Simultaneous determination of multi – component mycotoxin in feeds by liquid chromatography – tandem mass spectrometry

Katarzyna Pietruszka; Bartosz Sell; Olga Burek; Henryka Wiśniewska-Dmytrow

doi:10.2478/bvip-2013-0097

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Simultaneous determination of multi – component mycotoxin in feeds by liquid chromatography – tandem mass spectrometry

Journal of Veterinary Research

Volume 57 (2013): Issue 4 (December 2013)

By: Katarzyna Pietruszka, Bartosz Sell, Olga Burek and Henryka Wiśniewska-Dmytrow

Open Access

|Dec 2013

Abstract

A multiresidue method for determination and quantification of Fusarium mycotoxins: deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and metabolite of Aspergillus and Penicillium species - ochratoxin A in feeds was described. The method was based on the simultaneous extraction of selected mycotoxins from matrix, followed by liquid chromatography coupled with tandem mass spectrometry using a hybrid triple quadrupole - linear ion trap mass spectrometer with the multiple reaction monitoring in both positive- and negative-ion modes. The method was validated in accordance with the Commision Decision 2002/657/EC requirements. The mean recoveries of mycotoxins from spiked feed samples ranged from 74.6% to 113.9%, whereas limit of detection and quantification ranged from 0.72 to 12.4 μg/kg and 1.86 to 31.7 μg/kg, respectively.

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Articles in this issue

DOI: https://doi.org/10.2478/bvip-2013-0097 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 567 - 572

Published on: Dec 31, 2013

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

feeds,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2013 Katarzyna Pietruszka, Bartosz Sell, Olga Burek, Henryka Wiśniewska-Dmytrow, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons License.

Volume 57 (2013): Issue 4 (December 2013)