Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Yulia Bazarnova; Tatiana Bolotnikova; Ekaterina Aronova

doi:10.2478/cipms-2019-0024

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Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Current Issues in Pharmacy and Medical Sciences

Volume 32 (2019): Issue 3 (September 2019)

By: Yulia Bazarnova, Tatiana Bolotnikova and Ekaterina Aronova

Open Access

|Sep 2019

Abstract

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.

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Articles in this issue

DOI: https://doi.org/10.2478/cipms-2019-0024 | Journal eISSN: 2300-6676 | Journal ISSN: 2084-980X

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Language: English

Page range: 130 - 133

Submitted on: Nov 6, 2018

Accepted on: Feb 8, 2019

Published online: Sep 28, 2019

Published in print: Sep 1, 2019

Published by: Sciendo

In partnership with: Paradigm Publishing Services

Keywords:

candidate cholera vaccine,

hybrid tbf gene,

Sanger sequencing,

protein design,

TBF recombinant protein,

immunodominant epitopes,

Vibrio choleraе

Related subjects:

Medicine,

Clinical medicine,

Pharmacology, toxicology

© 2019 Yulia Bazarnova, Tatiana Bolotnikova, Ekaterina Aronova, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 32 (2019): Issue 3 (September 2019)