MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms

Łosiewicz, Katarzyna; Chmielewska-Krzesińska, Małgorzata; Socha, Piotr; Jakimiuk, Anna; Wąsowicz, Krzysztof

doi:10.2478/bvip-2014-0068

Abstract

The expression of miRNA-21, miRNA-10b, and miRNA-34a in malignant and benign tumours and non-neoplastic lesions in canine mammary gland, using real-time PCR with TaqMan probes was determined. The expression in normal tissues was compared to neoplastic and non-neoplastic lesions using one-way ANOVA test. Significant changes in miRNA expression in neoplastic tissues, as compared to normal ones, were demonstrated. In all neoplastic tissues, the miRNA-21 expression increased while in non-neoplastic lesions slightly decreased in comparison to normal ones. MiRNA-10b expression in malignant and benign tumours increased in comparison to normal tissues and non-neoplastic lesions. MiRNA-34a expression profile in neoplastic and non-neoplastic tissues differed from other examined miRNAs (miRNA-21 and miRNA-10b). In all samples miRNA-34a expression level decreased in comparison to normal tissues.

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MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms

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