Figure 1.
Figure 2.
Figure 3.
Figure 4.
Mean Values of Biochemical Parameters for KLOTHO Gene
| Parameter | Methylated KLOTHO | Unmethylated KLOTHO | ||
|---|---|---|---|---|
| Hypertensive subjects | Control Subjects | Hypertensive subjects | Control subjects | |
| Fasting glucose (mg/dL) | 129.96 ± 83.08 (54) | 97.97 ± 12.43 (38) | 117.20 ± 34.03 (15) | 98.18 ± 5.23 (11) |
| Triglyceride (mg/dL) | 166.82 ±91.28 (38) | 122.22 ± 31.10 (37) | 183.93 ±104.06 (14) | 132.00±28.43 (11) |
| Total cholesterol (mg/dL) | 195.97 ± 61.63 (36) | 170.59 ±43.12 (37) | 197.07 ± 49.94 (14) | 191.09 ± 42.03 (11) |
| HDL-C (mg/dL) | 45.84 ± 14.31 (37) | 52.19 ± 12.72 (37) | 46.50 ± 14.34 (14) | 49.64 ± 6.83 (11) |
| LDL-C (mg/dL) | 131.92 ± 65.55 (37) | 129.62 ± 28.28 (37) | 125.71 ± 60.77 (14) | 130.27 ± 33.02 (11) |
| Sodium (Na) (mmol/L) | 137.98 ± 4.29 (53) | 138.26 ± 7.50 (38) | 136.60 ± 12.98 (15) | 143.27 ± 8.60 (11) |
| Potassium (K) (mmol/L) | 4.45 ± 0.60 (53) | 4.88 ± 1.11 (38) | 4.71± 0.75 (15) | 5.55 ± 1.20 (11) |
| Creatinine (Cr) (mg/dL) | 1.11± 0.59 (54) | 0.66 ± 0.14 (38) | 1.06 ± 0.95 (15) | 0.65 ± 0.11 (11) |
| Urea (mg/dL) | 28.66 ± 23.10 (53) | 15.18 ± 3.40 (38) | 21.14 ± 17.82 (14) | 20.91 ± 13.40 (11) |
The Interaction Between ARNTL and KLOTHO Methylation Status
| Subjects (p + c) | |||
|---|---|---|---|
| Methylation Status of ARNTL+KLOTHO Genes | Count | % | p value |
| ARNTL Methylated+KLOTHO Methylated | 53 | 46.1% | p>0.05 |
| ARNTL Methylated+KLOTHO Unmethylated | 14 | 12.1% | |
| ARNTL Unmethylated+KLOTHO Methylated | 38 | 33.0% | |
| ARNTL Unmethylated+KLOTHO Unmethylated | 10 | 8.8% | |
| Total | 115 | 100% | |
Mean Values of Biochemical Parameters for ARNTL Gene
| Parameter | Methylated ARNTL | Unmethylated ARNTL | ||
|---|---|---|---|---|
| Hypertensive subjects | Control Subjects | Hypertensive subjects | Control subjects | |
| Fasting glucose (mg/dL) | 120.00 ± 70.48 (42) | 96.67 ± 15.54 (21) | 142.65 ± 87.50 (23) | 98.14 ± 6.02 (21) |
| Triglyceride (mg/dL) | 177.58 ±98.15 (33) | 132.50 ± 31.30 (20) | 172.06±91.38 (16) | 120.57±28.13 (21) |
| Total cholesterol (mg/dL) | 197.58 ± 59.89 (33) | 179.60 ±59.04 (20) | 195.07 ± 52.06 (14) | 168.14 ± 28.81 (21) |
| HDL-C (mg/dL) | 47.55 ± 15.91 (33) | 48.55 ± 8.75 (20) | 41.87 ± 10.33 (15) | 53.48 ± 12.15 (21) |
| LDL-C (mg/dL) | 128.30 ± 60.90 (33) | 134.65 ± 35.32 (20) | 136.93 ± 71.65 (15) | 128.24 ± 26.19 (21) |
| Sodium (Na) (mmol/L) | 137.38 ± 8.36 (42) | 138.67 ± 6.63 (21) | 138.00 ±4.57 (22) | 141.00 ± 9.11 (21) |
| Potassium (K) (mmol/L) | 4.45 ± 0.70 (42) | 5.02 ± 1.05 (21) | 4.59± 0.57 (22) | 5.11 ± 1.30 (21) |
| Creatinine (Cr) (mg/dL) | 1.00± 0.51 (42) | 0.64 ± 0.15 (21) | 1.07 ± 0.52 (23) | 0.68 ± 0.12 (21) |
| Urea (mg/dL) | 26.78 ± 23.29 (40) | 17.95 ± 10.51 (21) | 23.22 ± 13.49 (23) | 15.76 ± 2.95 (21) |
KLOTHO and ARNTL gene methylation in hypertensive and control subjects
| Subjects | KLOTHO gene | ARNTL gene | ||||||
|---|---|---|---|---|---|---|---|---|
| Methylation | Unmethylation | Methylation | Unmethylation | |||||
| Count | %A | Count | %B | Count | %A | Count | %B | |
| Hypertension | 62 | 62.0% | 16 | 59.3% | 46 | 68.7% | 27 | 56.2% |
| Control | 38 | 38.0% | 11 | 40.7% | 21 | 31.3% | 21 | 43.8% |
| Total | 100 | 100% | 27 | 100% | 67 | 100% | 48 | 100% |
| p Value | p>0.05 | p>0.05 | ||||||