Figure 1.
Single base extension primers data
| Variant | Nucleotide change | PCR fragment size (bp) | SNaPshot primer orientation | SNaPshot result (N/M) | SNaPshot fragment size-N (bp) | SNaPshot fragment size-M (bp) |
|---|---|---|---|---|---|---|
| rs112782763 (c.-229G>A) | G/A | 551 | Forward | G/A | 27.1 | 30.3 |
| rs28717001 (c.-210A>C) | A/C | Reverse | T/G | 21.9 | 28.1 | |
| rs28651243 (c.-184T>C) | T/C | Reverse | A/G | 34.1 | 32 | |
| rs113588187 (c.-135G>A) | G/A | Reverse | C/T | 36.1 | 37.1 | |
| rs1570360 (c.1154G>A) | G/A | 450 | Forward | G/A | 37.5 | 38.5 |
| rs3025039 (c.*237C>T) | C/T | 427 | Reverse | G/A | 41.5 | 42.5 |
The general results of the variants in the ANXA5 gene in the group of women with EPLs and Control group
| Haplotype | Women with EPLs (n=190) | % | p-value | Controls (n=190) | % |
|---|---|---|---|---|---|
| N/N | 135 | 71.05 | 0.0009 | 161 | 84.73 |
| N/M1 | 15 | 7.89 | 0.09 | 8 | 4.21 |
| M1/M1 | 0 | 0.00 | / | 1 | 0.52 |
| N/M2 | 36 | 18.95 | 0.0006 | 14 | 7.37 |
| M2/M2 | 4 | 2.11 | 0.18 | 1 | 0.52 |
| M1/M2 | 0 | 0.00 | / | 5 | 7.90 |
| Total | 190 | 100.00 | 190 | 100.00 |
Nucleotide sequences of PCR primers
| Primer ID | Sequence (5′-3′) | Length (bp) |
|---|---|---|
| ANXA5-F | CAGCTACCGGGACAGCTC | 18 |
| ANXA5-R | CTCCAAAACCCCGAGCCC | 18 |
| VEGFA-F_IGU | TTCCTAGCAAAGAGGGAACG | 20 |
| VEGFA-R_IGU | GCTGACCGGTCCACCTAAC | 19 |
| VEGFA-F1_3’utr | ACACCATCACCATCGACAGA | 20 |
| VEGFA-R1_3’utr | GTCAGGATCTGAGTGGGAACA | 21 |
| rs112782763_ SShot | CCCCCCGCGGCCGGCCTGCGGTTG | 24 |
| rs28717001_ SShot | CCCCCCCTGCCCGGCTTGGCCCG | 23 |
| rs28651243_ SShot | CCCCCCCCCCCCCCCCGGAAACGCCAGCGGCCCC | 34 |
| rs113588187_ SShot | CCCCCCCGCCGAGATGCAGACGCTGAAGGATC | 32 |
| rs1570360_Sshot | CCCCCCCCCCCCCCCCGCCCGAGCCGCGTGTGGA | 34 |
| rs3025039_Sshot | CCCCCCCCCCCCCCTGGCGAATCCAATTCCAAGAGGGACC | 40 |
The genotypic frequency of the examined VEGFA variants in the group of women with EPLs and Control groups_
| c.-1154G>A | Women with EPLs (n=190) | % | p-value | Controls (n=190) | % |
|---|---|---|---|---|---|
| GG | 92 | 48.42 | 0.47 | 99 | 52.11 |
| GA | 76 | 40.00 | 0.53 | 70 | 36.84 |
| AA | 22 | 11.58 | 0.87 | 21 | 11.05 |
| Total | 190 | 100.00 | 190 | 100.00 | |
| c.*237C>T | |||||
| CC | 137 | 72.10 | 0.64 | 141 | 74.21 |
| CT | 50 | 26.32 | 0.64 | 46 | 24.21 |
| TT | 3 | 1.58 | 1 | 3 | 1.58 |
| Total | 190 | 100.00 | 190 | 100.00 |