Have a personal or library account? Click to login
Comparison of FGF-8, FGF-10, FGF-Receptor 2, Androgen Receptor, Estrogen Receptor-A and SS in Healthy and Hypospadiac Children Cover

Comparison of FGF-8, FGF-10, FGF-Receptor 2, Androgen Receptor, Estrogen Receptor-A and SS in Healthy and Hypospadiac Children

Balkan Journal of Medical Genetics
Volume 27 (2024): Issue 1 (June 2024)
By: N Emaratpardaz,  Z Turkyilmaz,  R Karabulut,  D Dayanir,  C Kaya,  AAE Sert,  G Arkan,  FA Ucaner,  A Kapisiz,  S Eryilmaz,  A Atan and  K Sonmez  
Open Access
|Sep 2024

Figures & Tables

Figure 1.

AR uptake was observed in basal keratinocytes in control group. AR involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
AR uptake was observed in basal keratinocytes in control group. AR involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 2.

ER-α uptake was observed in basal keratinocytes in control group. ER-α involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
ER-α uptake was observed in basal keratinocytes in control group. ER-α involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 3.

ER-β uptake was observed in basal keratinocytes in control group. ER-β involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
ER-β uptake was observed in basal keratinocytes in control group. ER-β involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 4.

In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Figure 5.

In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Figure 6.

More than 50% involvement of keratinocytes (Epidermis Pattern 3) and clustered positive cells more than 50% of all visible dermal cells (Dermis Pattern C) for FGF-10 in the control group. In the hypospadias group, less than 50% of keratinocytes were involved (Epidermis Pattern 2) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
More than 50% involvement of keratinocytes (Epidermis Pattern 3) and clustered positive cells more than 50% of all visible dermal cells (Dermis Pattern C) for FGF-10 in the control group. In the hypospadias group, less than 50% of keratinocytes were involved (Epidermis Pattern 2) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Quantitative scoring system of stratified squamous epithelium by immunohistochemistry for AR, ER-α and ER-β_

ScoreGrade
0No involvement
+Involvement in basal keratinocytes
++Involvement limited to the lower 1/3 of the epidermis
+++Involvement limited to the lower 2/3 of the epidermis
++++Involvement in all epidermis layers

Quantitative scoring results of stratified squamous epithelium for AR and ERs (n=20)_

AREstrogen Receptor-αEstrogen Receptor-β
ScoreControlHypospadiasControlHypospadiasControlHypospadias
01-2---
+18-17-18-
++151423
+++-15-16-17
++++------

Mean ± SD values of the markers in the groups for IHC evaluation_

ControlHypospadias
Androgen Receptor2±0,323,75±0,44*
Estrogen Receptor-α1,95±0,393,80±0,41*
Estrogen Receptor-β2,10±0,303,85±0,36*
FGFR-2 Epidermis3,80±0,412±0,32*
FGFR-2 Dermis2,85±0,361,95±0,22*
FGF-8 Epidermis3,75±0,442,15±0,48*
FGF-8 Dermis2,30±0,471,85±0,36**
FGF-10 Epidermis3,90±0,302,15±0,36*
FGF-10 Dermis2,85±0,361,90±0,30*

Epidermis and dermis involvement of FGFR-2, FGF-8, FGF-10(n=20)_

FGFR-2FGF-8FGF-10
PatternControlHypospadiasControlHypospadiasControlHypospadias
Epidermis1---16-17
24-5423
316515-18-
DermisA-1-3-2
B3191417318
C17-6-17-
DOI: https://doi.org/10.2478/bjmg-2024-0002 | Journal eISSN: 2199-5761
Journal RSS Feed
Language: English
Page range: 21 - 29
Published on: Sep 6, 2024
Published by: Macedonian Academy of Sciences and Arts
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
hypospadias,
FGF receptors,
androgen receptor,
estrogen receptors,
etiology
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2024 N Emaratpardaz, Z Turkyilmaz, R Karabulut, D Dayanir, C Kaya, AAE Sert, G Arkan, FA Ucaner, A Kapisiz, S Eryilmaz, A Atan, K Sonmez, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Previous articleVolume 27 (2024): Issue 1 (June 2024)Next article