Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats

Hinako Sashida; Ryô Harasawa; Toshihiro Ichijo; Hiroshi Satoh; Kazuhisa Furuhama

doi:10.2478/bvip-2014-0058

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Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats

Journal of Veterinary Research

Volume 58 (2014): Issue 3 (September 2014)

By: Hinako Sashida, Ryô Harasawa, Toshihiro Ichijo, Hiroshi Satoh and Kazuhisa Furuhama

Open Access

|Oct 2014

Abstract

The presence of Mycoplasma haemomuris (haemoplasma) in blood samples collected from specific pathogen-free (SPF) laboratory rats bred in Japan was reported. Its presence was examined in Fischer 344, Sprague-Dawley (SD), and Wistar rat strains of both sexes by real-time PCR. All strains were positive for M. haemomuris infection. The 16S rRNA gene of M. haemomuris strain detected in the animals was amplified using end-point PCR. Only the entire nucleotide sequence of 16S rRNA gene of a mycoplasma strain detected in SD rats was determined and compared to those of other haemoplasmas. Our investigations suggest a wide M. haemomuris infection among the SPF rats purchased from commercial breeders in Japan.

References

1. Amici D., Murri O., Paparelli M, Tedeschi G.G.: Transmissione della Haemobartonella muris della madre al feto. Boll Soc Ital Biol Sper 1966, 42, 1501-1503.
Search in Google Scholar
2. Baker H.J., Cassell G.H., Lindsey J.R.: Research complications due to Haemobartonella and Eperythrozoon infections in experimental animals. Am J Pathol 1971, 64, 625-652.
Search in Google Scholar
3. Bartlett R., Pease P.: Latent Haemobartonella muris infection: its transmission and decline in an inbred, ectoparasite-free strain of Wistar rat. Parasitology 1975, 71, 35-40.10.1017/S0031182000053129
Search in Google Scholar
4. Crystal M.M.: The mechanism of transmission of Haemobartonella muris (Mayer) of rats by the spined rat louse, Polyplax spinulosa (Burmeister). J Parasitol 1958, 44, 603-606.10.2307/3274543
Search in Google Scholar
5. Kimura M.: A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980, 16, 111-120.10.1007/BF017315817463489
Search in Google Scholar
6. Kreier J.P., Hall L.: The relationship of parasitemia to the life span of erythrocytes of rats infected with Haemobartonella muris. J Infect Dis 1968, 118, 443-448.10.1093/infdis/118.5.4435701288
Search in Google Scholar
7. Messick J.B.: Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet Clin Pathol 2004, 33, 2-13.10.1111/j.1939-165X.2004.tb00342.x
Search in Google Scholar
8. Moore D.H., Arison R.N., Tanaka H., Hall W.T., Chanowitz M.: Identity of the filterable hemolytic anemia agent of Sacks with Haemobartonella muris. J Bacteriol 1965, 90, 1669-1674.10.1128/jb.90.6.1669-1674.19653158765854589
Search in Google Scholar
9. Nishizawa I., Sato M., Fujihara M., Sato S., Harasawa R.: Differential detection of hemotropic Mycoplasma species in cattle by melting curve analysis of PCR products. J Vet Med Sci 2010, 72, 1704. Rikihisa Y., Kawahara M., Wen B., Kociba G., Fuerst P., Kawamori F., Suto C., Shibata S., Futohashi M.: Western immunoblot analysis of Haemobartonella muris and comparison of 16S rRNA gene sequences of H. muris, H. felis, and Eperythrozoon suis. J Clin Microbiol 1997, 35, 823-829.10.1128/jcm.35.4.823-829.19972296839157135
Search in Google Scholar
10. Saitou N., Nei M.: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4, 406-425.
Search in Google Scholar
11. Thompson J.D., Higgins D.G., Gibson T.J.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22, 4673-4680.10.1093/nar/22.22.46733085177984417
Search in Google Scholar
12. Woods R., Parbhoo S.P.: Haemolytic anaemia in the Gunn rat following transplantation of isolated hepatocytes injected with Haemobartonella muris. Br J Exp Pathol 1981, 62, 8-12.
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/bvip-2014-0058 | Journal eISSN: 2450-8608

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Language: English

Page range: 375 - 378

Submitted on: Feb 13, 2014

Accepted on: Aug 6, 2014

Published on: Oct 1, 2014

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

rat,

specific pathogen free state,

Mycoplasma haemomuris

Related subjects:

Life sciences,

Molecular biology,

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2014 Hinako Sashida, Ryô Harasawa, Toshihiro Ichijo, Hiroshi Satoh, Kazuhisa Furuhama, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 58 (2014): Issue 3 (September 2014)