Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Nadăş, George Cosmin; Kalmár, Zsuzsa; Taulescu, Marian Aurel; Chirilă, Flore; Bouari, Cosmina Maria; Răpuntean, Sorin; Bolfa, Pompei; Fiţ, Nicodim Iosif

doi:10.2478/bvip-2014-0032

Abstract

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

References

1. Chen Y.C., Eisner J.D., Kattar M.M., Rassoulian-Barrett S.L., LaFe K., Yarfitz S.L., Limaye A.P., Cookson B.T.: Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA genes. J Clin Microbiol 2000, 38, 2302-2310.10.1128/JCM.38.6.2302-2310.2000
Search in Google Scholar
2. Coleman D.C., Moran G.P., McManus B.A., Sullivan D.J.: Mechanisms of antifungal drug resistance in Candida dubliniensis. Future Microbiol 2010, 5, 935-949.10.2217/fmb.10.51
Search in Google Scholar
3. Costa G.M., Paiva L.V., Figueiredo H.C., Figueira A.R., Pereira U.P., Silva N.: Population diversity of Staphylococcus aureus isolated from bovine mastitis in Brazilian dairy herds. Res Vet Sci 2012, 93, 733-735.10.1016/j.rvsc.2011.09.014
Search in Google Scholar
4. Fotedar R., Al-Hedaithy S.S.: Comparison of phospholipase and proteinase activity in Candida albicans and C. dubliniensis. Mycoses 2005, 48, 62-67.10.1111/j.1439-0507.2004.01057.x
Search in Google Scholar
5. Hatta D.J., Hall L., Fothergill A.W., Larone D.H., Wengenack N.L.: Multicenter evaluation of the new Vitek 2 advanced colorimetric yeast identification card. J Clin Microbiol 2007, 45, 1087-1092.10.1128/JCM.01754-06
Search in Google Scholar
6. Hospenthal D.R., Beckius M.L., Floyd K.L., Horvath L.L., Murray C.K.: Presumptive identification of Candida species other than C. albicans, C. krusei and C. tropicalis with the chromogenic medium CHROMagar Candida. Ann Clin Microb Antimicrob 2006, 5:1.10.1186/1476-0711-5-1
Search in Google Scholar
7. Lee L.H., Shien J.H., Shieh H.K.: Detection of avian reovirus RNA and comparison of a portion of genome segment S3 by polymerase chain reaction and restriction enzyme fragment length polymorphism. Res Vet Sci 1998, 65, 11-15.10.1016/S0034-5288(98)90020-0
Search in Google Scholar
8. Liguori G., Di Onofrio V., Gallé F., Lucariello A., Albano L., Catania M.R., Guida M.: Candida albicans identification: comparison among nine phenotypic systems and a multiplex PCR. J Prev Med Hyg 2010, 51, 121-124.
Search in Google Scholar
9. Liguori G., Galle F., Lucariello A., Di Onofrio V., Albano L., Mazzarella G., D’Amora M., Rossano F.: Comparison between multiplex PCR and phenotypic systems for Candida identification. New Microbiol 2010, 33, 63-67.
Search in Google Scholar
10. Madhavan P., Jamal F., Chong P.P., Ng K.P.: Identification of local clinical Candida isolates using CHROMagar Candida as a primary identification method for various Candida species. Trop Biomed 2011, 28, 269-274.
Search in Google Scholar
11. Mayser P., Laabs S., Heuer K.U., Gründer K.: Detection of extracellular phospholipase activity in Candida albicans and Rhodotorula rubra. Mycopathologia 1996, 135, 149-155.10.1007/BF006323369066156
Search in Google Scholar
12. Mirhendi H., Makimura K., Khoramizadeh M., Yamaguchi H.: A one-enzyme PCR-RFLP assay for identification of six medically important Candida species. Jpn J M Mycol 2006, 47, 225-229.10.3314/jjmm.47.22516940958
Search in Google Scholar
13. Panzio M.M., Reviákina V., Dolande M., Selgrad S.: Candida spp. in vitro study susceptibility profile to four antifungal agents. Resistance surveillance study in Venezuelan strains. Med Mycol 2009, 47, 137-143.10.1080/1369378080214433918651308
Search in Google Scholar
14. Porte L., Leon P., Garate C., Guzman A.M., Lebarca J., Garcia P.: Susceptibility to azoles and amphotericin B of isolates of Candida spp. Experience of a university health network, between 2004 and 2010. Revista Chilena de Infectologia 2012, 29, 149-155.10.4067/S0716-1018201200020000522689028
Search in Google Scholar
15. Sahiner F., Ergünay K., Ozyurt M., Ardiç N., Hoşbul T., Haznedaroglu T.: Phenotypic and genotypic identification of Candida strains isolated as nosocomial pathogens. Mikrobiyol Bul 2011, 45, 478-488.
Search in Google Scholar
16. Schmalreck A.F., Willinger B., Haase G., Blum G., Lass-Flörl C., Fegeler W., Becker K.: Species and susceptibility distribution of 1062 clinical isolates to azoles, echinocandins, flucytosine and amphotericin B from a multi-centre study. Mycoses 2012, 55, 24-37.10.1111/j.1439-0507.2011.02165.x22233267
Search in Google Scholar
17. Seker E.: Identification of Candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in Western Turkey. Mycopathologia 2010, 169, 303-308.10.1007/s11046-009-9255-z19921462
Search in Google Scholar
18. Shokohi T., Hashemi Soteh M.B., Sultanat Pouri Z., Hedayati M.T., Mayahi S.: Identification of Candida species using PCR-RFLP in cancer patients in Iran. Indian J Med Microbiol 2010, 28, 147-151.10.4103/0255-0857.6249320404462
Search in Google Scholar
19. Tortorano A.M., Caspani L., Rigoni A.L., Biraghi E., Sicignano A., Viviani M.A.: Candidosis in an intensive care unit: a 20-year survey. J Hosp Infect 2004, 57, 8-13.10.1016/j.jhin.2004.01.01715142710
Search in Google Scholar
20. Williams D.W.: Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA. J Clin Microbiol 1995, 33, 2476-2479.10.1128/jcm.33.9.2476-2479.19952284467494052
Search in Google Scholar
21. Xu J., Millar B.C., Moore J.E., McClurg R., Walker M.J., Evans J., Hedderwick S., McMullan R.: Comparison of API20C with molecular identification of Candida spp. isolated from bloodstream infections. J Clin Pathol 2002, 55, 774-777.10.1136/jcp.55.10.774176978712354806
Search in Google Scholar
22. Yang Y.L., Hsieh L.Y., Wang A.H., Lo H.J., TSARY Hospitals: Characterization of Candida species from different populations in Taiwan. Mycopathologia 2011, 172, 131-139. 10.1007/s11046-011-9408-821369746
Search in Google Scholar

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Abstract

Paradigm

My account