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Unbalanced cartilage calcification during development contributes to the formation of irregular articular surfaces as revealed by micro-CT images Cover

Unbalanced cartilage calcification during development contributes to the formation of irregular articular surfaces as revealed by micro-CT images

Australasian Orthodontic Journal
Volume 39 (2023): Issue 2 (July 2023)
By: Yuejiao Zhang,  Jing Zhang,  Lingfeng Xu and  Meiqing Wang  
Open Access
|Aug 2023

Figures & Tables

Figure 1.

Representative frontal and lateral views, and a sketch map of the incisors’ occlusal relationship in experimental group rats. BAE, bilateral anterior elevation; BAC, bilateral anterior crossbite; CON, blank control; GIDO, gradually induced disordered occlusion; MDO, mandibular deviated occlusion.

Figure 2.

The timeline of the entire experiment.

Figure 3.

Morphology of the lacunae revealed by gross observation, micro-CT images, and toluidine blue (TB) and von Kossa (VK) staining. For Gross morphology, micro-CT images, TB and VK staining images, bars = 500 μm; for HE staining images, bars = 100 μm.

Figure 4.

Representative histochemistry, immune-histochemistry (IHC) staining of the condylar lacuna induced by occlusal disturbance. Lacunae were detected by SO staining and IHC staining for Col-II, Col-X, OSX. For SO and IHC staining, bars = 100 μm.

Figure 5.

Representative sections with condylar lacunae induced by occlusal disturbance, stained with Tartrate-resistant acid phosphatase (TRAP) and Safranin O (SO), immune-histochemistry (IHC) for FN and immunofluorescence (IF) for cleaved caspase-3 (CCP3) and CD90. The black frames in the SO staining images showed the selected lacunae regions for IF staining. For SO, TRAP and IHC staining, bars = 100 μm. For IF staining, bars = 100 μm.

Figure 6.

The diagrammatic sketch for normal growth of the condyle (A) and lacunae formation (B).
DOI: https://doi.org/10.2478/aoj-2023-0025 | Journal eISSN: 2207-7480 | Journal ISSN: 2207-7472
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Language: English
Page range: 40 - 48
Submitted on: Feb 1, 2023
|
Accepted on: Jul 1, 2023
|
Published on: Aug 21, 2023
Published by: Australian Society of Orthodontists Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2023 Yuejiao Zhang, Jing Zhang, Lingfeng Xu, Meiqing Wang, published by Australian Society of Orthodontists Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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