720.x
Search in Google Scholar Back to article
Turan, C., Gürlek, M., Ergüden, D., Yağlıoğlu, D., Öztürk, B. (2011): Systematic Status of Nine Mullet Species (Mugilidae) in the Mediterranean Sea. Turkish Journal of Fisheries and Aquatic Sciences, 11, 315-321.
Search in Google Scholar Back to article
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N. (2005): DNA barcoding Australia›s fish species. Philosophical Transactions of the Royal Society of London. Series B, Biological sciences, 360, 1847–1857.10.1098/rstb.2005.1716160923216214743
Search in Google Scholar Back to article

A New Species of Mullet Chelon caeruleum (Family: Mugilidae) with Description of its Genetic Relationship to Some Mugilids

Abstract

Paradigm

My account