Syndecan-1 expression during postnatal tooth and oral mucosa development in rats aged from two days to six weeks

De Angelis, Daniel D.; Sampson, Wayne J.; Wiebkin, Ole W.; Wilson, David F.

doi:10.2478/aoj-2002-0001

Abstract

Syndecans are a family of heparan sulphate proteoglycans that regulate cell-matrix interactions that influence cell growth, proliferation and morphology. The aim of this study was to observe changes in the expression of Syndecan-1 in the developing epithelium of the rat oral mucosa and in the epithelial cell rests of Malassez in the developing periodontium of normal rat molars, from late crown development through to early eruption. Immuno-histo-chemistry (Syndecan-1 N–18) and histochemistry (Alcec Blue) were used to observe changes in the expression of Syndecan-1 in rats aged two to 42 days. Results indicated that during normal tooth development in the rat, labelling or staining of variable intensity for Syndecan-1 was demonstrated in the stratified oral epithelium above the stratum basale in the rat tongue and palate, and in ameloblasts of the developing molar in rats aged two to 14 days. Histochemical staining of the predentine and dentine layers was consistent in all specimens. Labelling or staining for Syndecan-1 was negative in the rat periodontal ligament, which may suggest that either Syndecan-1 was not expressed during normal molar root development or that continued work is required for identification of a suitable label in rats.

References

Carey DJ. Syndecans: multifunctional cell surface coreceptors. Biochem J 1997; 327: 1–16.
Search in Google Scholar Back to article
Bernfield M, Kokenyesi R, Kato M, Hinkes MT, Spring J, Gallo, RL, Lose EJ. Biology of the Syndecans: a family of transmembrane heparan sulphate proteoglycans. Annu Rev Cell Biol 1992; 8: 365–93.
Search in Google Scholar Back to article
Salmivirta M, Elenius K, Vainio S, Hofer U, Chiquet-Ehrismann R, Thesleff I, Jalkanen M. Syndecan from embryonic tooth mesenchyme binds tenascin. J Biol Chem 1991; 266: 7733–9.
Search in Google Scholar Back to article
Thesleff I, Vaahtokari A, Vainio S, Jowett A. Molecular mechanisms of cell and tissue interactions during early tooth development. Anat Rec 1996; 245: 151–61.
Search in Google Scholar Back to article
Thesleff I, Vaahtokari A, Kettunen P, Aberg T. Epithelial-mesenchymal signalling during tooth development. Connect Tissue Res 1995; 32: 9–15.
Search in Google Scholar Back to article
Vainio S, Jalkanen M, Thesleff I. Syndecan and tenascin expression is induced by epithelial-mesenchymal interactions in embryonic tooth mesenchyme. J Cell Biol 1989; 108: 1945–54.
Search in Google Scholar Back to article
Thesleff I, Partanen Am, Vainio S. Epithelial-mesenchymal interactions in tooth morphogenesis: the roles of extracellular matrix growth factors, and cell surface receptors. J Craniofac Genet Dev Biol 1991; 11: 229–37.
Search in Google Scholar Back to article
Wiebkin, WO. Mucosubstances and structurally related macromolecular carbohydrates: procedures for specific substances. In: Woods AE, Ellis RC. Laboratory histopathology: a complete reference. Edinburgh: Churchill Livingstone, 1996; 1–43.
Search in Google Scholar Back to article
Scott JE, Stockwell RA. On the use and abuse of the critical electrolyte concentration approach to the localization of tissue polyanions. J Histochem Cytochem 1967; 15: 111–13.
Search in Google Scholar Back to article
Andreasen JO. Experimental dental traumatology: development of a model for external root resorption. Endod Dent Traumatol 1987; 3: 269–87.
Search in Google Scholar Back to article
Andersson, L, Jonsson, BG, Hammarstrom L, Blomlof L, Andreasen JO, Lindskog S. Evaluation of statistics and desirable experimental design of a histomorphometrical method for studies of root resorption. Endod Dent.Traumatol 1987; 3: 288–95.
Search in Google Scholar Back to article
Anttonen A, Kajanti M, Heikkila P, Jalkanen M, Joensuu H. Syndecan-1 expression has prognostic significance in head and neck carcinoma. Br J Cancer 1999; 79: 558–64.
Search in Google Scholar Back to article
Ten Cate AR. Oral histology: development, structure and function. St Louis: Mosby Year Book Inc. 1994.
Search in Google Scholar Back to article
Bartold PM, Narayanan AS. Biology of the periodontal connective tissues. Illinois: Quintessence, 1998.
Search in Google Scholar Back to article
Sawada T. Expression of basement membrane components in the dental papilla mesenchyme of monkey tooth germs: an immunochemical study. Connect Tissue Res 1995; 32: 55–61.
Search in Google Scholar Back to article
Gallagher JT, Lyon M, Steward WP. Structure and function of heparan sulphate proteoglycans. Biochem J 1986; 236: 313–25.
Search in Google Scholar Back to article
Hayashi K, Hayashi M, Jalkanen J, Firestone J, Trelstad R, Bernfield M. Immunocytochemistry of cell surface heparan sulphate proteoglycans in mouse tissues: a light and electron microscopic study. J Histochem Cytochem 1987; 35: 1079–88.
Search in Google Scholar Back to article
Jalkanen M, Rapraeger A, Bernfield M. Mouse mammary epithelial cells produce basement membrane and cell surface heparan sulphate proteoglycans containing distinct core proteins. J Cell Biol 1988; 106: 953–62.
Search in Google Scholar Back to article
Jalkanen M, Rapraeger A, Saunders S, Bernfield M. Cell surface proteoglycan of mouse mammary epithelial cells is shed by cleavage of its matrix-binding ectodomain from its membrane-associated domain. J Cell Biol 1987; 105: 3087–96.
Search in Google Scholar Back to article
Kirkham J, Brookes RC, Shore WA, Bonass WA, Robinson C. The effect of glycosaminoglycans on the mineralization of sheep periodontal ligament in vitro. Connect Tissue Res 1995; 33: 23–9 [345–51].
Search in Google Scholar Back to article
Friedl A, Chang Z, Tierney A, Rapraeger AC. Differential binding of fibroblastic growth factor-2 and -7 to basement membrane heparan sulphate. Am J Pathol 1997; 150: 1443–55.
Search in Google Scholar Back to article

Syndecan-1 expression during postnatal tooth and oral mucosa development in rats aged from two days to six weeks

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