Injectable hyaluronic acid: a method for accelerating alveolar bone remodelling during orthodontic tooth movement

Injectable hyaluronic acid: a method for accelerating alveolar bone remodelling during orthodontic tooth movement

Australasian Orthodontic Journal

Volume 40 (2024): Issue 2 (July 2024)

By:

Dler Mourad, Merve Goymen, Fatma Deniz Uzuner, Tuna Onal and Mehmet Ibrahim Tuglu

Open Access

|Feb 2025

Figures & Tables

3D model: A, The blue points refer to the superimposition points and measurements of molar rotation around its long axis. B, Measurement of orthodontic tooth movement, green point refer to T0, red point refer to T1.

Comparison of OTM amount in all time points between groups I and II (E): experimental, (C) control.

Histologic images of osteoclast cells in interradicular area of maxillary molar on day 14 and 21: A, GI experimental side on day 14. B, GII experimental side on day 14. C, Saline injected side onday 14. D, GI experimental side on day 21. E, GII experimental side on day 21. F, Saline injected side on day 21.

RANKL/OPG ratios at experimental sides of groups I and II in each observation days.

Histologic images of RANKL and OPG expression in interradicular area of maxillary molar on day 21: A, RANKL in GI experimental side. B, RANKL in GII experimental side. C, RANKL in Saline injected side. D, OPG in GI experimental side. E, OPG in GII experimental side. F, OPG in Saline injected side.

BV/TV Percentage in Experimental and Control Groups (Mean ± SD) (%)

Group	Experimental	Control	p
Group I- Day 1	47.254 ± 2.528	47.270 ± 2.205	NS
Group I- Day 3	43.458 ± 2.44	42.050 ± 1.522	NS
Group I- Day 7	29.164 ± 3.846	31.296 ± 3.828	NS
Group I- Day 14	25.594 ± 3.562	31.088 ± 3.222	0.05*
Group I- Day 21	32.111 ± 0.7840	39.890 ± 2.068	0.05*
Group II- Day 1	47.884 ± 2.340	45.846 ± 2.727	NS
Group II- Day 3	46.089 ± 3.447	46.168 ± 3.042	NS
Group II- Day 7	26.818 ± 3.910	27.026 ± 3.489	NS
Group II- Day 14	26.379 ± 3.757	32.386 ± 3.825	0.05*
Group II- Day 21	36.071 ± 2.632	39.459 ± 2.028	0.05*

RANKL/OPG Ratio in Study Group (Mean ± SD)

	Experimental	Control	p
Group I- Day 1	1.08 ± 0.09	1.14 ± 0.23	0.336
Group I- Day 3	1.14 ± 0.16	1.1 ± 0.23	0.387
Group I- Day 7	1.06 ± 0.25	1.01 ± 0.23	0.297
Group I- Day 14	1.17 ± 0.16	1.14 ± 0.18	0.044*
Group I- Day 21	1.45 ± 0.16	1.21 ± 0.15	0.03*
Group II- Day 1	1.01 ± 0.15	1.01 ± 0.15	0.481
Group II- Day 3	1.21 ± 0.27	1.05 ± 0.16	0.161
Group II- Day 7	1.2 ± 0.18	0.91 ± 0.12	0.021*
Group II- Day 14	1.28 ± 0.1	1.11 ± 0.14	0.043*
Group II- Day 21	1.37 ± 0.12	1.25 ± 0.18	0.048*

Comparison of tooth movements between experimental and control sides on each observational day (Mean ± SD) (mm)

Group	Experimental	Control	P
Group I- Day 1	0.046 ± 0.009	0.026 ± 0.01	0.002*
Group I- Day 3	0.469 ± 0.024	0.341 ± 0.028	0.001**
Group I- Day 7	0.67 ± 0.024	0.456 ± 0.028	0.001**
Group I- Day 14	0.989 ± 0.045	0.54 ± 0.047	0.001**
Group I- Day 21	1.143 ± 0.054	0.93 ± 0.048	0.001**
Group II- Day 1	0.033 ± 0.008	0.026 ± 0.007	0.06
Group II- Day 3	0.45 ± 0.039	0.346 ± 0.026	0.001**
Group II- Day 7	0.637 ± 0.039	0.444 ± 0.026	0.001**
Group II- Day 14	0.964 ± 0.067	0.552 ± 0.056	0.001**
Group II- Day 21	0.977 ± 0.035	0.866 ± 0.037	0.001**

Number of Osteoclasts in Study Groups (Mean ± SD) (+1/+5 Score)

	Experimental	Control	p
Group I- Day 1	1.35 ± 0.17	1.34 ± 0.19	p > 0.05
Group I- Day 3	2.01 ± 0.32	1.89 ± 0.27	p > 0.05
Group I- Day 7	3.83 ± 0.41	2.5 ± 0.55	p > 0.05
Group I- Day 14	5 ± 0.89	2.5 ± 0.57	p < 0.001**
Group I- Day 21	6.33 ± 0.81	3 ± 0.89	p < 0.001**
Group II- Day 1	1.41 ± 0.23	1.36 ± 0.21	p > 0.05
Group II- Day 3	2.13 ± 0.29	1.74 ± 0.27	p > 0.05
Group II- Day 7	3.17 ± 0.75	2.33 ± 0.52	p > 0.05
Group II- Day 14	4.67 ± 1.21	2.83 ± 0.75	p < 0.05*
Group II- Day 21	5.17 ± 1.17	3.33 ± 0.81	p < 0.05*

Intragroup Comparison of OTM Amount (Mean ± SD) (mm)

	Group	Day 1	Day 3	Day 7	Day 14	Day 21
Experimental Side	HMWHA	0.046 ± 0.009	0.47 ± 0.024	0.67 ± 0.024	0.989 ± 0.045	1.143 ± 0.054
	LMWHA	0.033 ± 0.008	0.45 ± 0.039	0.637 ± 0.039	0.964 ± 0.067	0.977 ± 0.035
	p	0.009*	0.165	0.07	0.233	<0.001**
Control Side	HMWHA	0.026 ± 0.01	0.341 ± 0.028	0.456 ± 0.028	0.54 ± 0.047	0.93 ± 0.048
	LMWHA	0.026 ± 0.007	0.346 ± 0.026	0.444 ± 0.026	0.552 ± 0.056	0.866 ± 0.037
	p	0.493	0.374	0.133	0.347	0.07

DOI: https://doi.org/10.2478/aoj-2024-0032 | Journal eISSN: 2207-7480 | Journal ISSN: 2207-7472

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Language: English

Page range: 172 - 183

Submitted on: Jul 1, 2024

Accepted on: Nov 1, 2024

Published on: Feb 5, 2025

Published by: Australian Society of Orthodontists Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 times per year

Related subjects:

Medicine,

Basic medical science,

Basic medical science, other

© 2025 Dler Mourad, Merve Goymen, Fatma Deniz Uzuner, Tuna Onal, Mehmet Ibrahim Tuglu, published by Australian Society of Orthodontists Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous article Volume 40 (2024): Issue 2 (July 2024)

Injectable hyaluronic acid: a method for accelerating alveolar bone remodelling during orthodontic tooth movement

Figures & Tables

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

BV/TV Percentage in Experimental and Control Groups (Mean ± SD) (%)

RANKL/OPG Ratio in Study Group (Mean ± SD)

Comparison of tooth movements between experimental and control sides on each observational day (Mean ± SD) (mm)

Number of Osteoclasts in Study Groups (Mean ± SD) (+1/+5 Score)

Intragroup Comparison of OTM Amount (Mean ± SD) (mm)

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