RME-Induced Root Resorption and Repair: A Computerised 3-D Reconstruction

Kittel, P. W.; Sampson, WJ.

doi:10.2478/aoj-1994-0017

Abstract

This study was designed to evaluate the 3-D relation of epithelial rests of Malassez (ERM) to orthodontically induced resorption lacunae in human tooth roots, First premolars were extracted following rapid maxillary expansion, then they were prepared for transmission electron microscopy and sectioned. Serial 1 pm sections were used for the 3-D reconstructions and ultra thin sections were utilized to verify the identification of epithelial cells and blood vessel types. Each serial section was photographed and profiles of the resorption bays, epithelial cells and blood vessels were digitized and computer reconstructions performed with an IBM-based software programme.

The reconstructions supported the view that epithelial cell and blood vessel relation to the resorption lacunae appeared to be related to the level of activity of the bay. Possible functional roles for epithelial cells in the resorptive process are discussed.

References

Linge L, Linge BO. Patient characteristics and treatment variables associated with apical root resorption during orthodontic treatment. Am J Orthod 1991; 99: 35-43.
Search in Google Scholar Back to article
Reitan K. Behaviour of Malassez epithelial rests during orthodontic tooth movement. Acta Odont Scand 1961; 19: 443-68.
Search in Google Scholar Back to article
Reitan K. In: Graber TM and Swain BF, ed. Orthodontics current principles and techniques. St Louis: CVMosby, 1985: 101-93.
Search in Google Scholar Back to article
Andreasen JD. Review of root resorption systems and models. Etiology of root resorption and the homeostatic mechanisms of the periodontal ligament. In: The biological mechanisms of tooth eruption and root resorption. Ed. Z. Davidovitch, EBSCO Media, Birmingham AL 35233, 1988, pp9-21.
Search in Google Scholar Back to article
Brice GL, Sampson WJ, Sims MR. An ultrastructural evaluation of the relationship between epithelial rests of Malassez and orthodontic root resorption and repair in man. Aust Orthod J 1991; 12(2): 90-94.
Search in Google Scholar Back to article
Løe H and Waerhaug J. Experimental replantation of teeth in dogs and monkeys. Archs. Oral Biol 1961; 3: 176-184.
Search in Google Scholar Back to article
Spouge JD. A new look at the rests of Malassez. A review of their embryological origin, anatomy and possible role in periodontal health and disease. J Periodont 1980; 51: 437-444.
Search in Google Scholar Back to article
Pierce AM, Lindskog S and Hammarstrom L. Osteoclasts: structure and function. Electron Microsc. Rev. 1991; 4:1-45.
Search in Google Scholar Back to article
Harry MR, Sims MR. Root resorption in bicuspid intrusion. A scanning electron microscope study. Angle Orthod 1982; 52(3): 235-258.
Search in Google Scholar Back to article
Barber AF and Sims MR. Rapid maxillary expansion and external root resorption in man: a scanning electron microscope study. Am J Orthod 1981; 79(6): 630-652.
Search in Google Scholar Back to article
Rygh P. Orthodontic root resorption studies by electron microscope. Angle Orthod 1977; 47(1): 1-16.
Search in Google Scholar Back to article
Cookson MJ, Dykes E, Holman JG and Gray A. A microcomputer based system for generating realistic three dimensional shaded images reconstructed from serial sections. Eur J of Cell Biology Supp 1989; 25 (48): 69-72 (Abstract).
Search in Google Scholar Back to article
Rhodin JAG. The ultrastructure of mammalian arterioles and precapillary sphincters. J Ultrastruct Res 1967; 18: 181-223.
Search in Google Scholar Back to article
Rhodin JAG. Ultrastructure of mammalian venous capillaries, venules and small collecting veins. J Ultrastruct Res 1968; 25: 452-500.
Search in Google Scholar Back to article
Reeve M. Epithelial rests in the periodontal ligament of humans. J Dent Res I960; 39: 746.
Search in Google Scholar Back to article
Reeve CM and Wentz FM. The prevalence, morphology and distribution of epithelial rests in the human periodontal ligament. Oral Surg 1962; 15: 785-793.
Search in Google Scholar Back to article
Baba N, Baba M, Imamura M, Koga M, Ohsumi Y, Osumi M and Kanaya K. Serial section reconstruction using a computer graphics system: applications to intracellular structures in yeast cells and to the periodontal structure of dog’s teeth. Journal of Electron Microscopy Techniques 1989; 11: 16-26.
Search in Google Scholar Back to article
Huijsmans DP. Integration of software for computer-aided three dimensional reconstruction from parallel serial sections; a plea for standards. Eur J of Cell Biol 1989; Supp 25 (48): 57-59.
Search in Google Scholar Back to article
Suzuki M, Takahashi T and Ohuchi K. Three dimensional reconstruction of semi-gross biostructures and using ‘macroserials’ - 1 mm thick serial organ slices. J Microsc 1988; 149: 175-83.
Search in Google Scholar Back to article
Fawcett DW. In: The Cell. 2nd ed. Philadelphia, W.B. Saunders, 1981: 131.
Search in Google Scholar Back to article
Grupe HE, Ten Cate AR and Zander HA. A histochemical and radiobiological study of in vitro and in vivo human epithelial cell rest proliferation. Archs Oral Biol 1967; 12: 1321-1329.
Search in Google Scholar Back to article
Ten Cate AR. The epithelial cell rests of Malassez and the genesis of the dental cyst. Oral Surg 1972; 34: 956-964.
Search in Google Scholar Back to article
Kahn AJ and Malone JD. Lineage of bone resorbing cells: status and prospects for future research. In: The Biological Mechanisms of Tooth Eruption and Root Resorption. Ed. Z. Davidovitch, EBSCO Media, Birmingham AL 35233, 1988, pp 313-319.
Search in Google Scholar Back to article
Pettigrew DW, Sodek J, Wang HM and Brunette DM. Inhibitors of collagenolytic enzymes synthesized by fibroblasts and epithelial cells from porcine and macaque periodontal ligament. Archs Oral Biol 1980; 25: 269-274.
Search in Google Scholar Back to article
Lindskog S and Hammarstrom L. Evidence in favour of an anti-invasion factor in cementum or periodontal membrane of human teeth. Scand J Dent Res 1980; 88: 161-163.
Search in Google Scholar Back to article
Orban B. The epithelial network in the periodontal membrane. J Am Dent Assoc 1952; 44: 632-635.
Search in Google Scholar Back to article
Gurling FG. A transmission electron microscopic study of mouse molar cementogenesis. M.D.S. Thesis, The University of Adelaide, 1982.
Search in Google Scholar Back to article
Gurling FG and Sampson WJ. Epithelial root sheath changes during molar formation in the mouse. Archs Oral Biol 1985; 30: 757-764.
Search in Google Scholar Back to article
Fuchs E. Epidermal differentiation: the bare essentials. J Cell Biol 1990; 111 (6, pt 2): 2807-2814.
Search in Google Scholar Back to article
Leedham MD. The relationship between the epithelial cell rests of Malassez and experimental root resorption and repair in Macaca fascicularis. Honours Thesis, The University of Adelaide 1990,174pp.
Search in Google Scholar Back to article
Lindskog S, Blomlof L and Hammarstrom L. Evidence for a role of odontogenic epithelium in maintaining the periodontal space. J Clin Perio 1988;15:371-373.
Search in Google Scholar Back to article
Birek C, Heersche JNM, Jez D and Brunette DM. Secretion of a bone resorbing factor by epithelial cells cultured from porcine rests of Malassez. J Periodont Res 1983; 18: 75-81.
Search in Google Scholar Back to article
Brunette DM, Limeback H, Pettigrew D, Melcher AH and Sodek J. Proteins secreted in vitro by epithelial cells cultured from porcine periodontal ligament. J Dent Res 1979; 58: 244 (abstract).
Search in Google Scholar Back to article
Harris M and Goldhaber P. The production of bone resorbing factor by dental cysts in vitro. Br J Oral Surg 1973; 10: 334-338.
Search in Google Scholar Back to article
Brunette DM, Kanoza RJ, Marmary Y, Chan J and Melcher AH. Interactions between epithelial and fibroblast-like cells in culture derived from monkey periodontal ligament. J Cell Sci 1977; 27:127-140.
Search in Google Scholar Back to article
Brunette DM, Heersche JNM, Purdon AD, Sodek J, Moe HK and Assuras JN. In vitro cultural parameters and protein and prostaglandin secretion of epithelial cells derived from porcine rests of Malassez. Arch Oral Biol 1979; 24: 199-203.
Search in Google Scholar Back to article
Gao Z, Mackenzie IC, Williams DM, Crutchley AT, Leigh I and Lane EB. Patterns of keratin expression in rests of Malassez and periapical lesions. J Oral Pathol 1988; 17: 178-185.
Search in Google Scholar Back to article
Roth J. Post-embedding cytochemistry with gold-labelled reagents: a review. J Microsc 1986; 142:123.
Search in Google Scholar Back to article
Massoth DL and Dale BA. Immunohistochemical study of structural proteins in developing junctional epithelium. J Periodontal 1986; 57 (12): 756-763.
Search in Google Scholar Back to article
Juhl M, Reibel J and Stoltze K. Immunohistochemical distribution of keratin proteins in clinically healthy human gingival epithelia. Scand J Dent Res 1989; 97: 159-170.
Search in Google Scholar Back to article

RME-Induced Root Resorption and Repair: A Computerised 3-D Reconstruction

Abstract

Paradigm

My account