Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

Mogielnicka-Brzozowska, Marzena; Dziekońska, Anna; Strzeżek, Rafał; Załęcki, Michał; Majewska, Anna; Tołścik, Karolina; Kordan, Władysław

doi:10.2478/bvip-2014-0025

Abstract

Sperm surface binding sites for non-zinc-binding proteins (nZnBPs) and zinc-binding proteins (ZnBPs) were studied by the fluorescence technique with biotin-labelled proteins. The nZnBPs binding pattern was unspecific, no characteristic sites on plasmalemma were found. ZnBPs were attached mainly to the acrosomal region of sperm head and to the sperm flagellum. ZnBPs added to the incubation mixture of the canine spermatozoa allowed the preservation of higher values for total motility, progressive motility, curvilinear line velocity, straight line velocity, and beat cross frequency (P < 0.05), both at time 0 and after 1 h incubation at 5ºC. The addition of nZnBPs to the incubation mixture caused only weak positive effects when compared with control sample (PBS). A higher percentage of canine-ejaculated spermatozoa with intact membranes were observed when ejaculate was incubated with ZnBPs in comparison to control sample stored with PBS (P < 0.05) or nZnBPs (P < 0.05). Spermatozoa diluted with ZnBPs and nZnBPs exhibited a higher percentage of cells with active mitochondria when compared with control, both at time 0 and after 1 h; however, no statistical differences were observed. Our results emphasise the role of seminal plasma protein in securing the correct quantity and availability of zinc ions as a component regulating the motility of canine spermatozoa. The protective effect of ZnBPs against the cooling effect may be due to their ability of preventing sperm membrane damage.

References

1. Abdul-Rasheed O.F.: The relationship between seminal plasma zinc levels and high molecular weight zinc binding protein and sperm motility in Iraqi infertile men. Saudi Med J 2009, 30, 485-489.
Search in Google Scholar
2. Alvarez J.G., Storey B.T.: Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 1995, 42, 334-346.10.1002/mrd.1080420311
Search in Google Scholar
3. Barrios B., Fernandez-Juan M., Muino-Blanco T., Cebrian- Perez J.A.: Immunocytochemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock. J Androl 2005, 26, 539-549.10.2164/jandrol.04172
Search in Google Scholar
4. Bjorndhal L., Kvist U.: Influence of seminal vesicular fluid on the zinc content of human sperm chromatin. Int J Androl 1990, 13, 232-237.10.1111/j.1365-2605.1990.tb00981.x
Search in Google Scholar
5. Boursnell J.C., Noble E.A.: The absorption by boar spermatozoa of zinc bound to low molecular weight ligands. J Reprod Fertil 1975, 45, 421-426.10.1530/jrf.0.0450421
Search in Google Scholar
6. Caballero I., Vazquez J.M., Garcia E.M., Parrilla I., Roca J., Calvete J.J., Sanz L., Martinez E.A.: Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa. Theriogenology 2008, 70, 1352-1355.10.1016/j.theriogenology.2008.07.013
Search in Google Scholar
7. Caballero I., Vazquez J.M., Garcia E.M., Roca J., Martinez E.A., Calvete J.J., Sanz L., Ekwall H., Rodriguez-Martinez H.: Immunolocalization and possible functional role of PSP-I/PSP- II heterodimer in highly extended boar spermatozoa. J Androl 2006, 27, 766-773.10.2164/jandrol.106.000539
Search in Google Scholar
8. Calvete J.J., Raida M., Gentzel M., Urbanke C., Sanz L., Topfer -Petersen E.: Isolation and characterization of heparin and phosphorylcholine-binding proteins of boar and stallion seminal plasma. Primary structure of porcine pB1. FEBS Lett 1997, 407, 201-206.10.1016/S0014-5793(97)00344-X
Search in Google Scholar
9. Calvete J.J., Sanz L., Topfer-Petersen E.: Spermadhesins: structure-functions relationships. Assisted Reprod Technol/ Androl 1994, 6, 316-330.
Search in Google Scholar
10. Campanero-Rhodes M.A., Menendez M., Sainz L., Calvete J.J., Solis D.: Analysis of the stability of the spermadhesin PSP- I⁄PSP-II heterodimer. Effects of Zn2+ and acidic pH. FEBS J 2005, 272, 5663-5670.10.1111/j.1742-4658.2005.04974.x16262703
Search in Google Scholar
11. Dziekonska A., Fraser L., Strzezek J.: Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen. J Anim Feed Sci 2009, 18, 638-649.10.22358/jafs/66438/2009
Search in Google Scholar
12. Garner D.L., Johnson L.A.: Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 1995, 53, 276-284.10.1095/biolreprod53.2.2767492679
Search in Google Scholar
13. Henkel R., Bittner J., Weber M.D.R., Huther F., Miska W.: Relevance of zinc in human sperm flagella and its relation to motility. Fertil Steril 1999, 71, 1138-1143.10.1016/S0015-0282(99)00141-7
Search in Google Scholar
14. Holody D., Strzezek J.: Heparin- and Zn2+-binding proteins from boar seminal plasma. Acta Biochim Pol 1999, 46, 935-939.10.18388/abp.1999_4116
Search in Google Scholar
15. Lowry I.O., Rosebrough N.J., Farr A.L., Randall R.J.: Protein measurement with Folin phenol reagent. J Biol Chem 1951, 193, 265-275.10.1016/S0021-9258(19)52451-6
Search in Google Scholar
16. Manaskova P., Jonakova V.: Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa. J Reprod Immunol 2008, 78, 40-48.10.1016/j.jri.2007.10.00118031827
Search in Google Scholar
17. Mann T., Lutwak-Mann C.: Biochemistry of seminal plasma and male accessory fluids; application to andrological problems. In: Male reproductive function semen themes trends in physiology biochemistry investigative andrology. Edited by Mann T., Lutwak-Mann C., Springer-Verlag, Berlin, Heidelberg, New York, 1981, pp. 269-336.
Search in Google Scholar
18. Mogielnicka-Brzozowska M., Fraser L., Czarzasta J., Kordan W.: Isolation and characterization of zinc-binding proteins from canine seminal plasma. Pol J Vet Sci 2012, 15, 493-498.10.2478/v10181-012-0076-523214370
Search in Google Scholar
19. Mogielnicka-Brzozowska M., Wysocki P., Strzezek J., Kordan W.: Zinc-binding proteins from boar seminal plasma - isolation, biochemical characteristics and influence on spermatozoa stored at 4°C. Acta Biochim Pol 2011, 58, 171-177.10.18388/abp.2011_2261
Search in Google Scholar
20. Muino-Blanco T., Perez-Pe R., Cebrian-Perez J.A.: Seminal plasma proteins and sperm resistance to stress. Reprod Dom Anim 2008, 43, 18-31.10.1111/j.1439-0531.2008.01228.x18803753
Search in Google Scholar
21. Nothling J.O., Gerber D., Colenbrander B., Dijkstra M., Bakker T., De Cramer K.: The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed. Theriogenology 2007, 67, 264-275.10.1016/j.theriogenology.2006.07.01816962165
Search in Google Scholar
22. Nothling J.O., Shuttleworth R., de Haas K., Thompson P.N.: Homologous prostatic fluid added to frozen-thawed dog spermatozoa prior to intravaginal insemination of bitches resulted in better fertility than albumin-free TALP. Theriogenology 2005, 64, 975-991.10.1016/j.theriogenology.2005.01.00216054500
Search in Google Scholar
23. Riffo M., Leiva S., Astudillo J.: Effect of zinc on human sperm motility and the acrosome reactions. Int J Androl 1992, 15, 229-237.10.1111/j.1365-2605.1992.tb01343.x1399086
Search in Google Scholar
24. Robert M., Gagnon C.: Semenogelin I: a coagulum forming, multifunctional seminal vesicle protein. Cell Mol Life Sci 1999, 55, 944-960.10.1007/s000180050346
Search in Google Scholar
25. Rota A., Milani C., Romagnoli S.: Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status. Theriogenology 2007, 67, 520-525.10.1016/j.theriogenology.2006.09.001
Search in Google Scholar
26. Siciliano L., De Stefano C., Petroni M.F., Vivacqua A., Rago V., Carpino A.: Prostatic origin of a zinc binding high molecular weight protein complex in human seminal plasma. Mol Hum Reprod 2000, 6, 215-218.10.1093/molehr/6.3.215
Search in Google Scholar
27. Silvestroni L., Menditto A., Modesti A., Scarpa S.: Zinc uptake in human seminal spermatozoa: characterization and effects on cell membranes. Arch Androl 1989, 23, 97-103.10.3109/01485018908986830
Search in Google Scholar
28. Sirivaidyapong S., Ursem P., Bevers M.M., Colenbrander B.: Effect of prostatic fluid on motility, viability and acrosome integrity of chilled and frozen-thawed dog spermatozoa. J Reprod Fertil 2001, 57 Suppl, 383-386.
Search in Google Scholar
29. Strzezek J., Hopfer E., Zaborniak A.: Zinc ion dependent protein in boar semen. II. Effects on sperm motility and antibacterial properties. Anim Reprod Sci 1987, 13, 133-142.10.1016/0378-4320(87)90125-4
Search in Google Scholar
30. Strzezek R., Fraser L.: Characteristics of spermatozoa of whole ejaculate and sperm-rich fraction of dog semen following exposure to media varying in osmolality. Reprod Biol 2009, 9, 113-126.10.1016/S1642-431X(12)60021-7
Search in Google Scholar
31. Topfer-Petersen E., Romero A., Valera P.F., Ekhlasi-Hundrieser M., Dostalova Z., Sanz L., Calvete J.J.: Spermadhesins: a new protein family. Facts, hypotheses and perspectives. Andrologia 1998, 30, 217-224.10.1111/j.1439-0272.1998.tb01163.x9739418
Search in Google Scholar
32. Watson P.F.: Use of Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 1975, 97, 12-15.10.1136/vr.97.1.1249974
Search in Google Scholar

Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

Abstract

Paradigm

My account