Figure 1.
Structure of immunoglobulin heavy chain variable region.
VH – variable region of heavy chain; VL – variable region of light chain; V – variable segment; D – diversity segment; J – junctional segment; CDR – complementarity-determining region; FR – framework region
Figure 2.
Locations of the primers for V-D-J gene rearrangements.
5′ IGHV Leader primers are located upstream of the IGHV coding sequence; 5′ IGHV FR1 (framework) primers are located within the rearranged IGHV gene; 3′ IGHJ primers are located at the end of the rearranged IGHJ gene; UTR – untranslated region
Figure 3.
Distribution of Bulgarian CLL patients by their IGHV mutational status.
57 patients – unmutated IGHV; 44 patients – mutated IGHV; 4 patients – Borderline IGHV
Table 1.
Clinico-biological features and genetic characteristics of mutated and unmutated cases
| Patients | Leukocytes count (4.5 to 10×109 /L)* | General condition (Fit) | Treatment | 17p deletion | Common IGHV genes | BCR stereotyped subsets (n) |
|---|---|---|---|---|---|---|
| 44 mutated | 15–180 | Good condition | Newly diagnosed; Before first treatment | Negative |
| CLL#77 (1) |
| 57 unmutated | 17–328 |
|
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