Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

Öztan, G; Aktan, M; Palanduz, S; İşsever, H; Öztürk, S; Nikerel, E; Uçur, A; Bağatir, G; Bayrak, A; Çefle, K

doi:10.2478/bjmg-2020-0007

Figures & Tables

Scatterplot comparing the normalized expression of genes between the CLL patients and healthy controls. Genes involved in CLL cell cycle and cell growth, proliferation and differentiation and apoptosis are shown. The central line indicates unchanged gene expression. Red dots represent genes with a fold regulation of >2.

Illustration of interactions between the 18 differentially-expressed genes in CLL patients (String v10, confidence view).

The mean difference values of the patient and control group according to average ᐃCt and fold change_

Gene	Average Ct		Average ᐃCt		2^-ᐃCt		Fold Change		p Value	Fold Regulation
	CLL	Control	CLL	Control	CLL	Control	CLL/ Control	95% CI		CLL/ Control
APAF1	26.57	25.28	7.72	7.54	0.004695	0.005368	0.87	0.78-0.96	0.072729	-1.14
*ATM*	27.85	27.91	9.01	10.17	0.001936	0.000869	2.23	1.67-2.79	<0.001	2.23
*ATR*	29.00	29.41	10.17	11.67	0.000871	0.000307	2.83	2.23-3.43	<0.001	2.83
BAX	25.61	25.87	6.78	8.13	0.009116	0.003578	2.55	2.15-2.95	<0.001	2.55
*CASP9*	27.35	27.58	8.52	9.84	0.002723	0.001091	2.50	2.06-2.94	<0.001	2.50
*CDK4*	26.92	27.96	8.09	10.22	0.003672	0.000839	4.38	3.20-5.56	<0.001	4.38
CDKN1A	27.59	26.82	8.75	9.08	0.002315	0.001852	1.25	0.97-1.53	0.066	1.25
*CDKN2A*	30.50	32.77	11.67	15.02	0.000307	0.000030	10.22	7.14-13.3	<0.001	10.22
*CHEK1*	31.37	31.58	12.54	13.83	0.000168	0.000068	2.45	1.98-2.92	<0.001	2.45
*CHEK2*	29.45	30.42	10.62	12.68	0.000638	0.000152	4.18	3.02-5.34	<0.001	4.18
E2F1	31.44	30.88	12.60	13.14	0.000161	0.000111	1.45	1.04-1.86	0.012741	1.45
*E2F3*	28.88	27.09	10.05	9.34	0.000944	0.001541	0.61	0.53-0.69	<0.001	-1.63
GADD45A	31.94	31.14	13.10	13.39	0.000114	0.000093	1.22	0.90-1.54	0.121	1.22
*MCL1*	23.34	21.85	4.51	4.11	0.043950	0.057965	0.76	0.66-0.86	<0.001	-1.32
*MDM2*	25.77	25.60	6.93	7.86	0.008184	0.004312	1.90	1.56-2.24	<0.001	1.90
*MDM4*	27.43	28.00	8.60	10.26	0.002577	0.000818	3.15	2.32-3.998	<0.001	3.15
*PCNA*	25.75	26.36	6.92	8.62	0.008266	0.002545	3.25	2.64-3.86	<0.001	3.25
PTEN	25.53	24.05	6.50	6.31	0.011036	0.012615	0.87	0.78-0.96	0.062542	-1.14
*RB1*	26.51	27.04	7.67	9.29	0.004894	0.001595	3.07	2.40-3.74	<0.001	3.07
*P53*	25.76	26.32	6.92	8.58	0.008229	0.002619	3.14	2.47-3.81	<0.001	3.14
PTX3	32.50	30.53	13.67	12.79	0.000077	0.000141	0.54	0.37-0.71	0.006057	-1.84
*BCL2*	25.94	27.94	7.11	10.20	0.007231	0.000850	8.5'	5.46-11.56	<0.001	8.51

Patient diagnostic data and FISH results_

n	Sex-Age	Prognosis	Rai Stage	Binet Stage	FISH Results				(WBC 10⁹/L)	Hb (g/dL)	Platelet (10⁹/L)
					del(13q)	del(17p)	+12	Normal Non-del(13q), del(17p), +12.
1	F-75	bad	1	B	[–]	[+]	[–]	[–]	12.6	12.7	166.0
2	M-71	good	0	A	[–]	[–]	[–]	[+]	23.0	12.0	203.0
3	M-57	good	0	A	[–]	[–]	[–]	[+]	24.8	15.1	262.0
4	M-56	good	3	A	[–]	[–]	[–]	[+]	71.4	13.2	49.0
5	F-59	bad	2	B	[–]	[+]	[–]	[–]	7.9	10.9	284.0
6	F-74	bad	2	B	[–]	[+]	[–]	[–]	108.6	10.3	190.0
7	F-63	bad	2	A	[–]	[+]	[–]	[–]	35.4	13.4	185.0
8	M-80	bad	2	A	[–]	[+]	[–]	[–]	3.6	12.9	104.0
9	F-62	bad	0	A	[–]	[–]	[+]	[–]	190.2	12.0	192.0
10	M-67	bad	2	A	[–]	[–]	[+]	[–]	142.6	11.6	81.0
11	F-65	bad	0	A	[–]	[–]	[+]	[–]	5.2	12.1	144.0
12	F-74	bad	2	A	[–]	[–]	[+]	[–]	179.7	11.9	321.0
13	F-86	bad	3	A	[–]	[–]	[+]	[–]	213.0	8.6	113.0
14	M-60	good	3	A	[–]	[–]	[–]	[+]	51.7	9.4	137.0
15	M-46	good	2	A	[–]	[–]	[–]	[+]	15.0	14.4	184.0
16	M-54	good	0	A	[–]	[–]	[–]	[+]	41.9	13.8	352.0
17	F-61	good	1	A	[–]	[–]	[–]	[+]	11.8	12.1	148.0
18	F-67	good	0	A	[–]	[–]	[–]	[+]	195.3	10.8	188.0
19	F-53	good	2	B	[–]	[–]	[–]	[+]	39.8	11.4	135.0
20	M-67	good	0	A	[+]	[–]	[–]	[–]	74.6	12.4	183.0
21	M-62	good	2	A	[+]	[–]	[–]	[–]	121.4	14.5	294.0
22	M-68	good	0	A	[+]	[–]	[–]	[–]	37.7	13.9	197.0
23	F-46	good	1	A	[+]	[–]	[–]	[–]	59.4	12.3	91.0
24	M-58	good	2	B	[+]	[–]	[–]	[–]	201.4	11.1	101.0
25	M-66	good	0	A	[+]	[–]	[–]	[–]	43.9	12.3	88.0
26	M-61	good	4	C	[+]	[–]	[–]	[–]	110.0	13.9	39.0
27	M-51	good	2	A	[+]	[–]	[–]	[–]	98.5	14.3	250.0
28	F-78	good	3	B	[+]	[–]	[–]	[–]	13.7	11.0	324.0
29	F-63	good	0	A	[+]	[–]	[–]	[–]	113.3	11.5	178.0
30	M-64	good	0	A	[+]	[–]	[–]	[–]	432.0	15.3	206.0

Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

Figures & Tables

Figure 1

Figure 2

The mean difference values of the patient and control group according to average ᐃCt and fold change_

Patient diagnostic data and FISH results_

Paradigm

My account