Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
The differentially expressed lncRNAs (Fold change> 2)
| LncRNA | Gene | Trend | CHR | Strand |
|---|---|---|---|---|
| ENST00000417781.5 | CSE1L-AS1 | Up | chr20 | − |
| ENST00000418924.6 | RIN3 | Up | chr14 | + |
| ENST00000440408.5 | TTTY15 | Up | chrY | + |
| ENST00000467315.5 | PFKL | Up | chr21 | + |
| ENST00000470527.1 | CACHD1 | Up | chr1 | + |
| ENST00000481985.5 | RPL3 | Up | chr22 | − |
| ENST00000488606.5 | MRPS15 | Up | chr1 | − |
| ENST00000497617.1 | TSFM | Up | chr12 | + |
| ENST00000504497.1 | DMXL1 | Up | chr5 | + |
| ENST00000530931.1 | CD82 | Up | chr11 | + |
| ENST00000460278.5 | ANKRD28 | Down | chr3 | − |
| ENST00000544168.5 | AKT1 | Down | chr14 | − |
| ENST00000611549.4 | RAP1GAP | Down | chr1 | − |
| ENST00000491117.5 | GNA12 | Down | chr7 | − |
| ENST00000610076.1 | KCNT2 | Down | chr1 | − |
| ENST00000601034.2 | INTS6-AS1 | Down | chr13 | + |
| ENST00000570265.5 | C15orf41 | Down | chr15 | + |
| ENST00000592944.1 | ITGA2B | Down | chr17 | − |
| ENST00000494731.5 | ZDHHC20 | Down | chr13 | − |
| ENST00000628791.1 | AC093495.1 | Down | chr3 | + |
The primers sequence for qPCR
| Gene name | Primer (5′-3′) | |
|---|---|---|
| ENST00000470527.1 | Forward | TGGAATTCGATGGGAACTTT |
| Reverse | GTCTCGTCCTGGATTGAAGG | |
| ENST00000504497.1 | Forward | TCGATTCTCCTGTCAGTGAAC |
| Reverse | AATGTTTCCAGAGCACCACT | |
| ENST00000417781.5 | Forward | GTTGATCGATCCAAGGTCGT |
| Reverse | GCCTGGAATCCCAGCATTT | |
| ENST00000440408.5 | Forward | TGCTTGGACAACAGACATGA |
| Reverse | GAAGCAATGTAATCCCAGCA | |
| GAPDH | Forward | AACTTTGGCATTGTGGAAGG |
| Reverse | GGATGCAGGGATGATGTTCT | |
The differentially expressed mRNAs (Fold change> 2)
| mRNA | lncRNA | Trend | CHR | Strand |
|---|---|---|---|---|
| AC027796.3 | ENSG00000262304.2 | Up | chr17 | − |
| AQP7 | ENSG00000165269.12 | Up | chr9 | − |
| ARNT2 | ENSG00000172379.20 | Up | chr15 | + |
| DGCR6 | ENSG00000183628.12 | Up | chr22 | + |
| GRID2IP | ENSG00000215045.8 | Up | chr7 | − |
| MICU3 | ENSG00000155970.11 | Up | chr8 | + |
| MROH7-TTC4 | ENSG00000271723.5 | Up | chr1 | + |
| RHOXF1 | ENSG00000101883.4 | Up | chrX | − |
| ZNF683 | ENSG00000176083.17 | Up | chr1 | − |
| AC046185.1 | ENSG00000125695.12 | Down | chr17 | − |
| AC137834.1 | ENSG00000258830.1 | Down | chr12 | − |
| AL136295.1 | ENSG00000254692.1 | Down | chr14 | − |
| BLOC1S5-TXNDC5 | ENSG00000259040.5 | Down | chr6 | − |
| CTSV | ENSG00000136943.10 | Down | chr9 | − |
| CYP3A5 | ENSG00000106258.13 | Down | chr7 | − |
| GABRE | ENSG00000102287.18 | Down | chrX | − |
| GSTM5 | ENSG00000134201.10 | Down | chr1 | + |
| KCNT2 | ENSG00000162687.16 | Down | chr1 | − |
| MRAP2 | ENSG00000135324.5 | Down | chr6 | + |
| MYZAP | ENSG00000263155.5 | Down | chr15 | + |
| PKDCC | ENSG00000162878.12 | Down | chr2 | + |
| PPP1R14C | ENSG00000198729.4 | Down | chr6 | + |
| SH3D19 | ENSG00000109686.17 | Down | chr4 | − |
| SLC2A14 | ENSG00000173262.11 | Down | chr12 | − |
Clinical characteristics of the three groups
| Group | G1 (N=5) | G2 (N=5) | G3 (N=5) | P valve |
|---|---|---|---|---|
| Gestational age (week) | 32.54±2.35 | 32.14±3.47 | 31.11±1.15 | 0.66 |
| Birth weight (g) | 1654.00±540.81 | 1622.00±503.16 | 1473.00±274.94 | 0.81 |
| Apgar score at 5 min | 9.20±0.84 | 8.40±1.14 | 8.40±1.14 | 0.41 |
| Male (%) | 40.00 | 40.00 | 20.00 | 0.78 |
| Cesarean section (%) | 40.00 | 40.00 | 60.00 | 0.80 |
| Twins (%) | 20.00 | 0 | 20.00 | 0.62 |
| Gestational diabetes (%) | 60.00 | 40.00 | 40.00 | 0.80 |
| Without glucocorticoid usage before delivery (%) | 40.00 | 20.00 | 40.00 | 0.78 |