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Oxolinic acid therapy: An effective way to reduce Aeromonas veronii infection in Oreochromis niloticus and improve biochemical and hematological parameters and histopathological lesions Cover

Oxolinic acid therapy: An effective way to reduce Aeromonas veronii infection in Oreochromis niloticus and improve biochemical and hematological parameters and histopathological lesions

Open Access
|May 2025

References

  1. Abraham, T. J., Patel, J. B., Bardhan, A., Rajisha, R., Panda, S. K., Patil, P. K. (2024). Safety, tolerability and biological responses of Oreochromis niloticus juveniles upon oral oxolinic acid administration. Journal of Veterinary Pharmacology and Therapeutics, 47(2), 121-133.
  2. Bardhan, A., Abraham, T. J., Qureshi, Q. A., Chakraborty, R. (2021). Antibiotic-resistant motile aeromonads associated with cultured Indian major carps, pond water and pond sediment. Bacterial Empire, 4(4), e310-e316.
  3. Bardhan, A., Abraham, T. J., Singha, J., Saha, S., Sarker, S., Patil, P. K. (2022). The effects of extended feeding of florfenicol-coated medicated diets on the safety, serum biomarkers and blood cells morphology of Nile tilapia Oreochromis niloticus (L.). Environmental Science and Pollution Research, 29(26), 39914-39927.
  4. Bearden, D. T., Danziger, L. H. (2001). Mechanism of action of and resistance to quinolones. Pharmacotherapy. The Journal of Human Pharmacology and Drug Therapy, 21(10P2), 224S-232S.
  5. Bektas, S., Ayik, O. (2022). Alterations in some selected hematological parameters and glucose-6-phosphate dehydrogenase (G6PD) activity of rainbow trout (Oncorhynchus mykiss), experimentally infected with Aeromonas salmonicida. Pakistan Journal of Zoology, 54(5), 2119-2125.
  6. Bortolotte, A. R., Daniel, D., Reyes, F. G. R. (2020). Occur-rence of antimicrobial residues in tilapia Oreochromis niloticus fillets produced in Brazil and available at the retail market. Food Research International, 140, 109865.
  7. Bowker, J. D., Carty, D., Bowman, M. P. (2013). The safety of Aquaflor (50% florfenicol) administered in feed to fingerling yellow perch. North American Journal of Aquaculture, 75(4), 517-523.
  8. CLSI. (2024). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. In M07 Standard, 12th edn., Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA.
  9. Collins, C. H., Lynes, P. M., Grange, J. M. (2004). Collins and Lyne’s Microbiological Methods. 8th edn., Arnold publishers, London, 445p.
  10. Delalay, G., Berezowski, J. A., Diserens, N., Schmidt-Posthaus, H. (2020). An understated danger: Antimicrobial resistance in aquaculture and pet fish in Switzerland, a retrospective study from 2000 to 2017. Journal of Fish Diseases, 43(10), 1299-1315.
  11. Dien, L. T., Ngo, T. P. H., Nguyen, T. V., Kayansamruaj, P., Salin, K. R., Mohan, C. V., Rodkhum, C., Dong, H. T. (2023). Non-antibiotic approaches to combat motile Aeromonas infections in aquaculture: Current state of knowledge and future perspectives. Reviews in Aquaculture, 15(1), 333-366.
  12. DOF. (2022). Handbook on fisheries statistics. Fisheries Statistics Division, Department of Fisheries, Ministry of Fisheries, Animal Husbandry and Dairying, Government of India.
  13. Dong, H. T., Techatanakitarnan, C., Jindakittikul, P., Thaiprayoon, A., Taengphu, S., Charoensapsri, W., Charoensapsri, W., Khunrae, P., Rattanarojpong, T., Senapin, S. (2017). Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.). Journal of Fish Diseases, 40(10), 1395-1403.
  14. Elgendy, M. Y., Shaalan, M., Abdelsalam, M., Eissa, A. E., El-Adawy, M. M., Seida, A. A. (2022). Antibacterial activity of silver nanoparticles against antibiotic-resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay. Aquaculture Research, 53(3), 901-920.
  15. El-Gohary, F. A., Zahran, E., El-Gawad, A., Eman, A., El-Gohary, A. H., M Abdelhamid, F., El-Mleeh, A., Elmahallawy, E. K., Elsayed, M. M. (2020). Investigation of the prevalence, virulence genes, and antibiogram of motile aeromonads isolated from Nile tilapia fish farms in Egypt and assessment of their water quality. Animals, 10(8), 1432.
  16. El-Latif, A. A., Elabd, H., Amin, A., Eldeen, A. N., Shaheen, A. A. (2019). High mortalities caused by Aeromonas veronii: identification, pathogenicity, and histopathological studies in Oreochromis niloticus. Aquaculture International, 27, 1725-1737.
  17. EMEA. (2005). Committee for medicinal products for veterinary use: oxolinic acid (Extension to all food-producing species). European Medicines Agency. Available at: https://www.ema.europa.eu/en/documents/committee-report/committee-medicinal-products-veterinary-use-cvmp-monthly-report-application-procedures-guidelines_en-4.pdf. Accessed 08 Dec 2023.
  18. FAO. (2024). The state of world fisheries and aquaculture 2024 - Blue Transformation in Action. Rome. https://doi.org/10.4060/cd0683en. Accessed 08 Dec 2023.
  19. Ferdous, F., Scott, T. R. (2015). A comparative examination of thrombocyte/platelet immunity. Immunology Letters, 163(1), 32-39.
  20. Ferri, G., Lauteri, C., Vergara, A. (2022). Antibiotic resistance in the finfish aquaculture industry: a review. Antibiotics, 11(11), 1574.
  21. Gao, S., Zhao, N., Amer, S., Qian, M., Lv, M., Zhao, Y., Su, X., Cao, J., He, H., Zhao, B. (2013). Protective efficacy of PLGA microspheres loaded with a divalent DNA vaccine encoding the ompA gene of Aeromonas veronii and the hly gene of Aeromonas hydrophila in mice. Vaccine, 31(48), 5754-5759.
  22. Haenen, O. L. M., Dong, H. T., Hoai, T. D., Crumlish, M., Karunasagar, I., Barkham, T., Chen, S. L., Zadoks, R., Kiermeier, A., Wang, B., Gamarro, E. G., Takeuchi, M., Azmai, M. N. A., Fouz, B., Pakingking, P., Wei, Z. W., Bondad-Reantaso, M. G. (2023). Bacterial diseases of tilapia, their zoonotic potential and risk of antimicrobial resistance. Reviews in Aquaculture, 15, 154-185.
  23. Hal, A. M., El-Barbary, M. I. (2021). Therapeutic effect of Nigella sativa oil and ciprofloxacin against bacterial infection based on interleukin 1â expression and kidney histopathological alterations in Oreochromis niloticus. Aquaculture Research, 52(6), 2772-2782.
  24. Harikrishnan, R., Rani, M. N., Balasundaram, C. (2003). Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture, 221, 41-50.
  25. Hassan, M. A., Noureldin, E. A., Mahmoud, M. A., Fita, N. A. (2017). Molecular identification and epizootiology of Aeromonas veronii infection among farmed Oreochromis niloticus in Eastern Province, KSA. The Egyptian Journal of Aquatic Research, 43(2), 161-167.
  26. Husien, M. M., Younis, A. A. (2000). Efficacy and residues of oxolinic acid in Oreochromis niloticus fish infected with enteric red mouth disease (ERM). Egyptian Journal of Agricultural Research, 78(1), 399-409.
  27. Julinta, R. B., Abraham, T. J., Roy, A., Singha, J., Dash, G., Nagesh, T. S., Patil, P. K. (2017). Histopathology and wound healing in oxytetracycline treated Oreochromis niloticus (L.) against Aeromonas hydrophila intramuscular challenge. Journal of Aquaculture Research and Development, 8, 488.
  28. Junge, W., Wilke, B., Halabi, A., Klein, G. (2004). Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffe method. International Journal of Clinical Chemistry, 344(1-2), 137-148.
  29. Kalowski, S., Kincaid-Smith, P., Pavillard, R. (1979). Oxolinic acid in the treatment of urinary tract infections. Medical Journal of Australia, 1(8), 345-347.
  30. Khan, M. I. R., Panda, K. (2023). Co-infection of multidrug-resistant Aeromonas hydrophila and A. veronii in pangas (Pangasianodon hypophthalmus) reared in cage culture units of Sarodha Reservoir, Kawardha, Chhattisgarh, India – An outbreak report. Indian Journal of Animal Health, 62(1), 217-221.
  31. Limbu, S. M., Chen, L. Q., Zhang, M. L., Du, Z. Y. (2021). A global analysis on the systemic effects of antibiotics in cultured fish and their potential human health risk: a review. Reviews in Aquaculture, 13(2), 1015-1059.
  32. Liu, G., Li, J., Jiang, Z., Zhu, X., Gao, X., Jiang, Q., Wang, J., Wei, W., Zhang, X. (2022). Pathogenicity of Aeromonas veronii causing mass mortalities of Odontobutis potamophila and its induced host immune response. Fish and Shellfish Immunology, 125, 180-189.
  33. Lulijwa, R., Rupia, E. J., Alfaro, A.C. (2020). Antibiotic use in aquaculture, policies and regulation, health and environmental risks: a review of the top 15 major producers. Reviews in Aquaculture, 12(2), 640-663.
  34. Michaylova, V., Ilkova, P. (1971). Photometric determination of micro amounts of calcium with arsenazo III. Analytica Chimica Acta, 53(1), 194-198.
  35. Okocha, R.C., Olatoye, I.O., Adedeji, O.B. (2018). Food safety impacts of antimicrobial use and their residues in aquaculture. Public Health Reviews, 39(1), 1-22.
  36. Patel, J. B., Abraham, T. J., Bardhan, A., Das, R., Sen, A., Boda, S., Patil, P. K. (2024). Efficacy of oxolinic acid against Aeromonas hydrophila infection in Nile tilapia Oreochromis niloticus juveniles. Aquaculture Studies, 24(3), AQUAST1653.
  37. Pessoa, R. B. G., de Oliveira, W. F., Marques, D. S. C., dos-Santos-Correia, M. T., de-Carvalho, E. V. M. M., Coelho, L.C.B.B. (2019). The genus Aeromonas: A general approach. Microbial Pathogenesis, 130, 81-94.
  38. Pessoa, R. B. G., de Oliveira, W. F., Correia, M. T. D. S., Fontes, A., Coelho, L. C. B. B. (2022). Aeromonas and human health disorders: clinical approaches. Frontiers in Microbiology, 13, 868890.
  39. Quesada, S. P., Paschoal, J. A. R., Reyes, F. G. R. (2013). Considerations on the aquaculture development and the use of veterinary drugs: special issue for fluoroquinolones -A review. Journal of Food Science, 78(9), 1321-1333.
  40. Raj, N. S., Swaminathan, T. R., Dharmaratnam, A., Raja, S. A., Ramraj, D., Lal, K. K. (2019). Aeromonas veronii caused bilateral exophthalmia and mass mortality in cultured Nile tilapia Oreochromis niloticus (L.) in India. Aquaculture, 512, 734278.
  41. Reda, R., El-Murr, A., Abd Elhakim, Y., El-Shahat, W. (2021). Relationship between the productivity losses of tilapia and Aeromonas veronii infection. Zagazig Veterinary Journal, 49(2), 124-142.
  42. Reda, R. M., El-Murr, A., Abd Elhakim, Y., El-Shahat, W. (2022). Aeromonas veronii detection in Egyptian fish farms with summer tilapia mortality outbreaks and the role of formic acid in limiting its spread. Aquaculture Research, 53(3), 940-956.
  43. Reed, L. J., Muench, H. (1938). A simple method of estimating fifty per cent endpoints. American Journal of Epidemiology, 27(3), 493-497.
  44. Rey, A., Verján, N., Ferguson, H. W., Iregui, C. (2009). Pathogenesis of Aeromonas hydrophila strain KJ99 infection and its extracellular products in two species of fish. Veterinary Record, 164(16), 493-499.
  45. Richardson, R., Slanchev, K., Kraus, C., Knyphausen, P., Eming, S., Hammerschmidt, M. (2013). Adult zebrafish as a model system for cutaneous wound-healing research. Journal of Investigative Dermatology, 133(6), 1655-1665.
  46. Rigos, G., Alexis, M., Tyrpenou, A. E., Nengas, I., Piper, I., Troisi, G. (2002). Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L. Journal of Fish Diseases, 25(7), 401-408.
  47. Roberts, R. J. (2012). Fish Pathology. John Wiley and Sons, USA.
  48. Roy, A., Abraham, T. J., Namdeo, M. S., Singha, J., Julinta, R. B., Boda, S. (2019). Effects of oral oxytetracycline-therapy on wound progression and healing following Aeromonas caviae infection in Nile tilapia (Oreochromis niloticus L.). Brazilian Archives of Biology and Technology, 62(1), e19180766.
  49. Saharia, P. K., Hussain, I. A., Pokhrel, H., Kalita, B., Borah, G., Yasmin, R. (2021). Prevalence of motile Aeromonas Septicaemia (MAS) in fish culture systems of the Central Brahmaputra Valley zone of Assam, India. Aquaculture Research, 52(3), 1201-1214.
  50. Said, A. A., Reda, R. M., Metwally, M. M., Abd El-Hady, H. M. (2023). Therapeutic efficacy of coriander (Coriandrum sativum) enriched diets in Oreochromis niloticus: effect on hepatic-renal functions, the antioxidant-immune response and resistance to Aeromonas veronii. Fish Physiology and Biochemistry, 49(4), 687-709.
  51. Samuelsen, O. B., Bergh, q. (2004). Efficacy of orally administered florfenicol and oxolinic acid for the treatment of vibriosis in cod (Gadus morhua). Aquaculture, 235(1-4), 27-35.
  52. Schmidt, H., Goetz, K., Hallmann, I., Holt, C. V. (1961). The course of resynthesis of blood glucose. Biochemical Journal, 334, 534-544.
  53. Schoenfeld, I. (1964). Direct spectrochemical determination of some rare earth elements in uranium. Israel Journal of Chemistry, 2(3), 99-104.
  54. Smyrli, M., Prapas, A., Rigos, G., Kokkari, C., Pavlidis, M., Katharios, P. (2017). Aeromonas veronii infection associated with high morbidity and mortality in farmed European seabass Dicentrarchus labrax in the Aegean Sea, Greece. Fish Pathology, 52(2), 68-81.
  55. Sreedharan, K., Philip, R., Singh, I. S. B. (2013). Characterization and virulence potential of phenotypically diverse Aeromonas veronii isolates recovered from moribund freshwater ornamental fishes of Kerala, India. Antonie Van Leeuwenhoek, 103, 53-67.
  56. Suresh, T., Nithin, M.S., Kushala, K.B., Girisha, S.K., Shivakumar, V.B., Dheeraj, S.B., Puneeth, T.G., Kishan, K., Vinay, T. N. (2023). Largescale mortality of Oreochromis mossambicus in lakes and reservoirs of Karnataka due to coinfection of Tilapia Lake virus (TiLV) and multidrug-resistant Aeromonas veronii: An emerging fish disease in India. Aquaculture, 565, 739077.
  57. Sveen, L. R., Timmerhaus, G., Krasnov, A., Takle, H., Handeland, S., Ytteborg, E. (2019). Wound healing in post-smolt Atlantic salmon (Salmo salar L.). Scientific Reports, 9(1), 3565.
  58. Thomassen, G. M. B., Reiche, T., Tennfjord, C. E., Mehli, L. (2022). Antibiotic resistance properties among Pseudo-monas spp. associated with salmon processing environments. Microorganisms, 10(7), 1420.
  59. Tietz, N. W. (1994). Accuracy in clinical chemistry–does anybody care? Clinical Chemistry, 40(6), 859-861.
  60. USFDA. (2023). Approved aquaculture drugs. U.S. Food and Drug Administration. https://www.fda.gov/animal-veterinary/aquaculture/approved-aquaculture--drugs. Accessed 08 Dec 2023.
  61. Vinoth, P., Manish, K. P., Dhasarathan, P. (2018). Wound healing mechanism in fish muscle tissue after administration of Coscinium fenestratum, Azadirachta indica, Cynodon dactylon plant extract. International Journal of Advance Research in Science and Engineering, 7(3), 1070-1077.
  62. Wei, K., Yin, Z., Xie, Y. (2016). Roles of the kidney in the formation, remodelling and repair of bone. Journal of Nephrology, 29(3), 349-357.
  63. WHO (2024). WHO list of medically important antimicrobials. World Health Organization, Antimicrobial Resistance Division, Geneva, Switzerland. https://cdn.who.int/mediadocs/default-source/gcp/who-mia-list-2024-lv.pdf?sfvrsn=3320dd3d_2. Accessed 08 Dec 2023.
  64. Wintrobe, M. M. (1946). Clinical Hematology. Wolters Kluwer, USA.
  65. WOAH. (2021). List of antimicrobial agents of veterinary importance. World Organisation for Animal Health, Paris, France. https://www.woah.org/app/uploads/2021/03/oie-list-antimicrobials.pdf. Accessed 08 Dec 2023.
  66. Wolf, P. L., Williams, D., Coplon, N., Coulson, A. S. (1972). Low aspartate transaminase activity in serum of patients undergoing chronic hemodialysis. Clinical Chemistry, 18(6), 567-568.
DOI: https://doi.org/10.2478/aopf-2025-0001 | Journal eISSN: 2545-059X | Journal ISSN: 2545-0255
Language: English
Page range: 1 - 18
Submitted on: Sep 2, 2024
|
Accepted on: Mar 6, 2025
|
Published on: May 23, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Priyanka Sinha, Thangapalam Jawahar Abraham, Arya Sen, Joshi Sharon, Ratnapriya Das, Arpan Ghorai, Satyanarayana Boda, Prasanna Kumar Patil, published by Stanisław Sakowicz Inland Fisheries Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.