Figure 1
Figure 2
Figure 3
Figure 4
Intergroup paired comparison in ΔCt = (CtTg-CtGAPDH) values_
| Mann-Whitney U Test | TCsa/TCbb | TCsa/TCrc | TCsa/HCd | TCbb/TCrc | TCbb/HCd | TCrc/HCd |
|---|---|---|---|---|---|---|
| Z value | –1.166 | –1.964 | –2.453 | –0.402 | –0.442 | 0.148 |
| Asymptotic significance (two-tailed) | 0.243 | 0.049e | 0.014e | 0.688 | 0.658 | 0.882 |
Fold change in expression of TSHR and Tg target genes between TCs and HC/TCr groups and between TCb and HC/TCr groups_
| Fold Change | For TSHR-mRNA | For Tg-mRNA |
|---|---|---|
| ΔΔCt = ΔCt (TCs)-ΔCt (HC) 2–ΔΔCt | –3.09904 (8.57) | –2.72137 (6.6) |
| ΔΔCt = ΔCt (TCs)-ΔCt (TCr) 2–ΔΔCt | –2.42523 (5.37) | –3.06057 (8.34) |
| ΔΔCt = ΔCt (TCb)-ΔCt (HC) 2–ΔΔCt | –3.82478 (14.17) | –0.61184 (1.53) |
| ΔΔCt = ΔCt (TCb)-ΔCt (TCr) 2–ΔΔCt | –3.15097 (8.88) | –0.95104 (1.93) |
Intergroup paired comparison in ΔCt = (CtTSHR-CtGAPDH) values_
| Mann-Whitney U Test | TCsa/TCbb | TCsa/TCrc | TCsa/HCd | TCbb/TCrc | TCbb/HCd | TCrc/HCd |
|---|---|---|---|---|---|---|
| Z value | 0.382 | –2.231 | –3.416 | –2.341 | –2.803 | –1.757 |
| Asymptotic significance (two-tailed) | 0.703 | 0.026e | 0.0006e | 0.019e | 0.005e | 0.079 |
Two-group comparison for Ct = (CtTg-CtGAPDH) values_
| Groups | Mean | n | SD | Minimum | Maximum | Percentiles | ||
|---|---|---|---|---|---|---|---|---|
| 25th | 50th (median) | 75th | ||||||
| TCsa+TCbb | 6.81 | 27 | 4.91 | –5.49 | 15.00 | 4.915 | 8.35 | 10.17 |
| TCrc+HDd | 9.82 | 27 | 2.53 | 5.71 | 15.45 | 8.14 | 9.62 | 10.85 |
| Summary | 8.32 | 54 | 4.16 | –5.49 | 15.45 | 7.23 | 8.86 | 10.37 |
Four intergroup comparisons for ΔCt = (CtTSHR-CtGAPDH) values_
| Groups | Mean | n | SD | Minimum | Maximum | Percentiles | ||
|---|---|---|---|---|---|---|---|---|
| 25th | 50th (median) | 75th | ||||||
| TCsa | 9.62 | 19 | 2.66 | 4.66 | 13.13 | 7.47 | 9.58 | 12.26 |
| TCbb | 8.89 | 6 | 3.26 | 4.15 | 12.55 | 5.63 | 10.13 | 10.77 |
| TCrc | 12.04 | 12 | 1.76 | 10.59 | 17.21 | 11.06 | 11.55 | 12.29 |
| HCd | 12.72 | 15 | 1.65 | 10.36 | 17.00 | 11.39 | 12.66 | 13.57 |
| Summary | 10.99 | 52 | 2.70 | 4.15 | 17.21 | 10.13 | 11.21 | 12.57 |
Four-group comparison for ΔCt = (CtTg-CtGAPDH) values_
| Groups | Mean | n | SD | Minimum | Maximum | Percentiles | ||
|---|---|---|---|---|---|---|---|---|
| 25th | 50th (median) | 75th | ||||||
| TCsa | 6.17 | 21 | 5.37 | –5.49 | 15.00 | 2.74 | 7.79 | 9.40 |
| TCbb | 9.07 | 6 | 1.55 | 7.22 | 10.61 | 7.39 | 9.36 | 10.47 |
| TCrc | 10.02 | 11 | 2.60 | 6.01 | 15.45 | 7.82 | 9.62 | 12.17 |
| HCd | 9.68 | 16 | 2.28 | 5.71 | 13.85 | 8.81 | 9.69 | 10.64 |
| Summary | 8.32 | 54 | 4.16 | 5.49 | 15.45 | 7.23 | 8.86 | 10.37 |
Two-group comparison for ΔCt = (TSHR-GAPDH) values_
| Groups | Mean | n | SD | Minimum | Maximum | Percentiles | ||
|---|---|---|---|---|---|---|---|---|
| 25th | 50th (median) | 75th | ||||||
| TCsa+TCbb | 9.44 | 25 | 2.76 | 4.15 | 13.13 | 7.465 | 9.94 | 11.66 |
| TCrc+HCd | 12.42 | 27 | 1.70 | 10.36 | 17.21 | 11.20 | 12.05 | 13.33 |
| Summary | 10.99 | 52 | 2.70 | 4.15 | 17.21 | 10.12 | 11.21 | 12.57 |