Figure 1.
Figure 2.
The detailed PCR primers of each gene
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| HIF-1α | 5′-TCCATGTGACCATGAGGAAA-3′ | 5′-CC AAGCAGGTCATAGGTGGT-3′ |
| KDM3A | 5′-GGCGGACTTTAGACGTTCCA-3′ | 5′-AGATGAGCCTTCCACTTGGC-3′ |
| E-cadherin | 5′-CGGGAATGCAGTTGAGGATC-3′ | 5′-AGGATGGTGTAAGCGATGGC-3′ |
| Claudin-1 | 5′-GATAGCAATCTTTGTGGCCACCGT-3′ | 5′-TTCGTACCTGGCATTGACTGGG-3′ |
| Slug | 5′-CATGCCTGTCATACCACAAC-3′ | 5′-GGTGTCAGATGGAGGAGGG-3′ |
| ZEB-1 | 5′- GCCAATAAGCAAACGATTCTG-3′ | 5′- TTTGGCTGGATCACTTTCAAG-3′ |
| ACTB | 5′-GCCGGGACCTGACTGACTAC-3′ | 5′-TTCTCCTTAATGTCACGCACGAT-3′ |
Comparing the control and CRC groups gene expression levels
| Control | CRC | ||
|---|---|---|---|
| Log2(2-ΔΔCT) | Median [%25-%75] | Median [%25-%75] | p |
| HIF-1α | -1.32 [-5.19- 4.59] | 4.25 [0.72 -7.4] | 0.0045 |
| KDM3A | 0.06 [-3.56-3.12] | 4.29 [-0.74-5.97] | 0.0049 |
| E-cadherin | -1.19 [-4.40-4.03] | 0.46 [-2.89-4.01] | 0.189 |
| Slug | 0.56 [-3.18-2.64] | 2.74 [0.75-3.51] | 0.008 |
| ZEB-1 | 0.65 [-2.75-2.18] | 2.49 [-1.09-3.58] | 0.029 |
| Log2(2-ΔΔCT) | Mean±SD | Mean±SD | p |
| Claudin-1 | 0.00058±3.45 | 0.74±4.03 | 0.42 |