Skip to main content

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates Cover

.blurhash-client-img { display: none !important; }

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Current Issues in Pharmacy and Medical Sciences

Volume 35 (2022): Issue 2 (August 2022)

By: Adam Chomicki and Tadeusz H. Dzido

Open Access

|Sep 2022

Abstract

Investigations into separation selectivity of High-Performance Thin-layer chromatography (HPTLC) of dansyl (DNS) derivatives of amino acids in silica gel (Silica gel 60 F_254s plates) and silanized silica gel (RP-18 W plates) systems are presented. The results have been obtained for mobile phases containing different concentrations of acetonitrile (ACN) in formic acid (FA) water solution (final concentration of FA in the mobile phase was equal to 265 mmol/dm³). The data obtained show differences in separation selectivity of the solutes between employment of HPTLC silica gel and RP-18 W systems.

References

1. Roda JM, Pascual JM, Carceller F, González-Llanos F, Pérez-Higueras A, Solivera J, et al. Nonhistological diagnosis of human cerebral tumors by 1H magnetic resonance spectroscopy and amino acid analysis. Clin Cancer Res. 2000;6:3983-93.
Search in Google Scholar Back to article
2. Pisters PWT, Pearlstone DB. Protein and amino acid metabolism in cancer cachexia: investigative techniques and therapeutic interventions. Crit Rev Clin Lab Sci. 1993;3:223-72.10.3109/10408369309084669
Open DOI Search in Google Scholar Back to article
3. Herbet M, Natorska Chomicka D, Korga A, Ostrowska M, Izdebska M, Gawrońska Grzywacz M, et al. Altered expression of genes involved in brain energy metabolism as adaptive responses in rats exposed to chronic variable stress; changes in cortical level of glucogenic and neuroactive amino acids. Mol Med Rep. 2019;19:2386-96.10.3892/mmr.2019.9865
Open DOI Search in Google Scholar Back to article
4. Tajiri K, Shimizu Y. Branched-chain amino acids in liver diseases. World J Gastroenterol. 2013;43:7620-9.10.3748/wjg.v19.i43.7620
Open DOI Search in Google Scholar Back to article
5. Weiss R, Kim K. Metabolomics in the study of kidney diseases. Nat Rev Nephrol. 2012;8:22-33.10.1038/nrneph.2011.152
Open DOI Search in Google Scholar Back to article
6. Shockcor JP, Holmes E. Metabonomic applications in toxicity screening and disease diagnosis. Curr Top Med Chem. 2002;2:35-51.10.2174/1568026023394498
Open DOI Search in Google Scholar Back to article
7. Kupec J, Turina S. The determination of some amino acids by conventional and hot plate chromatography. Chromatographia. 1977;10:157-9.10.1007/BF02297870
Open DOI Search in Google Scholar Back to article
8. Wollenweber P. Separation of amino acids by thin-layer chromatography on cellulose. J Chromatogr. 1962;9:369-71.10.1016/S0021-9673(00)80801-8
Open DOI Search in Google Scholar Back to article
9. Haworth C, Heathcote JG. The direct determination of amino acids on thin-layer chromatograms by densitometry. Biochem J. 1969; 114:667-8.10.1042/bj114066711849445820651
Open DOI Search in Google Scholar Open in PubMed Back to article
10. Bhushan R, Ali I. TLC resolution of amino Acids in a new solvent and effect of alkaline earth metals. J Liq Chromatogr. 1987;10:3647-52.10.1080/01483918708077820
Open DOI Search in Google Scholar Back to article
11. Sleckman BP, Sherma J. A Comparison of amino acid separations on silica gel, cellulose, and ion exchange thin layers. J Liq Chromatogr. 1982;5:1051-68.10.1080/01483918208067568
Open DOI Search in Google Scholar Back to article
12. Vasta JD, Cicchi M, Sherma J, Fried B. Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures. Acta Chromatogr. 2009;21:29-38.10.1556/AChrom.21.2009.1.3
Open DOI Search in Google Scholar Back to article
13. Sherma J, Sleckman BP, Armstrong DW. Chromatography of amino acids on reversed phase thin layer plates. J Liq Chromatogr. 1983;6:95-108.10.1080/01483918308066873
Open DOI Search in Google Scholar Back to article
14. Baranowska I, Kozłowska M. TLC separation and derivative spectrophotometry of some amino acids. Talanta. 1995;42:1553-7.10.1016/0039-9140(95)01569-W
Open DOI Search in Google Scholar Back to article
15. Polak B, Hałka A, Dzido TH. Pressurized planar electrochromato-graphic separation of the enantiomers of tryptophan and valine. J Planar Chromatogr. 2008;21:33-7.10.1556/JPC.21.2008.1.5
Open DOI Search in Google Scholar Back to article
16. Polak B, Wojtanowski KK, Ślązak P, Dzido TH. Separation of some aromatic amino acid enantiomers with pressurized planar electrochromatography and TLC. Chromatographia. 2011;73:339-45.10.1007/s10337-010-1888-z
Open DOI Search in Google Scholar Back to article
17. Polak B, Balasa K, Dzido TH. Separation of amino acid 2,4-Dinitrophenyl-5-L-valine amide diastereomeric derivatives with high-performance planar chromatography and pressurized planar electrochromatography. J Planar Chromatogr. 2013;28:180-9.10.1556/JPC.26.2013.2.13
Open DOI Search in Google Scholar Back to article
18. Polak B, Maruszak A, Płocharz PW. High-performance thin-layer chromatography and pressurized planar electrochromatography of some diastereomeric amino acid derivatives in reversed -phase system with carboxylic acid mobile phase buffers. J Planar Chromatogr. 2016;29:22-9.10.1556/1006.2016.29.1.3
Open DOI Search in Google Scholar Back to article
19. Polak B, Garbacz P. β- cyclodextrin as the mobile phase component for separation of some DNS-amino acid enantiomers with HPTLC and PPEC. Curr Anal Chem. 2015;11:68-77.10.2174/1573411010666141127211110
Open DOI Search in Google Scholar Back to article
20. Chomicki A, Kloc K, Dzido TH. Two-dimensional separation of some amino acids by HPTLC and pressurized planar electrochromatography. J Planar Chromatogr. 2011;24:16-9.10.1556/JPC.24.2011.1.1
Open DOI Search in Google Scholar Back to article
21. Chomicki A, Dzido TH, Materna-Witkowska E, Klimek-Turek A. Thin-layer chromatography and pressurized planar electrochromatography of amino acids in systems with silica gel and water mobile phase. J Planar Chromatogr. 2016;29:30-7.10.1556/1006.2016.29.1.4
Open DOI Search in Google Scholar Back to article
22. Fager RS, Kutina CB. Resolution of dansyl leucine and dansyl isoleucine. J Chromatogr. 1973;76:268-9.10.1016/S0021-9673(01)97808-2
Open DOI Search in Google Scholar Back to article
23. Bhushan R, Reddy GP. Some solvent systems for the resolution of dansyl amino acids by silicagel thin layer chromatography. J Li Chromatogr. 1988;11:3163-70.10.1080/01483918808076786
Open DOI Search in Google Scholar Back to article
24. Wood KR, Wang KT. Separation of dansyl-amino acids by polyamide layer chromatography. Biochem Biophys Acta. 1967;133:369-70.10.1016/0005-2795(67)90078-5
Open DOI Search in Google Scholar Back to article
25. Crowshaw K, Jessup SJ, Ramwell PW. Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex. Biochem J. 1967;103:79-85.10.1042/bj1030079
Open DOI Search in Google Scholar Back to article
26. Biou D, Queyrel N, Visseaux MN, Collignon I, Pays M. Separation and identification of dansylated human serum and urinary amino acids by two-dimensional thin-layer chromatography : Application to aminoacidopathies. J Chromatogr. 1981;226:477-83.10.1016/S0378-4347(00)86084-3
Open DOI Search in Google Scholar Back to article
27. Wesenberg JC, Jacqueline EJ. Screening for amino acid disorders by thin-layer chromatography of the dansyl amino acids. Microchim Acta. 1980;74:1-7.10.1007/BF01196480
Open DOI Search in Google Scholar Back to article
28. Macek K, Deyl Z, Smrz M. Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel. J Chromatogr A. 1980;193:521-426.10.1016/S0021-9673(00)87742-0
Open DOI Search in Google Scholar Back to article
29. Lepri L, Coas V, Desideri PG. et al. Reversed phase planar chromatography of dansyl DL amino acids with bovine serum albumin in the mobile phase. Chromatographia. 1993;36:297-301.10.1007/BF02263881
Open DOI Search in Google Scholar Back to article
30. Sinibaldi M, Messina A, Girelli AM. Separation of dansylamino acid enantiomers by thin-layer chromatography. Analyst. 1988;113:1245-7.10.1039/an9881301245
Open DOI Search in Google Scholar Back to article
31. Weinstein S. Resolution of optical isomers by thin layer chromatography. Tetrahedron Lett. 1984;25:985-6.10.1016/S0040-4039(01)80080-0
Open DOI Search in Google Scholar Back to article
32. LeFevre JW, Dodsworth DW. Complete analysis of a biologically active tetrapeptide: A project utilizing thin-layer chromatography and mass spectrometry. J Chem Educ. 2000;77:503-4.10.1021/ed077p503
Open DOI Search in Google Scholar Back to article
33. LeFevre JW, Gublo EJ, Botting C, Wall R, Nigro A, Pham MLT, Ganci G. Qualitative reversed-phase thin-layer chromatographic analysisof the stereochemistry of D- and L-alpha-amino acids in small peptides. J Planar Chromatogr. 2000;13:160-5.
Search in Google Scholar Back to article

Articles in this issue

DOI: https://doi.org/10.2478/cipms-2022-0011 | Journal eISSN: 2300-6676 | Journal ISSN: 2084-980X

Journal RSS Feed

Language: English

Page range: 58 - 61

Submitted on: Jan 19, 2022

|

Accepted on: Jun 24, 2022

|

Published online: Sep 2, 2022

Published in print: Aug 1, 2022

Published by: Sciendo

In partnership with: Paradigm Publishing Services

Keywords:

thin layer chromatography,

dansyl derivatives

Related subjects:

Clinical medicine,

Pharmacology, toxicology

© 2022 Adam Chomicki, Tadeusz H. Dzido, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 35 (2022): Issue 2 (August 2022)

Previous article