Comparison of expression levels of miRNA in the patient and control groups_
| miRNA | AVG Ct | 2[-AvgΔ(Ct)] | p Value | Ratio Patient/Control | ||
|---|---|---|---|---|---|---|
| Control | Patient | Control | Patient | |||
| miR-143 | 26.00 | 25.04 | 0.136139 | 0.203892 | 0.025721 | 1.4977 |
| miR-142-5p | 26.80 | 24.68 | 0.078435 | 0.26217 | 0.000003 | 3.3425 |
Distribution of disease characteristics_
| Patient Group (n=50) | n(%) | |
|---|---|---|
| HLA-B27 | negative positive | 23 (46.0) 27 (54.0) |
| ESR | median (min-max) mean+SD | 17 (2-59) 21.36±15.51 |
| Symptom duration (years) | median (min-max) mean+SD | 5 (1-25) 6.60±5.08 |
| BASDAI | median (min-max) mean±SD | 2.5 (0-8) 2.75±2.03 |
| BASF | median (min-max) mean+SD | 5(5-12) 5.36±1.19 |
| BASMI | median (min-max) mean+SD | 1 (0-9.8) 1.55±1.93 |
| miR-142-5p | median (min-max) mean±SD | 24.1 (21.4-30.5) 24.68±2.06 |
| miR-143 | median (min-max) mean+SD | 24.2 (21.6-35.8) 25.10±3.11 |
| Control Group (n=50) | n (%) | |
| miR-142-5p | median (min-max) mean±SD | 26.4 (22.45-34.7) 26.88±2.88 |
| miR-143 | median (min-max) mean+SD | 25.4(21.28-33.7) 26.02±2.71 |
Correlation between age and disease characteristics and miR-142-5p and miR-143 measurements_
| Patient Group (n=50) | miR-142-5p | miR-143 | ||
|---|---|---|---|---|
| r Pearson correlation coefficient. | p Value | r Spearman’s correlation coefficient. | p Value | |
| Age (years) | 0.047 | 0.746 | 0.126 | 0.384 |
| ESR | -0.070 | 0.641 | -0.081 | 0.589 |
| Symptom duration (years) | -0.184 | 0.200 | -0.172 | 0.233 |
| BASDAI | -0.268 | 0.060 | -0.266 | 0.062 |
| BASFI | -0.026 | 0.861 | -0.114 | 0.447 |
| BASMI | 0.062 | 0.671 | 0.229 | 0.110 |
Distribution of descriptive characteristics_
| Descriptive Characteristics (n=100) | ||
|---|---|---|
| Age (years) | median (min-max) | 37(18-60) |
| mean±SD | 37.24±9.42 | |
| Gender n (%) | females | 58 (58.0) |
| males | 42 (42.0) | |
miRNAs analyzed in the signaling pathway targeted miScript miRNA PCR array study_
| T-Cell Differentiation CD4-/CD8- T-cell associated: | let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p, miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p |
| CD4+/CD8+ T-cell associated: | let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p. |
| CD4+ Naive T-cell associated: | miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p |
| CD8+ Naive T-cell associated: | let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p |
| CD8+ Effector cell associated: | miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p |
| CD8+ Memory cell associated: | let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p |
| B-Cell Differentiation | let-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p, |
| Naive B-cell associated: | miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p |
| Germinal centre associated: | miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p, miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p |
| Memory cell associated: | miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p |
| Expressed in the Treg cells: | miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p, miR-335-5p, miR-365a-3p, miR-99a-5p |
| T-cell activation associated: | let-7e-5p, let-7g-5p, let-7i-5p, miR-143, miR-140-3p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-368, miR-574-3p, miR-98-5p |