Single nucleotide polymorphisms in IL-1A RS1800587, IL-1B RS1143634 and vitamin D receptor rs731236 in stage III grade B/C periodontitis

Özturk, Özener H.; Tacal, Aslan B.; Eken, B.F.; Agrali, Ö.B.; Yildrim, H.S.; Altunok, E.Ç.; Ulucan, K.; Kuru, L.

Single nucleotide polymorphisms in IL-1A RS1800587, IL-1B RS1143634 and vitamin D receptor rs731236 in stage III grade B/C periodontitis

Balkan Journal of Medical Genetics

Volume 25 (2022): Issue 1 (June 2022)

By:

Özener H. Özturk, Aslan B. Tacal, B.F. Eken, Ö.B. Agrali, H.S. Yildrim, E.Ç. Altunok, K. Ulucan and L. Kuru

Open Access

|Mar 2023

Figures & Tables

Genotypic and allelic frequency of IL-1A, IL-1B and VDR polymorphisms in Grade B and C periodontitis and healthy population

Gene SNPs (rs number)	Genotypes and Alleles	Grade B Periodontitis N=51	Grade C Periodontitis N=49	Healthy N= 100 N (%)	Grade B Periodontitis VS Healthy			Grade C Periodontitis VS Healthy			Grade B Periodontitis VS Grade C Periodontitis
		N (%)	N (%)		χ² (p value)	OR (95% CI)	Fisher exact (p value)	χ² (p value)	OR (95% CI)	Fisher exact (p value)	χ² (p value)	OR (95% CI)	Fisher exact (p value)
ILIA⁸⁸⁹ (rsl800587)	CC	26(51)	23 (46.9)	56 (56)	0.608	0.718(0.179-2.874) 0.539(0.129-2.175)	0.749	0.403	1.623 (0.518-5.082) 1.167(0.362-3.756)	0.543	0.277	2.261 (0.507-10.07) 2.200(0.485-9.983)	0.470
	CT	22(43.1)	20(40.8)	35 (35)	0.323		0.515	0.796		1.000	0.300		0.485
	TT	3(5.9)	6(12.2)	9(9)
	C allele	74(72.5)	66(67.3)	147(73.5)	0.806	1.07(0.625-1.831)	0.890	0.241	1.372(0.809-2.323)	0.273	0.422	1.281 (0.699-2.349)	0.444
	T allele	28 (27.5)	32(32,7)	53 (26.5)
IL1B⁺³⁹⁵⁴ (rsll43634)	CC	29(56.9)	31(63.3)	74(74)	0.304	0.490(0.123-1.953) 1.071 (0.249-4.603)	0.445	0.635	0.698(0.157-3.103) 1.190(0.246-5.794)	0.695	0.659	0.702(0.144-3.407) 0.900(0.173-4.669)	0.709
	CT	18(35.3)	15(30.6)	21(21)	0.926		1.000	0.828		1.000	0.900		1.000
	TT	4(7.8)	3(6.1)	5(5)
	C allele	76(74.5)	77(78.6)	169(84.5)	0.036	1.865 (1.037-3.355)	0.043	0.205	0.673(0.363-1.245)	0.255	0.498	0.797(0.413-1.537)	0.510
	T allele	26 (25.5)	21(21.4)	31(15.5)
VDR TaqI (rs731236)	CC	9(17.6)	10(20.4)	10(10)	0.024	3.392(1.142- 10.075)	0.038	0.136	3.769(1.295-10.975) 0.831 (0.388-1.780)	0.188	0.564	1.592(0,515-4,922) 3.207(1.286-7.997)	0.300
	CT	29(56.9)	16(32.7)	41(41)	0.012	2.666(1.229-5.785)	0.015	0.634		0.702	0.011		0.014
	TT	13(25.5)	23 (46.9)	49(49)
	C allele	47(46.1)	36(36.7)	61(30.5)	0.008	1.947(1.190-3.185)	0.011	0.281	1.323 (0.795-2.202)	0.295	0.180	1.472(0.836-2.592)	0.198
	T allele	55 (53.9)	62(63.3)	139 (69.5)

Genotypic frequency of IL-1A, IL-1B and VDR polymorphisms in periodontitis and healthy population

Gene SNPs (rs number)	Genotype	Periodontitis N= 100 N (%)	Healthy N=100 N (%)	χ2 P value	OR (95% CI)	Fisher exact P value
IL-1A^-889 (rs1800587)	CC	49 (49)	56 (56)	0.793	1.142(0.420-3.107)	0.804
	CT	42 (42)	35 (35)	0.727	0.833(2.327-2.327)	0.796
	TT	9 (9)	9 (9)

	HWE	1.000	0.309

IL-1B⁺³⁹⁵⁴ (rs1143634)	CC	60 (60)	74 (74)	0.371	1.726 (0.52-5.716)	1.000
	CT	33 (33)	21 (21)	0.858	0.890 0.249-3.176)	0.333
	TT	7 (7)	5 (5)

	HWE	0.867	0.005

*VDR TaqI (rs731236)*	CC	19 (19)	10 (10)	0.031	2.586(1,074-6.224)	0.034
	CT	45 (45)	41 (41)	0.192	1.494 (0.817-2.732)	0.222
	TT	36 (36)	49 (49)

	HWE	0.464	0.742

Periodontal clinical parameters in study population

Parameters	Periodontitis N=100 Mean±SD	Healthy N=100 Mean±SD	P value
Age Range (years)	39.61±8.74 (22-59)	29.50±4.98 (21-55)	0.000*
Gender (%) Male Female	43 (43) 57 (57)	38 (38) 62 (62)	0.565†
PD (mm)	2.88±0.79	1.79±0.33	0.000*
CAL (mm)	3.14±0.88	1.81±1.79	0.000*
PI	1.70±0.45	0.34±0.20	0.000*
GI	1.56±0.41	0.26±0.23	0.000*
BOP (%)	58.82±21.91	8.77±6.25	0.000*

Model wise genotypic distribution of IL-1A, IL-1B and VDR in periodontitis and healthy population

Genotypes and alleles	Periodontitis N (%)	Healthy N (%)	OR (95% CI)	χ2 P value
IL1A^-889 (rs1800587)
C	140 (70)	147 (73.5)	1.188 (0.768-1.838)	0.437
T ref	60 (30)	53 (26.5)
Codominant model
CC	49 (49)	56 (56)	1.142 (0.420-3.107)	0.793
CT	42 (42)	35 (35)	0.833 (2.327-2.327)	0.727
TT ref	9 (9)	9 (9)
Dominant model
CC ref	49	56
CT+TT	51	44	0.754 (0.432-1.316)	0.322
Recessive model
CC+CT ref	91	91
TT	9	9	1.000 (0.380-2.634)	1.000
Overdominant model
CC+TT ref	58	65
CT	42	35	0.744 (0.420-1.317)	0.309
IL1B⁺³⁹⁵⁴ (rs1143634)
C	153 (76.5)	169 (84.5)	1.167 (1.012-2.770)	0.045
T ref	47 (23.5)	31 (15.5)
Codominant model
CC	60 (60)	74 (74)	1.726 (0.52-5.716)	0.371
CT	33 (33)	21 (21)	0.890 (0.249-3.176)	0.858
TT ref	7 (7)	5 (5)
Dominant model
CC ref	60 (60)	74 (74)
CT+TT	40 (40)	26 (26)	0.527 (0.289-0.960)	0.035
Recessive model
CC+CT ref	93 (93)	95 (95)
TT	7 (7)	5 (5)	0.699 (0.214-2.282)	0.522
Overdominant model
CC+TT ref	67 (67)	79 (79)	0.5397 (0,2855-1.021)	0.058
CT	33 (33)	21 (21)
*VDR TaqI (rs731236)*
T	117 (58.5)	139 (69.5)	1.616 (1.07-2.44)	0.022
C ref	83 (41.5)	61 (30.5)
Codominant model
TT	36 (36)	49 (49)	2.586 (1.074-6.224)	0.034
CT	45 (45)	41 (41)	1.73 (0.721-4.152)	0.218
CC ref	19 (19)	10 (10)
Dominant model
TT ref	36 (36)	49 (49)
CT+CC	64 (64)	51 (51)	0.585 (0.332-1.031)	0.063
Recessive model
TT+CT ref	81 (81)	90 (90)
CC	19 (19)	10 (10)	0.474 (0.208-1.078)	0.075
Overdominant model
TT+CC ref	55 (55)	59 (59)
CT	45 (45)	41 (41)	0.845 (0.485-1.487)	0.568

Allelic frequency of IL-1A, IL-1B and VDR polymorphisms in periodontitis and healthy population

Gene SNPs (rs number)	Allele	Periodontitis N= 100 N (%)	Healthy N=100 N (%)	χ2 P value	OR (95% CI)	Fisher exact P value
IL1A^-889	C	140 (70)	147 (73.5)	0.437	1.188 (0.768-1.838)	0.505
(rs1800587)	T	60 (30)	53 (26.5)
IL1B⁺³⁹⁵⁴	C	153 (76.5)	169 (84.5)	0.045	1.167 (1.012-2.770)	0.058
(rs1143634)	T	47 (23.5)	31 (15.5)
*VDR TaqI*	C	83 (41.5)	61 (30.5)	0.022	1.61 (1.071-2.441)	0.029
(rs731236)	T	117 (58.5)	139 (69.5)

DOI: https://doi.org/10.2478/bjmg-2022-0005 | Journal eISSN: 2199-5761

Journal RSS Feed

Language: English

Page range: 51 - 60

Published on: Mar 1, 2023

Published by: Macedonian Academy of Sciences and Arts

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Related subjects:

Basic medical science,

Basic medical science, other

© 2023 Özener H. Özturk, Aslan B. Tacal, B.F. Eken, Ö.B. Agrali, H.S. Yildrim, E.Ç. Altunok, K. Ulucan, L. Kuru, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Previous article Volume 25 (2022): Issue 1 (June 2022)Next article