Table 1.
Quantitative scoring system of stratified squamous epithelium by immunohistochemistry for AR, ER-α and ER-β.
| Score | Grade |
|---|---|
| 0 | No involvement |
| + | Involvement in basal keratinocytes |
| ++ | Involvement limited to the lower 1/3 of the epidermis |
| +++ | Involvement limited to the lower 2/3 of the epidermis |
| ++++ | Involvement in all epidermis layers |
Figure 1.
AR uptake was observed in basal keratinocytes in control group. AR involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
Figure 2.
ER-α uptake was observed in basal keratinocytes in control group. ER-α involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
Figure 3.
ER-β uptake was observed in basal keratinocytes in control group. ER-β involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
Table 2.
Quantitative scoring results of stratified squamous epithelium for AR and ERs (n=20).
| AR | Estrogen Receptor-α | Estrogen Receptor-β | ||||
|---|---|---|---|---|---|---|
| Score | Control | Hypospadias | Control | Hypospadias | Control | Hypospadias |
| 0 | 1 | - | 2 | - | - | - |
| + | 18 | - | 17 | - | 18 | - |
| ++ | 1 | 5 | 1 | 4 | 2 | 3 |
| +++ | - | 15 | - | 16 | - | 17 |
| ++++ | - | - | - | - | - | - |
Figure 4.
In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
Figure 5.
In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
Figure 6.
More than 50% involvement of keratinocytes (Epidermis Pattern 3) and clustered positive cells more than 50% of all visible dermal cells (Dermis Pattern C) for FGF-10 in the control group. In the hypospadias group, less than 50% of keratinocytes were involved (Epidermis Pattern 2) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
Table 3.
Epidermis and dermis involvement of FGFR-2, FGF-8, FGF-10(n=20).
| FGFR-2 | FGF-8 | FGF-10 | |||||
|---|---|---|---|---|---|---|---|
| Pattern | Control | Hypospadias | Control | Hypospadias | Control | Hypospadias | |
| Epidermis | 1 | - | - | - | 16 | - | 17 |
| 2 | 4 | - | 5 | 4 | 2 | 3 | |
| 3 | 16 | 5 | 15 | - | 18 | - | |
| Dermis | A | - | 1 | - | 3 | - | 2 |
| B | 3 | 19 | 14 | 17 | 3 | 18 | |
| C | 17 | - | 6 | - | 17 | - | |
Table 4.
Mean ± SD values of the markers in the groups for IHC evaluation.
| Control | Hypospadias | |
|---|---|---|
| Androgen Receptor | 2±0,32 | 3,75±0,44* |
| Estrogen Receptor-α | 1,95±0,39 | 3,80±0,41* |
| Estrogen Receptor-β | 2,10±0,30 | 3,85±0,36* |
| FGFR-2 Epidermis | 3,80±0,41 | 2±0,32* |
| FGFR-2 Dermis | 2,85±0,36 | 1,95±0,22* |
| FGF-8 Epidermis | 3,75±0,44 | 2,15±0,48* |
| FGF-8 Dermis | 2,30±0,47 | 1,85±0,36** |
| FGF-10 Epidermis | 3,90±0,30 | 2,15±0,36* |
| FGF-10 Dermis | 2,85±0,36 | 1,90±0,30* |