Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

Andraszek, Katarzyna; Banaszewska, Dorota; Szostek, Małgorzata; Wójcik, Ewa; Czubaszek, Magdalena; Walczak-Jędrzejowska, Renata

doi:10.2478/bvip-2014-0074

Abstract

The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate - AgNO3. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO3 staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.

References

1. Andraszek K., Smalec E.: The use of silver nitrate for the identification of spermatozoon structure in selected mammals. Can J Anim Sci 2011, 91, 239-246.10.4141/cjas10052
Search in Google Scholar
2. Andraszek K., Gryzińska M. Knaga S., Wójcik E., Smalec E.: Number and size of nucleoli in the spermatocytes of chicken and Japanese quail. Folia Biol (Krakow) 2012, 60, 121-127.10.3409/fb60_3-4.121-12723342905
Search in Google Scholar
3. Bielańska-Osuchowska Z., Sysa P.: Ultrastructure of the mammalian sperm. In: Cell ultrastructure and function. Mechanisms of regulation, edited by Łukaszczyk A., Bilińska B., Kawiak J., Bielańska-Osuchowska Z., PWN SA. Warszawa, Poland, 1998, pp. 76-94.
Search in Google Scholar
4. Bongso T.A.: Comparative silver staining patterns of water buffalo, goat and pig spermatozoa. Arch Androl 1983, 11, 13 -17.10.3109/014850183089874536625746
Search in Google Scholar
5. Carrell D.T., Hammoud S.S.: The human sperm epigenome and its potential role in embryonic development. Mol Hum Reprod 2010, 16, 37-47.10.1093/molehr/gap09019906823
Search in Google Scholar
6. Cohen J., Matter H., Wright G., Kort H., Massey J., Mitchell D.: Partial zone dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum Reprod 1989, 4, 435-442.10.1093/oxfordjournals.humrep.a1369232745674
Search in Google Scholar
7. Dehghani H., Dellaire G., Bazett-Jones D.P.: Organization of chromatin in the interphase mammalian cell. Micron 2005, 36, 95-108.10.1016/j.micron.2004.10.00315629642
Search in Google Scholar
8. Esteso M.C., Fernández-Santos M.R., Soler A.J., Montoro V., Quintero-Moreno A., Garde J.J.: The effects of cryopreservation on the morphometric dimensions of Iberian red deer (Cervus elaphus hispanicus) epididymal sperm heads. Reprod Domest Anim 2006, 41, 241 -246.10.1111/j.1439-0531.2006.00676.x16689889
Search in Google Scholar
9. Evans E.P., Breckon G., Ford C.E.: An air-drying method for meiotic preparation from mammalian testes. Cytogenetics 1964, 3, 295-298.10.1159/00012981814248459
Search in Google Scholar
10. Evenson D.P., Jost L.K., Marshall D., Zinaman M.J., Clegg E., Purvis K., de Angelis P., Claussen O.P.: Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 1999, 14, 1039-1049.10.1093/humrep/14.4.103910221239
Search in Google Scholar
11. Guzick D.S., Overstreet J.W., Factor-Litvak P., Brazil C.K., Nakajima S.T., Coutifarls C., Carson S.A., Cisneros P., Steinkampf M.P., Hill J.A., Xu D., Vogel D.L.: Sperm morphology, motility, and concentration in fertile and infertile men. N Eng J Med 2001, 345, 1388-1393.10.1056/NEJMoa00300511794171
Search in Google Scholar
12. Henkel R., Schreiber G., Sturmhoefel A., Hipler U.C., Zermann D.H., Menkveld R.: Comparison of three staining methods for the morphological evaluation of human spermatozoa. Fertil Steril 2008, 89, 449-455.10.1016/j.fertnstert.2007.03.02717601601
Search in Google Scholar
13. Hidalgo M., Rodriguez I., Dorado J.: Influence of staining and sampling procedures on goat sperm morphometry using the Sperm Class Analyzer. Theriogenology 2006, 66, 996-1003.10.1016/j.theriogenology.2006.02.03916620924
Search in Google Scholar
14. Howell W.M., Black D.A.: Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer. a 1 -step method. Experentia 1980, 36, 1014-1015.10.1007/BF019538556160049
Search in Google Scholar
15. Iranpour F.G., Nasr-Esfahani M.H., Valojerdi M.R., al-Taraihi T.M.: Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet 2000, 17, 60-66.10.1023/A:1009406231811
Search in Google Scholar
16. Jenkins T.G., Emery B.R., Carrell D.T.: Assays used in the study of sperm nuclear proteins. In: Sperm Chromatin - Biological and Clinical Applications in Male Infertility and Assisted Reproduction, edited by Springer Science+Business Media, London, 2011, pp. 233-241.
Search in Google Scholar
17. Kazerooni T., Asadi N., Jadid L., Kazerooni M., Ghanadi A., Ghaffarpasand F., Kazerooni Y., Zolghadr J.: Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 2009, 26, 591-596.10.1007/s10815-009-9361-3279955819894107
Search in Google Scholar
18. Kim H.S., Kang M.J., Kim S.A., Oh S.K., Kim H., Ku S.Y., Kim S.H., Moon S.Y., Choi Y.M.: The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis. Clin Exp Reprod Med 2013, 40, 23-28.10.5653/cerm.2013.40.1.23363028923614112
Search in Google Scholar
19. Maree L., du Plessis S.S., Menkveld R., van der Horst G.: Morphometric dimensions of the human sperm head depend on the staining method used. Hum Reprod 2010, 25, 1369-1382.10.1093/humrep/deq07520400771
Search in Google Scholar
20. Menkveld R., El-Garem Y., Schill W.B., Henkel R.: Relationship between human sperm morphology and acrosomal function. J Assist Reprod Genet 2003, 20, 432-438.10.1023/A:1026288710638
Search in Google Scholar
21. Molina J., Castilla J.A., Castano J.L., Fontes J., Mendoza N., Martinez L.: Chromatin status in human ejaculated spermatozoa from infertile patients and relationship to seminal parameters. Hum Reprod 2001, 16, 534-539.10.1093/humrep/16.3.53411228225
Search in Google Scholar
22. Oral E., Yetis O., Elibol F., Senol H., Irez T., Aksu F.M.: Assessment of human sperm morphology by strict criteria, comparison of wet preparation versus stained with the modified Diff-Quik method. Arch Androl 2002, 48, 307-314.10.1080/0148501029003162812137592
Search in Google Scholar
23. Piasecka M.: Morphology and function of sperm mitochondria and male fertility. Part I. Normal morphology and function of sperm mid-piece. Post Biol Kom 2004, 31, 489-516.
Search in Google Scholar
24. Schulte R.T., Ohl D.A., Sigman M., Smith G.D.: Sperm DNA damage in male infertility, etiologies, assays, and outcomes. J Assist Reprod Genet 2010, 27, 3-12.10.1007/s10815-009-9359-x282662620012685
Search in Google Scholar
25. Spanò M., Cordelli E., Leter G., Lombardo F., Lenzi A., Gandini L.: Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. Mol Hum Reprod 1999, 5, 29-37.10.1093/molehr/5.1.2910050659
Search in Google Scholar
26. Sutovsky P., Manandhar G.: Mammalian spermatogenesis and sperm structure, anatomical and compartmental analysis. In: The sperm cell. Production, maturation, fertilization, regeneration, edited by Cambridge University Press, Cambridge, UK, 2006, pp. 1 -31.
Search in Google Scholar

Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species

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