Protective role of Moringa oleifera seed nanoparticles against lipid metabolism disturbances, oxidative stress, and immune dysregulation in diet-induced hyperlipidemic Wistar rats

Muhammad Naeem; Ahmed J. Ahmed; Husni Abdulla Mhammad; Basim S. A. Al Sulivany; Bakhtyar Nader Ali

doi:10.2478/azibna-2026-0004

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Protective role of Moringa oleifera seed nanoparticles against lipid metabolism disturbances, oxidative stress, and immune dysregulation in diet-induced hyperlipidemic Wistar rats

Archiva Zootechnica

Volume 29 (2026): Issue 1 (June 2026)

By: Muhammad Naeem, Ahmed J. Ahmed, Husni Abdulla Mhammad, Basim S. A. Al Sulivany and Bakhtyar Nader Ali

Open Access

|Jun 2026

Abstract

This study investigated the protective effects of Moringa oleifera seed nanoparticles (MO-SeNPs) against high-fat diet-induced hyperlipidemia in Wistar rats. Rats were allocated into normal control, high-fat diet (HC) control, and HC groups receiving MO-SeNPs (25, 100, or 200 mg/kg) for 60 days. The HC group showed significant dyslipidemia, oxidative stress (elevated MDA, decreased SOD/CAT), and immune dysregulation (increased TNF-α/IL-6, decreased IL-10). MO-SeNP supplementation, particularly at 200 mg/kg, dose-dependently reversed these alterations, restoring lipid profiles, oxidative balance, and cytokine levels to near-normal. Results demonstrate that MOSeNPs effectively ameliorate hyperlipidemia-induced metabolic disturbances, highlighting their potential as a natural therapeutic agent.

References

Adisa IO, Pullagurala VLR, Peralta-Videa JR, Dimkpa CO, Elmer WH, Gardea-Torresdey JL, White JC. 2019. Recent advances in nano-enabled fertilizers and pesticides: a critical review of mechanisms of action. Environment Scie: Nano, 6(7), pp. 2002-2030.
Search in Google Scholar Back to article
Aebi H. 1984. Catalase in vivo. Methods in Enzymol. Oxygen Radicals in Biological Systems, 105, pp. 121-126.
Search in Google Scholar Back to article
Ahmed, B., Mustafa, M., Albassam, N., Yousif, A., Al-Taee, N. T. T. A., Saeed, K., Hassan, A., Owais, M. Acute and Chronic Fluoride Exposure Impairs Blood Physiology, Immunity, and Metabolic Disruptions in Labeo rohita. Egyptian Journal of Aquatic Biology and Fisheries, 2025; 29(5), pp, 2429-2448.
Search in Google Scholar Back to article
Al Sulivany B, Hoseini S. 2025. Pre-Exposure of Common Carp to Ambient Copper and Microplastic Changes the Gill Ionoregulaion-Related Transcripts During Saltwater Exposure. Aqua Scie. Fis Resour, 6(1), pp, 39-51.
Search in Google Scholar Back to article
Al Sulivany BSA, Omar I, Yousif A, Owais M. Effects of Dietary Green Microalgae (Chlorella vulgaris) and Iron Nanoparticles on Biochemical, Enzymatic, and Tissue Health in Cyprinus carpio. Journal. Aquaculture. Science. [Internet]. 2025 Sep. 30 [cited 2025 Oct. 29];10(2), pp, 98-108.
Search in Google Scholar Back to article
Alkhudhayri DA, Osman MA, Alshammari GM, Al Maiman SA, Yahya MA. 2021. Moringa peregrina leaf extracts produce anti-obesity, hypoglycemic, anti-hyperlipidemic, and hepatoprotective effects on high-fat diet-fed rats. Saudi J. Bio. Scie, 28(6), pp. 3333-3342.
Search in Google Scholar Back to article
Allain CC, Poon LS, Chan CS, Richmond WF, Fu PC. 1974. Enzymatic determination of total serum cholesterol. Clinical chemistry, 20(4), pp.470-475.
Search in Google Scholar Back to article
Ashfaq R, Rasul A, Asghar S, Kovács A, Berkó S, Budai-Szűcs M. 2023. Lipid nanoparticles: an effective tool to improve the bioavailability of nutraceuticals. Int. J Mol. Sci, 24(21), pp. 15764.
Search in Google Scholar Back to article
Banerjee A, Das D, Paul R, Roy S, Bhattacharjee A, Prasad S K, Maji B K. 2020. Altered composition of high-lipid diet may generate reactive oxygen species by disturbing the balance of antioxidants and free radicals. J. Bas. Clinic and pharmacol, 31(3), pp. 20190141.
Search in Google Scholar Back to article
Bechmann LP, Hannivoort RA, Gerken G, Hotamisligil GS, Trauner M, Canbay A. 2012. The interaction of hepatic lipid and glucose metabolism in liver diseases. J. Hepato, 56(4), pp. 952-964.
Search in Google Scholar Back to article
Buettner R, Schölmerich J, Bollheimer LC. 2007. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obes, 15(4), pp. 798-808.
Search in Google Scholar Back to article
Chhikara N, Kaur A, Mann S, Garg MK, Sofi SA, Panghal A. 2021. Bioactive compounds, associated health benefits, and safety considerations of Moringa oleifera L.: An updated review. Nut. Foo Scie, 51(2), pp. 255-277.
Search in Google Scholar Back to article
Dinarello CA. 2000. Proinflammatory cytokines. Chest, 118(2), pp. 503-508.
Search in Google Scholar Back to article
El-Demerdash FM, Nasr HM. 2014. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J. of Tra. Elem. in Med. Bio, 28(1), pp. 89-93.
Search in Google Scholar Back to article
Fazal RM, Fatima A, Al Sulivany BSA, Hussain R, Hassan A, Shahid A, Ashiq K, Owais, M. 2025. Enhancing Growth Performance, Antioxidant Defense, and Immune Response in Striped Catfish (Pangasius Hypophthalmus) Through Dietary Supplementation with Aloe vera: A Sustainable Aquaculture Approach. Sci J. Univer Zakh, 13(4), pp, 510–518.
Search in Google Scholar Back to article
Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinic Chem, 18(6), pp.499-502.
Search in Google Scholar Back to article
Gabay C, Kushner I. 1999. Acute-phase proteins and other systemic responses to inflammation. New Eng. J. medicine, 340(6), pp. 448-454.
Search in Google Scholar Back to article
Gangavarapu A, Tapia-Lopez LV, Sarkar B, Pena-Zacarias J, Badruddoza AZM, Nurunnabi M. 2024. Lipid nanoparticles for enhancing oral bioavailability. Nanoscal, 16(39), pp. 18319-18338.
Search in Google Scholar Back to article
García RD, Asensio JA, Perdicaro DJ, de los angeles, Peral M. 2022. The role of inflammation as a preponderant risk factor in cardiovascular diseases. Curr Vasc Pharmacol, 20(3), pp. 244-259.
Search in Google Scholar Back to article
Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. 1977. High-density lipoprotein as a protective factor against coronary heart disease: the Framingham Study. Amer J. Med, 62(5), pp. 707-714.
Search in Google Scholar Back to article
Guo Q, Jin Y, Chen X, Ye X, Shen X, Lin M, Zhang J. 2024. NF-κB in biology and targeted therapy: new insights and translational implications. Signal transduction and targeted therapy, 9(1), pp. 53.
Search in Google Scholar Back to article
Herrington W, Lacey B, Sherliker P, Armitage J, Lewington S. 2016. Epidemiology of atherosclerosis and the potential to reduce the global burden of atherothrombotic disease. Circul resea, 118(4), pp. 535-546.
Search in Google Scholar Back to article
Hussein J, El-Bana M, Abdel-Latif Y, El-Sayed S, Shaarawy S, Medhat D. 2024. Moringa oleifera leaves extract-loaded gold nanoparticles offer a promising approach in protecting against experimental nephrotoxicity. Prost. Oth. Lip Med, 170, pp. 106800.
Search in Google Scholar Back to article
Islam MA, Medha MM, Nahar AU, Al Fahad MA, Siraj MA, Seidel V. 2023. Cancer protective role of selected dietary polyphenols via modulating Keap1/Nrf2/ARE and interconnected signaling pathways. Nutr & Canc, 75(4), pp. 1065-1102.
Search in Google Scholar Back to article
Karanovic D, Mihailovic-Stanojevic N, Miloradovic Z, Ivanov M, Vajic UJ, Grujic-Milanovic J, Jovovic D. (2021). Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis. Phyto Resea, 35(3), pp.1534-1545.
Search in Google Scholar Back to article
Karnwal A, Jassim AY, Mohammed AA, Sharma V, Al-Tawaha AR, Sivanesan I. 2024. Nanotechnology for healthcare: plant-derived nanoparticles in disease treatment and regenerative medicine. Pharmaceut, 17(12), pp. 1711.
Search in Google Scholar Back to article
Kashyap P, Kumar S, Riar CS, Jindal N, Baniwal P, Guiné RP, Kumar H. 2022. Recent advances in Drumstick (Moringa oleifera) leaves bioactive compounds: Composition, health benefits, bioaccessibility, and dietary applications. Antioxi, 11(2), pp. 402-421.
Search in Google Scholar Back to article
Kukreti N, Chitme HR, Varshney VK, Abdel-Wahab BA, Khateeb MM, Habeeb MS. 2023. Antioxidant properties mediate nephroprotective and hepatoprotective activity of essential oil and hydro-alcoholic extract of the high-altitude plant Skimmia anquetilia. Antioxidants, 12(6), pp. 1167-1175.
Search in Google Scholar Back to article
Marini BL, Choi SW, Byersdorfer CA, Cronin S, Frame DG. 2015. Treatment of dyslipidemia in allogeneic hematopoietic stem cell transplant patients. Biol. Blo. & Marr Transpl, 21(5), pp. 809-820.
Search in Google Scholar Back to article
Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Euro. J Bio, 47(3), pp.469-474.
Search in Google Scholar Back to article
Mastino P, Rosati D, de Soccio G, Romeo M, Pentangelo D, Venarubea S, Minni A. 2023. Oxidative stress in obstructive sleep apnea syndrome: putative pathways to hearing system impairment. Antioxi, 12(7), pp. 1430-1439.
Search in Google Scholar Back to article
Matyas C, Haskó G, Liaudet L, Trojnar E, Pacher P. 2021. Interplay of cardiovascular mediators, oxidative stress and inflammation in liver disease and its complications. Nat. Revi Cardiol, 18(2), pp. 117-135.
Search in Google Scholar Back to article
Mohammadi N, Farrell M, O’Sullivan L, Langan A, Franchin M, Azevedo L, Granato D. 2024. Effectiveness of anthocyanin-containing foods and nutraceuticals in mitigating oxidative stress, inflammation, and cardiovascular health-related biomarkers: a systematic review of animal and human interventions. Foo & Func, 15(7), pp. 3274-3299.
Search in Google Scholar Back to article
Mthiyane FT, Dludla PV, Ziqubu K, Mthembu SX, Muvhulawa N, Hlengwa N, Mazibuko-Mbeje SE. 2022. A review on the antidiabetic properties of Moringa oleifera extracts: focusing on oxidative stress and inflammation as main therapeutic targets. Front Pharmacol, 13, pp. 940572.
Search in Google Scholar Back to article
Namiq K, Albassam NH, Wahab NK. Assessment of Pesticide Residues in Fish Tissues and Water at Various Times of the Year. DASJ, 17(1), pp, 178-93.
Search in Google Scholar Back to article
Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytic Biochem, 95(2), pp. 351-358.
Search in Google Scholar Back to article
Owais M, AL Sulivany BSA, Gali Romani FAM, FAZAL RM, Ashraf S. 2025. Morphometry, dietary composition, and feeding patterns of Clarius gariepinus from the Chenab River, Punjab, Pakistan. Iran. J. Ichthyol. 12(2), pp, 147-61.
Search in Google Scholar Back to article
Pappachan JM, Babu S, Krishnan B, Ravindran NC. 2017. Non-alcoholic fatty liver disease: a clinical update. J. Clin. Trans. Hepat, 5(4), pp. 384-496.
Search in Google Scholar Back to article
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Bio & Med, 26(9-10), pp. 1231-1237.
Search in Google Scholar Back to article
Sabat S, Bej S, Swain S, Bishoyi AK, Sahoo CR, Sabat G, Padhy RN. 2025. Phycochemistry and pharmacological significance of filamentous cyanobacterium Spirulina sp. Biores Bioproces, 12(1), pp. 27-39
Search in Google Scholar Back to article
Sailaja BS, Aita R, Maledatu S, Ribnicky D, Verzi MP, Raskin I. 2021. Moringa isothiocyanate-1 regulates Nrf2 and NF-κB pathway in response to LPS-driven sepsis and inflammation. PLoS One, 16(4), pp. e0248691.
Search in Google Scholar Back to article
Samar J, Butt GY, Shah AA, Shah, AN, Ali S, Jan BL, Hussaan, M. 2022. Phycochemical and biological activities from different extracts of Padina antillarum (Kützing) Piccone. Front. Plan Scie, 13, pp. 929368.
Search in Google Scholar Back to article
Sedlak J, Lindsay RH. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analy Bio, 25, pp. 192-205.
Search in Google Scholar Back to article
Shahbaz M, Naeem H, Batool M, Imran M, Hussain M, Mujtaba A, Al Jbawi E. 2024. Antioxidant, anticancer, and anti-inflammatory potential of Moringa seed and Moringa seed oil: A comprehensive approach. Foo Scie. Nutr, 12(9), pp. 6157-6173.
Search in Google Scholar Back to article
Siddique NA, AL-Samman AM, Kahkashan. 2024. GC–MS analysis and green synthesis of silver nanoparticles using Emblica officinalis leaves and evaluation of its antioxidant and in-vitro anti-hypercholesterolemic potential. J. Taib Uni. Scie, 18(1), pp. 2390211.
Search in Google Scholar Back to article
Singh D, Khan MA, Siddique HR. 2024. Unveiling the therapeutic promise of natural products in alleviating drug-induced liver injury: Present advancements and future prospects. Phytothe. Res, 38(1), pp. 22-41.
Search in Google Scholar Back to article
Tiwari R, Latheef SK, Ahmed I, Iqbal HM, Bule MH, Dhama K. Farag MR. 2018. Herbal immunomodulators-a remedial panacea for designing and developing effective drugs and medicines: current scenario and future prospects. Curr. Dru. Metab, 19(3), pp. 264-301.
Search in Google Scholar Back to article
Wahi A, Bishnoi M, Raina N, Singh MA, Verma P, Gupta PK, Gupta, M. 2023. Recent updates on nano-phyto-formulations based therapeutic intervention for cancer treatment. Oncol Rese, 32(1), pp. 19-28.
Search in Google Scholar Back to article
Xu Y, Jia X, Zhang W, Xie Q, Zhu M, Zhao Z, Li H. 2023. The effects of Ascophyllum nodosum, Camellia sinensis-leaf extract, and their joint interventions on glycolipid and energy metabolism in obese mice. Front Nut, 10, pp. 1242157.
Search in Google Scholar Back to article
Yao YS, Li TD, Zeng ZH. 2020. Mechanisms underlying direct actions of hyperlipidemia on myocardium: an updated review. Lip in Heal & Dise, 19(1), pp. 23-37.
Search in Google Scholar Back to article
Zakaria Z, Othman ZA, Nna VU, Mohamed M. 2023. The promising roles of medicinal plants and bioactive compounds on hepatic lipid metabolism in the treatment of non-alcoholic fatty liver disease in animal models: molecular targets. Arch. Physio & Biochem, 129(6), pp. 1262-1278.
Search in Google Scholar Back to article
Zhang Y, Zhang XY, Shi SR, Ma CN, Lin YP, Song WG, Guo SD. 2024. Natural products in atherosclerosis therapy by targeting PPARs: A review focusing on lipid metabolism and inflammation. Front Card Med, 11, pp. 1372055.
Search in Google Scholar Back to article
Zulfiqar R, Asad F, Al Sulivany BSA, Naseer S, Kanwal I, Nadeem A, Owais M. (2025). Brown seaweed (sargassum ilicifolium) as a nutritional supplement: effects on growth and hematology in labeo rohita. SJUOZ.13(2), pp, 166-171.
Search in Google Scholar Back to article

Articles in this issue

DOI: https://doi.org/10.2478/azibna-2026-0004 | Journal eISSN: 2344-4592 | Journal ISSN: 1016-4855

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Language: English

Page range: 58 - 772

Published on: Jun 20, 2026

Published by: National Institute for Research-Development in Biology and Animal Nutrition

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Moringa oleifera,

lipid metabolism,

oxidative stress,

inflammatory responses,

hyperlipidemic Wistar rats

Related subjects:

Life sciences,

Life sciences, other

© 2026 Muhammad Naeem, Ahmed J. Ahmed, Husni Abdulla Mhammad, Basim S. A. Al Sulivany, Bakhtyar Nader Ali, published by National Institute for Research-Development in Biology and Animal Nutrition
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 29 (2026): Issue 1 (June 2026)