Table 1
The main characteristics of the coronary artery disease patients and controls. (Values are presented as mean ± SD and percent.)
| Parameters | CAD Patients (n = 112) | Controls (n = 56) | p Valuea |
|---|---|---|---|
| Gender (F; M) | F: 22; M: 90 | F: 30; M: 26 | <0.001 |
| Age (years) | 62.63 ± 9.16 | 56.40 ± 9.40 | <0.001 |
| Hypertension | 76 (67.9%) | 34 (60.7%) | 0.384 |
| DMT2 | 52 (46.2%) | 20 (35.7%) | 0.244 |
| Current smoker | 66 (58.9%) | 29 (51.8%) | 0.406 |
| LDL-cholesterol (mg/dL) | 114.99 ± 43.28 | 104.67 ± 32.47 | 0.463 |
| HDL-cholesterol (mg/dL) | 44.61 ± 13.49 | 41.33 ± 12.14 | 0.228 |
| Total cholesterol (mg/dL) | 190.81 ± 52.96 | 176.42 ± 39.45 | 0.310 |
| Triglycerides (mg/dL) | 176.22 ± 106.12 | 149.26 ± 68.27 | 0.234 |
| Fasting glucose (mg/dL) | 122.21 ± 47.81 | 109.79 ± 24.89 | 0.215 |
| eGFR (mL/min./1.73m3) | 90.37 ± 19.83 | 84.83 ± 22.81 | 0.051 |
| sMGP (μg/L) | 0.82 ± 0.37 | 0.87 ± 0.42 | 0.426 |
[i] CAD: coronary artery disease; DMT2: Diabetes mellitus type II; eGFR: estimated glomerular filtration rate; sMGP: serum MGP.
a Adjusted for age and gender status.
Figure 1
The structure of the human MGP gene showing the location of the rs4236, rs12304 and rs1800802 polymorphisms.
Table 2
Genotype and allele distributions of matrix Gla protein gene rs1800802, rs4236, and rs12304 polymorphisms.
| Genotype/Allele | CAD Patients (n = 112) | Controls (n =56) | p Value |
|---|---|---|---|
| rs1800802 | |||
| Genotype: TT | 42 (37.5%) | 22 (39.3%) | |
| TC | 55 (49.1%) | 29 (51.8%) | 0.701 |
| CC | 15 (13.4%) | 5 (8.9%) | |
| Allele: T | 139 (62.0%) | 73 (65.2%) | |
| C | 85 (38.0%) | 39 (34.8%) | 0.822 |
| rs4236 | |||
| Genotype: Thr/Thr | 37 (33.0%) | 12 (21.4%) | 0.267 |
| Thr/Ala | 57 (50.9%) | 35 (62.5%) | |
| Ala/Ala | 18 (16.1%) | 9 (16.1%) | |
| Allele: Thr | 131 (58.5%) | 59 (52.7%) | |
| Ala | 93 (41.5%) | 53 (47.3%) | 0.119 |
| rs12304 | |||
| Genotype: Glu/Glu | 89 (79.5%) | 44 (80.0%) | 0.936 |
| Glu/X | 23 (20.5%) | 11 (20.0%) | |
| X/X | 0 (0.0%) | 0 (0.0%) | |
| Allele: Glu | 201 (89.7%) | 99 (90.0%) | 0.936 |
| X | 23 (10.3%) | 11 (10.0%) |
[i] CAD: coronary artery disease.
Figure 2
Histograms of the serum MGP concentrations (μg/L) relative to MGP genotypes. The statistical significance between MGP concentrations were indicated by letters ‘a’ and ‘b.’
Table 3
Odds ratios and their 95% confidence intervals for alleles of matrix Gla protein genotypes for coronary artery disease.
| Genotypes | CAD Patients (n = 112) | Single-Vessel Disease (n = 62) | Two-Vessel Disease (n = 30) | Three-Vessel Disease (n = 20) | ||||
|---|---|---|---|---|---|---|---|---|
| OR (95% CI) | p Valuea | OR (95% CI) | p Valuea | OR (95% CI) | p Valuea | OR (95% CI) | p Valuea | |
| rs1800802: TT vs. | ||||||||
| TT+CC | 0.927 (0.480-1.792) | 0.822 | 0.736 (0.346-1.567) | 0.426 | 1.182 (0.481-2.905) | 0.716 | 1.264 (0.451-3.457) | 0.655 |
| rs4236: Thr/Thr vs. | ||||||||
| Thr/Ala+Ala/Ala | 1.809 (0.854-3.829) | 0.121 | 1.878 (0.821-4.294) | 0.135 | 1.833 (0.680-4.943) | 0.231 | 1.571 (0.498-4.962) | 0.441 |
| rs12304: Glu/Glu vs. | ||||||||
| Glu/X+X/X | 0.967 (0.433-2.162) | 0.936 | 0.857 (0.352-2.086) | 0.734 | 1.625 (0.469-5.631) | 0.444 | 0.750 (0.224-2.512) | 0.641 |
[i] CAD: coronary artery disease; OR: odds ratio; 95% CI: 95% confidence interval.
a Control group is the reference category.
Table 4
The baseline distribution characteristics of MGP alleles and their correlation with serum MGP concentrations. (Values are presented as mean ± SD.)
| Parameters | rs1800802 | rs4236 | rs12304 | sMGP (μg/L) | |||
|---|---|---|---|---|---|---|---|
| TT+TT vs. CC | p Valuea | Thr/Thr/Ala+Thr Ala/vs. Ala | p Valuea | Glu/Glu/Glu X+X/vs. X | p Valuea | Correlation | |
| Gender (F; M) | F:22; M: 30; F: 42; M: 74 | 0.452 | F: 9; M: 43; F: 40; M: 76 | 0.024 | F: 39; M: 12; F: 94; M:22 | 0.500 | 0.043 |
| LDL-cholesterol (mg/dL) | 106.81±38.62; 113.93±41.19 | 0.701 | 117.61±42.17; 108.47±39.26 | 0.394 | 112.15±37.40; 112.21±53.30 | 0.878 | 0.222 |
| HDL-cholesterol (mg/dL) | 44.69±16.78; 43.13±11.31 | 0.556 | 46.87±14.79; 41.72±11.76 | 0.049 | 44.29±8.53; 41.25±8.53 | 0.361 | 0.145 |
| Total cholesterol (mg/dL) | 188.28±46.01; 185.56±51.14 | 0.344 | 195.10±45.91; 181.37±51.04 | 0.245 | 187.45±48.46; 185.10±53.94 | 0.939 | 0.085 |
| Triglycerides (mg/dL) | 170.81±83.15; 166.67±102.21 | 0.769 | 177.05±119.29; 162.67±81.21 | 0.528 | 162.09±84.13; 199.42±139.30 | 0.115 | 0.903 |
| Fasting glucose (mg/dL) | 121.31±42.67; 117.13±42.46 | 0.544 | 125.54±51.81; 114.28±35.63 | 0.539 | 117.80±39.23; 121.63±56.52 | 0.653 | 0.588 |
| eGFR (mL/min./1.73m3) | 85.5786.75±±23.6220.99 ; | 0.835 | 88.8085.81±±22.0121.97 ; | 0.405 | 85.9888.70±±21.3724.28 ; | 0.377 | 0.015 |
[i] sMGP, serum MGP; eGFR, estimated glomerular filtration rate.
a Adjusted for age and gender status.
Figure 3
Linkage disequilibrium block structures of MGP gene polymorphisms was performed by Haploview (version 4.2) [12]. Numbers in squares (D’ values) indicate the pairwise correlation between polymorphisms. Color scheme from red to white represent higher to lower LD values.