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Increased expression of pentraxin 3 in placental tissues from patients with unexplained recurrent pregnancy loss Cover

Increased expression of pentraxin 3 in placental tissues from patients with unexplained recurrent pregnancy loss

Balkan Journal of Medical Genetics
Volume 22 (2019): Issue 1 (June 2019)
By: S Zeybek,  E Tepeli,  GO Cetin,  V Caner,  H Senol,  B Yildirim and  G Bagci  
Open Access
|Aug 2019

Figures & Tables

Figure 1

Mean PTX3 mRNA expression normalized for β-actin mRNA expression in the placentai tissues from URPL patients and control subjects. The small circles mean the outlier values and the asterisks (*) indicate extreme values.

Figure 2

Mean PTX3 mRNA expression normalized for β-actin mRNA expression in the placentai tissues from URPL patients without live birth, all women (URPL patients and controls) with previous live births. The small circles mean the outlier values and the asterisks (*) indicate extreme values.

Figure 3

Representative images of PTX3 immunhistochemistry staining in the placentai tissues derived from control subjects (a and b) and URPL patients (c and d). Pentraxin 3 is exclusively expressed in the cytoplasm. Positive staining is shown in brown. Subjects were semi-quantified as score 1 (a), score 2 (b), score 3 (c), score 4 (d) (original magnification, X400). (e) Immunhistochemistry scores for PTX3 expression was significantly higher in URPL patients than the control subjects. Values are expressed as means ± SDs.

Primer sequences and probes_

GenePrimer Sequences (5'>3')UPL ProbeAmplicon size
PTX3

F: GCTG GTG CTA GAG GAG CTG

R: GGA ATA AAA TAG CTG TTT CAC AAC CT

Probeno: #23 (04686977001)106 bp
β-actin

F: CGA CAG GAT GCA GAA GGA G

R: AGG AGG AGC AAT GAT CTT GAT CT

Probeno: #37 (04687957001)74 bp

Unexplained recurrent pregnancy loss and control group characteristics_

ParametersMean ± SDMean Values (min-max)Mean ± SDMean Values (min-max)ρ Value
Age (years)29.76 ±5.0429 (22-40)29.34 ±4.5129 (19-41)0.819
Number of previous miscarriages2.34 + 0.772(2-5)–––
Gestational week of current miscarriage10.16 ±3.589 (5-20)–––
Number of previous live births0.46 ± 0.610(0-2)1.68 ±0.742(1-4)0.0001
PTX3 mRNA expression levels0.0014 ±0.00590.0001 (0.000-0.418)0.000012 ±0.0000380.000002 (0.000-0.000264)0.0001
DOI: https://doi.org/10.2478/bjmg-2019-0002 | Journal eISSN: 2199-5761
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Language: English
Page range: 21 - 28
Published on: Aug 28, 2019
Published by: Macedonian Academy of Sciences and Arts
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Inflammation,
Pentraxin 3 (PTX3),
Placentai expression,
Real-time polymerase chain reaction (RT-PCR),
Unexplained recurrent pregnancy loss (URPL)
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2019 S Zeybek, E Tepeli, GO Cetin, V Caner, H Senol, B Yildirim, G Bagci, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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