
Perspectives of Using Animal Tissues in the Treatment of Human Diseases
References
- AJT XenoBulletin Team (2025). Invited Perspective January 2025. Kicking off 2025: A spotlight on international clinical trial discussions, this month’s feature. Am. J. Transplant.
https://www.amjtransplant.org/xenobulletin-invited-insights-january-2025 - Ali A., Kurome M., Kessler B., Kemter E., Wolf E. (2024). What genetic modifications of source pigs are essential and sufficient for cell, tissue, and organ xenotransplantation? Transpl. Int., 37: 13681. doi: 10.3389/ti.2024.13681.
- Anand R.P., Layer J.V., Heja D., Hirose T., Lassiter G., Firl D.J., Paragas V.B., Akkad A., Chhangawala S., Colvin R.B., Ernst R.J., Esch N., Getchell K., Griffin A.K., Guo X., Hall K.C., Hamilton P., Kalekar L.A., Kan Y., Karadagi A., Li F., Low S.C., Matheson R., Nehring C., Otsuka R., Pandelakis M., Policastro R.A., Pols R., Queiroz L., Rosales I..A., Serkin W.T., Stiede K., Tomosugi T., Xue Y., Zentner G.E., Angeles-Albores D., Chris Chao J., Crabtree J.N., Harken S., Hinkle N., Lemos T., Li M., Pantano L., Stevens D., Subedar O.D., Tan X., Yin S., Anwar I.J., Aufhauser D., Capuano S., Kaufman D.B., Knechtle S.J., Kwun J., Shanmuganayagam D., Markmann J.F., Church G.M., Curtis M., Kawai T., Youd M.E., Qin W. (2023). Design and testing of a humanized porcine donor for xenotransplantation. Nature, 622: 393–401. doi: 10.1038/s41586-023-06594-4.
- Andriichuk B. (2024). Xenotransplantation in the European Union: towards harmonisation and accessibility. Humanit. Soc. Sci. Commun., 11: 1595
https://doi.org/10.1057/s41599-024-04128-5 . - Ata B., Montgomery R.A., Ozyoruk Y.N., Parent B., Schold J.D. (2025). Patient selection for xenotransplant human clinical trials: a data-driven approach. Transplantation. 109(9): 1471–1474. doi: 10.1097/TP.0000000000005384.
- Bailey L.L., Nehlsen-Cannarella S.L., Concepcion W., Jolley W.B. (1985). Baboon-to-human cardiac xenotransplantation in a neonate. JAMA, 254: 3321–3329.
- Bektas C.K., Luo J., Conley B., Le K.N., Lee K.B. (2025). 3D bioprinting approaches for enhancing stem cell-based neural tissue regeneration. Acta Biomater., 193: 20–48. doi: 10.1016/j.actbio.2025.01.006.
- Beyer M.C. (2025 a) First gene-edited porcine liver xenotransplant into human. Artif. Organs, 49: 923–924. doi: 10.1111/aor.15009.
- Beyer M.C. (2025 b). Third genetically modified kidney xenotransplantation in living human recipient. Artif. Organs, 49(3): 337–338. doi: 10.1111/aor.14945.
- Cabral M., Cheng K., Zhu D. (2024). Three-dimensional bioprinting of organoids: past, present, and prospective. Tissue Eng. Part A, 30: 314–321. doi: 10.1089/ten.TEA.2023.0209.
- Camenzind S. (2024). Xenotransplantation in the age of genome editing: results from the expert report for the federal ethics committee on nonhuman biotechnology with a special focus on animal ethics. Xenotransplantation, 31: e70008. doi: 10.1111/xen.70008.
- China Global TV Network (2024).
https://news.cgtn.com/news/2024-05-24/China-achieves-world-s-first-gene-edited-pig-liver-xenotransplantation-1tRTzGOSTjq/p.html Accessed 24.01.2025 - Cieślik K., Smorąg Z., Słomski R., Nowak M., Kawecki M. (2022). Biotechnologiczny opatrunek do leczenia ran oparzeniowych, sposób wykonania opatrunku biotechnologicznego do leczenia ran oparzeniowych i sposób zastosowania opatrunku biotechnologicznego do leczenia ran oparzeniowych. Biotechnology dressing for the treatment of burn wounds, how to make a biotechnology dressing for the treatment of burn wounds and how to use a biotechnology dressing for the treatment of burn wounds (Patent polski nr PL 240144). Urząd Patentowy Rzeczypospolitej Polskiej.
https://ewyszukiwarka.pue.uprp.gov.pl/search/pwp-details/P.404878 - Cohen J. (2025). The organ farm. Science, 388: 906–912. doi: 10.1126/science.adz2599.
- Cooper D.K.C., Cozzi E. (2024). Clinical pig heart xenotransplantation – where do we go from here? Transpl. Int., 37: 12592. doi: 10.3389/ti.2024.12592.
- Cooper D.K.C., Riella L.V., Kawai T., Fishman J.A., Williams W.W., Elias N., Madsen J.C., Pierson R.N. (2024). The time has come: The case for initiating pilot clinical trials of pig kidney xenotransplantation. Ann. Surg., 281: 204–209. doi: 10.1097/SLA.0000000000006529.
- Denner J. (2024). Porcine endogenous retroviruses in xenotransplantation. Nephrol. Dial. Transplant., 39: 1221–1227. doi: 10.1093/ndt/gfae023.
- Eisenson D., Hisadome Y., Santillan M., Iwase H., Chen W., Shimizu A., Schulick A., Gu D., Akbar A., Zhou A., Koenig K., Kuravi K., Rahman F., Sorrells L., Burdorf L., DeSmet K., Warren D., Peterson L., Lorber M., Ayares D., Cameron A., Yamada K. (2024). Consistent survival in consecutive cases of life-supporting porcine kidney xenotransplantation using 10GE source pigs. Nat. Commun., 15: 3361. doi: 10.1038/s41467-024-47679-6.
- Eurotransplant (2025).
https://www.eurotransplant.org/ . Accession 28.01.2025. - Fatima H., Eltoum I.E., Kumar V., Anderson D.J., Locke J.E. (2025). Immunofluorescence and electron microscopy in genetically engineered pig-to-human kidney xenotransplantation: A case report. Am. J. Kidney Dis., 86: 563–569. doi: 10.1053/j.ajkd.2025.04.021.
- Fieldhouse R. (2025 a). ‘Amazing feat’: US man still alive six months after pig kidney transplant. Nature, 645: 571–572. doi: 10.1038/d41586-025-02851-w.
- Fieldhouse R. (2025 b). Pig lung transplanted into a person in world first. Nature, 645(8079): 20. doi: 10.1038/d41586-025-02708-2.
- Frankowski J., Kurzątkowska M., Sobczak M., Piotrowska U. (2023). Utilization of 3D bioprinting technology in creating human tissue and organoid models for preclinical drug research – State-of-the-art. Int. J. Pharm., 644: 123313. doi: 10.1016/j.ijpharm.2023.123313.
- Gabriel M., Pukacki F., Dzieciuchowicz Ł., Oszkinis G., Checiński P. (2004). Cryopreserved arterial allografts in the treatment of prosthetic graft infections. Eur. J. Vasc. Endovasc. Surg., 27: 590–596.
- Gadre M., Kasturi M., Agarwal P., Vasanthan K.S. (2024). Decellularization and their significance for tissue regeneration in the era of 3D bioprinting. ACS Omega, 9: 7375–7392. doi: 10.1021/acsomega.3c08930.
- Gałka S., Nowak E., Bednarek I. (2022). Selective human cells in vitro transduction with porcine endogenous retrovirus (PERV). Acta Virol., 66: 110–126. doi: 10.4149/av_2022_201.
- Galli C. (2025). Current techniques of gene editing in pigs for xenotransplantation. Transpl. Int., 38: 13807. doi: 10.3389/ti.2025.13807.
- Gierek M., Klama-Baryła A., Łabuś W., Bergler-Czop B., Pietrauszka K., Niemiec P. (2023). Platelet-rich plasma and acellular dermal matrix in the surgical treatment of hidradenitis suppurativa: a comparative retrospective study. J. Clin. Med., 12: 2112. doi: 10.3390/jcm12062112.
- Goerlich C.E., Griffith B., Hanna P., Hong S.N., Ayares D., Singh A.K., Mohiuddin M.M. (2023). The growth of xenotransplanted hearts can be reduced with growth hormone receptor knockout pig donors. J. Thorac. Cardiovasc. Surg., 165: e69–e81. doi: 10.1016/j.jtcvs.2021.07.051.
- Griffith B.P., Goerlich C.E., Singh A.K., Rothblatt M., Lau C.L., Shah A., Lorber M., Grazioli A., Saharia K.K., Hong S.N., Joseph S.M., Ayares D., Mohiuddin M.M. (2022). Genetically modified porcine-to-human cardiac xenotransplantation. N. Engl. J. Med., 387: 35–44. doi: 10.1056/NEJMoa2201422.
- Griffith B.P., Grazioli A., Singh A.K., Tully A., Galindo J., Saharia K.K., Shah A., Strauss E.R., Odonkor P.N., Williams B., Silverman H.J., Burke A., Drachenberg C.B., Wells C.L., Dickfeld T., Hong S.N., Hicks A.J. 3rd, Ananthram M., Gupta A., Christenson R.H., Tamburro L., Zhang T., Hershfeld A., Lewis B., Feller E.D., Kuravi K., Sorrells L., Morgand E., Mezine F., Goutaudier V., Rothblatt M., Lau C.L., Taylor B., Perrin S., Loupy A., Ayares D., Mohiuddin M.M. (2025). Transplantation of a genetically modified porcine heart into a live human. Nat. Med., 31: 589–598. doi: 10.1038/s41591-024-03429-1.
- Grześkowiak B.F., Hryhorowicz M., Tuśnio K., Grzeszkowiak M., Załęski K., Lipiński D., Zeyland J., Mykhaylyk O., Słomski R., Jurga S., Woźniak A. (2016). Generation of transgenic porcine fibroblast cell lines using nanomagnetic gene delivery vectors. Mol. Biotechnol., 58: 351–361. doi: 10.1007/s12033-016-9934-1.
- Hara H., Wang Y. (2025). Beyond transplantation: Gene-edited pig liver supports function in a human host. Cell Stem Cell, 32: 1034–1035. doi: 10.1016/j.stem.2025.06.005.
- Hawthorne W.J., Pierson R.N. 3rd, Buhler L., Cowan P.J., Fishman J., Bottino R., Meier R.P.H., Brenner P., Wolf E., Cozzi E., Mohiuddin M.M., Council of the International Xenotransplantation Association (IXA) (2025). International Xenotransplantation Association (IXA) position paper on the history, current status, and regulation of xenotransplantation. Transplantation, 109: 1301–1312. doi: 10.1097/TP.0000000000005373.
- He J., Shi J., Yang C., Peng G., Ju C., Zhao Y., Liu H., He P., Liu X., Zhang Z., Chen C., Pan D., Yang Z., Guang W., Li H., Chen Z., Liu M., Liang H., Huang W., Jeon K., Chen-Yoshikawa T.F., Rucker A.J., Lal A., Zhong N., Zhang K., Liu X., Xu X. (2025). Pig-to-human lung xenotransplantation into a brain-dead recipient. Nat. Med., 31: 3388–3393. doi: 10.1038/s41591-025-03861-x.
- Health Resources and Services Administration (2025).
https://www.organdonor.gov/ . Accession 22.09.2025. - Ho B.X., Teo A.K.K., Ng N.H.J. (2024). Innovations in bio-engineering and cell-based approaches to address immunological challenges in islet transplantation. Front. Immunol., 15: 1375177. doi: 10.3389/fimmu.2024.1375177.
- Hryhorowicz M., Grześkowiak B., Mazurkiewicz N., Śledziński P., Lipiński D., Słomski R. (2019). Improved delivery of CRISPR/Cas9 system using magnetic nanoparticles into porcine fibroblast. Mol. Biotechnol., 61: 173–180. doi: 10.1007/s12033-018-0145-9.
- Huo X., Sun X., Xing X., Lu J., Zhang J., Jiang Y., Zhu X., Li C., Lv J., Guo M., Cao L., Liu X., Chen Z., Pan D., He S., Zhang C., Du X. (2025). Genome-wide genetic mutations accumulated in pigs genome-edited for xenotransplantation and their filial generation. Genomics Proteomics Bioinformatics, 23: qzaf071. doi: 10.1093/gpbjnl/qzaf071.
- Judd E., Kumar V., Porrett P.M., Hyndman K.A., Anderson D.J., Jones-Carr M.E., Shunk A., Epstein D.R., Fatima H., Katsurada A., Satou R., Navar L.G., Locke J.E. (2024). Physiologic homeostasis after pig-to-human kidney xenotransplantation. Kidney Int., 105: 971–979. doi: 10.1016/j.kint.2024.01.016.
- Juric I., Raynaud M., Skoric L., Al-Awadhi S., Truchot A., Sablik M., Ma X., Lv K., Zhang H., Louis K., Basic-Jukic N., Tissier R., Hauet T., Cozzi E., Oniscu G.C., Mangiola M., Tector J.A., Riella L.V., Locke J.E., Samuel D., Meier R.P.H., Mohiuddin M.M., Montgomery R.A., Loupy A. (2025). Mapping the evolution of solid organ xenotransplantation research: A systematic review. Xenotransplantation, 32: e70058. doi: 10.1111/xen.70058.
- Kaiser M., Gunther P., Forsberg E.M., Mollaki V., Rimstad E., Marusic A. (2024). Is Europe ready for xenotransplantation? Humanit. Soc. Sci. Commun., 11: 1582.
https://doi.org/10.1057/s41599-024-04126-7 . - Karolinska Institutet (2024). News from Karolinska Institutet. The Macchiarini case: Timeline, 29.01.2024,
https://news.ki.se/news-archive/the-macchiarini-case-timeline . Accession 22.01.2025 - Kawamura T., Saito S., Taguchi T., Yoshioka D., Kawamura A., Misumi Y., Yamauchi T., Miyagawa S., Miyagawa S. (2025). Current status and prospects of genetically modified porcine-to-human cardiac xenotransplantation. J. Artif. Organs, 28: 348–353. doi: 10.1007/s10047-025-01504-z.
- Kemter E., Schnieke A., Fischer K., Cowan P.J., Wolf E. (2020). Xeno-organ donor pigs with multiple genetic modifications – the more the better? Curr. Opin. Genet. Dev., 64: 60–65. doi: 10.1016/j.gde.2020.05.034.
- Kemter E., Citro A., Wolf-van Buerck L., Qiu Y., Böttcher A., Policardi M., Pellegrini S., Valla L., Alunni-Fabbroni M., Kobolák J., Kessler B., Kurome M., Zakhartchenko V., Dinnyes A., Cyran C.C., Lickert H., Piemonti L., Seissler J., Wolf E. (2022). Transgenic pigs expressing near infrared fluorescent protein – A novel tool for noninvasive imaging of islet xenotransplants. Xenotransplantation, 29: e12719. doi: 10.1111/xen.12719.
- Kimsa-Dudek M., Strzalka-Mrozik B., Kimsa M.W., Blecharz I., Gola J., Skowronek B., Janiszewski A., Lipinski D., Zeyland J., Szalata M., Slomski R., Mazurek U. (2015). Screening pigs for xenotransplantation: expression of porcine endogenous retroviruses in transgenic pig skin. Transgenic Res., 24: 529–536. doi: 10.1007/s11248-015-9871-y.
- Kitala D., Klama-Baryła A., Łabuś W., Kraut M., Glik J., Kawecki M., Joszko K., Gzik-Zroska B. (2020). Porcine transgenic, acellular material as an alternative for human skin. Transplant. Proc., 52: 2218–2222. doi: 10.1016/j.transproceed.2020.01.125.
- Klama-Baryła A., Kitala D., Łabuś W., Kraut M., Szapski M., Słomski R. (2020). Is transgenic porcine skin as good as allogeneic skin for regenerative medicine? Comparison of chosen properties of xeno- and allogeneic material. Transplant. Proc., 52: 2208–2217. doi: 10.1016/j.transproceed.2020.01.109.
- Kögel J., Ernst P., Sauermeister J., Marckmann G. (2024). Ethical implications of social science research on xenotransplantation. Xenotransplantation, 31: e70004. doi: 10.1111/xen.70004.
- Kögel J., Schmoeckel M., Marckmann G. (2025). Patient choice as inclusion criterion for first clinical trials of xenotransplantation. J. Med. Ethics, 51: 172–173. doi: 10.1136/jme-2024-110673.
- Lewis T. (2024). Woman becomes first person to receive both pig kidney transplant and heart pump. Scientific American.
https://www.scientificamerican.com/article/woman-receives-genetically-modified-pig-kidney-transplant-after-heart-pump/ . Accessed 24.01.2025 - Lipiński D., Nowak-Terpiłowska A., Hryhorowicz M., Jura J., Korcz A., Słomski R., Juzwa W., Mazurkiewicz N., Smorąg Z., Zeyland J. (2019). Production of ZFN-mediated GGTA1 knock-out pigs by microinjection of gene constructs into pronuclei of zygotes. Pol. J. Vet. Sci., 22: 91–100. doi: 10.24425/pjvs.2018.125611.
- Loupy A., Goutaudier V., Giarraputo A., Mezine F., Morgand E., Robin B., Khalil K., Mehta S., Keating B., Dandro A., Certain A., Tharaux P.L., Narula N., Tissier R., Giraud S., Hauet T., Pass H.I., Sannier A., Wu M., Griesemer A., Ayares D., Tatapudi V., Stern J., Lefaucheur C., Bruneval P., Mangiola M., Montgomery R.A. (2023). Immune response after pig-to-human kidney xenotransplantation: a multimodal phenotyping study. Lancet, 402: 1158–1169. doi: 10.1016/S0140-6736(23)01349-1.
- Ma Y., Deng B., He R., Huang P. (2024). Advancements of 3D bioprinting in regenerative medicine: Exploring cell sources for organ fabrication. Heliyon, 10: e24593. doi: 10.1016/j.heliyon.2024.e24593.
- Mallapaty S. (2024). First pig-to-human liver transplant recipient ‘doing very well’. Nature, 630: 18. doi: 10.1038/d41586-024-01613-4.
- Mallapaty S., Kozlov M. (2024). First pig kidney transplant in a person: what it means for the future. Nature, 628: 13–14. doi: 10.1038/d41586-024-00879-y.
- Matczyńska D., Sypniewski D., Gałka S., Sołtysik D., Loch T., Nowak E., Smorąg Z., Bednarek I. (2018). Analysis of swine leukocyte antigen class I gene profiles and porcine endogenous retrovirus viremia level in a transgenic porcine herd inbred for xenotransplantation research. J. Vet. Sci., 19: 384–392. doi: 10.4142/jvs.2018.19.3.384.
- Matsumura S., Kakuta Y., Maegawa-Higa Y., Fukae S., Tanaka R., Nakazawa S., Yamanaka K., Miyagawa S., Nonomura N. (2025). Differences between xenotransplantation and allogeneic kidney transplantation: the current situation and future challenges in Japan. J. Artif. Organs, 28: 336–342. doi: 10.1007/s10047-025-01506-x.
- Mazurek U., Kimsa M.C., Strzalka-Mrozik B., Kimsa M.W., Adamska J., Lipinski D., Zeyland J., Szalata M., Slomski R., Jura J., Smorag Z., Nowak R., Gola J. (2013). Quantitative analysis of porcine endogenous retroviruses in different organs of transgenic pigs generated for xenotransplantation. Curr. Microbiol., 67: 505–514. doi: 10.1007/s00284-013-0397-3.
- Meier R.P.H., Longchamp A., Mohiuddin M., Manuel O., Vrakas G., Maluf D.G., Buhler L.H., Muller Y.D., Pascual M. (2021). Recent progress and remaining hurdles toward clinical xenotransplantation. Xenotransplantation, 28: e12681. doi: 10.1111/xen.12681.
- Meier R.P.H., Pierson R.N. 3rd, Fishman J.A., Buhler L.H., Bottino R., Ladowski J.M., Ekser B., Wolf E., Brenner P., Ierino F., Mohiuddin M., Cooper D.K.C., Hawthorne W.J. (2025). International Xenotransplantation Association (IXA) position paper on kidney xenotransplantation. Transplantation, 109: 1313–1328. doi: 10.1097/TP.0000000000005372.
- Mohiuddin M.M. (2025). Asking the right questions: Larry Faucette’s journey through xenotransplant. Xenotransplantation, 32: e70014. doi: 10.1111/xen.70014.
- Montgomery R.A., Stern J.M., Lonze B.E., Tatapudi V.S., Mangiola M., Wu M., Weldon E., Lawson N., Deterville C., Dieter R.A., Sullivan B., Boulton G., Parent B., Piper G., Sommer P., Cawthon S., Duggan E., Ayares D., Dandro A., Fazio-Kroll A., Kokkinaki M., Burdorf L., Lorber M., Boeke J.D., Pass H., Keating B., Griesemer A., Ali N.M., Mehta S.A., Stewart Z.A. (2022). Results of two cases of pig-to-human kidney xenotransplantation. N. Engl. J. Med., 386: 1889–1898. doi: 10.1056/NEJMoa2120238.
- Montgomery R.A., Griesemer A.D., Segev D.L., Sommer P. (2024). The decedent model: A new paradigm for de-risking high stakes clinical trials like xenotransplantation. Am. J. Transplant., 24: 526–532. doi: 10.1016/j.ajt.2024.01.035.
- Nascimbene A., Bark D., Smadja D.M. (2024). Hemocompatibility and biophysical interface of left ventricular assist devices and total artificial hearts. Blood, 143: 661–672. doi: 10.1182/blood.2022018096.
- Nature Biotechnology (2024). First gene-edited pig kidney transplant. Nat. Biotechnol., 42: 543. doi: 10.1038/s41587-024-02223-1.
- Nature Biotechnology (2025). FDA says GM pigs safe to eat. Nat. Biotechnol., 43: 839. doi: 10.1038/s41587-025-02716-7.
- Paterlini M. (2023). Paolo Macchiarini: Disgraced surgeon is sentenced to 30 months in prison. BMJ, 381: 1442. doi: 10.1136/bmj.p1442.
- Peterson L., Yacoub M.H., Ayares D., Yamada K., Eisenson D., Griffith B.P., Mohiuddin M.M., Eyestone W., Venter J.C., Smolenski R.T., Rothblatt M. (2024). Physiological basis for xenotransplantation from genetically modified pigs to humans. Physiol. Rev., 104: 1409–1459. doi: 10.1152/physrev.00041.2023.
- Poltransplant (2025). Statystyka 2024 r.
https://poltransplant.org.pl/ . Accession 28.01.2025 - Pullen L.C. (2024). Xenotransplantation moves toward clinical trials. Am. J. Transplant., 24: 509–511. doi: 10.1016/j.ajt.2024.03.001.
- Rana D., Rangel V.R., Padmanaban P., Trikalitis V.D., Kandar A., Kim H.W., Rouwkema J. (2025). Bioprinting of aptamer-based programmable bioinks to modulate multi-scale microvascular morphogenesis in 4D. Adv. Healthc. Mater., 14: e2402302. doi: 10.1002/adhm.202402302.
- Reemtsma K., Mccracken B.H., Schlegel J.U., Pearl M.A., Pearce C.W., Dewitt C.W., Smith P.E., Hewitt R.L., Flinner R.L., Creech O. Jr. (1964). Renal heterotransplantation in man. Ann. Surg,. 160: 384–410. doi: 10.1097/00000658-196409000-00006.
- Regalado A. (2024). A brain-dead man was attached to a gene-edited pig liver for three days. MIT Technology Review.
www.technologyreview.com/2024/01/18/1086791/brain-dead-man-gene-edited-pig-liver/ - Reichart D., Puga Yung G., Wolf E. (2024). Multimodal deep phenotyping provides insights into early human anti-porcine xeno-organ responses. Transplantation, 108: 2157–2158. doi: 10.1097/TP.0000000000005137.
- Ryczek N., Hryhorowicz M., Lipiński D., Zeyland J., Słomski R. (2020). Evaluation of the CRISPR/Cas9 genetic constructs in efficient disruption of porcine genes for xenotransplantation purposes along with an assessment of the off-target mutation formation. Genes (Basel), 11: 713. doi: 10.3390/genes11060713.
- Samiec M., Skrzyszowska M. (2014). Biological transcomplementary activation as a novel and effective strategy applied to the generation of porcine somatic cell cloned embryos. Reprod. Biol., 14: 128–139. doi: 10.1016/j.repbio.2013.12.006.
- Samiec M., Wiater J., Wartalski K., Skrzyszowska M., Trzcińska M., Lipiński D., Jura J., Smorąg Z., Słomski R., Duda M. (2022). The relative abundances of human leukocyte antigen-E, α-galactosidase A and α-Gal antigenic determinants are biased by trichostatin A-dependent epigenetic transformation of triple-transgenic pig-derived dermal fibroblast cells. Int. J. Mol. Sci., 23: 10296. doi: 10.3390/ijms231810296.
- Sikora B., Skubis-Sikora A., Kimsa-Furdzik M., Ciszek W., Kostrzewski M., Stojko J., Mazurek U., Gola J. (2019). Adipose-derived stem cells undergo differentiation after co-culture with porcine limbal epithelial stem cells. Stem Cell Res., 41: 101609. doi: 10.1016/j.scr.2019.101609.
- Słomski R., Strauss E., Lipiński D., Smorąg Z., Jura J., Skrzyszowska M., Samiec M., Wilczek P., Klama-Baryła A. (2021). Innowacyjna technologia wykorzystania tkanek transgenicznych świń w ksenotransplantacji. In: Nauka dla Społeczeństwa. Osiągnięcia Instytutu Genetyki Człowieka PAN, Słomski R. (ed.) (in Polish). Wydawnictwo Instytutu Genetyki Człowieka PAN, Poznań, Poland, pp. 149–174.
- Smorąg Z., Słomski R., Cierpka L. (2006). Editors. Biotechnologiczne i medyczne podstawy ksenotransplantacji (in Polish). Wydawnictwo OWN Poznań, Poland, 388 pp.
- Smorąg Z., Słomski R., Modliński J.A. (2008). Editors. Od genomu tura po ksenotransplantacje (in Polish). Wydawnictwo OWN Poznań, Poland, 182 pp.
- Smorąg Z., Słomski R., Cierpka L. (2013). Editors. Biotechnologiczne i medyczne podstawy ksenotransplantacji (in Polish). Wydawnictwo OWN Poznań, Poland, 2nd ed. updated and expanded, 452 pp.
- Starzl T.E., Fung J., Tzakis A., Todo S., Demetris A.J., Marino I.R., Doyle H., Zeevi A., Warty V., Michaels M., Kusne S., Rudert W.A., Trucco M. (1993). Baboon-to-human liver transplantation. Lancet, 341: 65–71. doi: 10.1016/0140-6736(93)92553-6.
- Sucu S., Yankol Y., Fernandez L.A., Ekser B. (2025). Liver xenotransplantation: A path to clinical reality. Transpl. Int., 37: 14040. doi: 10.3389/ti.2024.14040.
- Sumbalova Koledova Z. (2024). 3D cell culture: Techniques for and beyond organoid applications. Methods Mol. Biol., 2764: 1–12. doi: 10.1007/978-1-0716-3674-9_1.
- Tao K.S., Ling Y.W., Zhang X., Yang Z.X., Zhang H.T., Yue S.Q., Yang Y.L., Song W.J., Wang D.S., Liu Z.C., Li H.M., Chen Y., Ding R., Li L., Li M., Lin Z.B., Xu H., Wei D., Zhang H., Duan J.L., Zhuang R., Long E.W., Zhou L., Pan D.K., Dou K.F., Wang L. (2025 a). Immune cell landscape in a human decedent receiving a pig liver xenograft. Nat. Med., 31: 2611–2621. doi: 10.1038/s41591-025-03860-y.
- Tao K.S., Yang Z.X., Zhang X., Zhang H.T., Yue S.Q., Yang Y.L., Song W.J., Wang D.S., Liu Z.C., Li H.M., Chen Y., Ding R., Sun S.R., Yu M., Li J.P., Duan W.X., Wang Z., Wang J.W., Liu J.Y., Zheng M.W., Zhang X.J., Yin W., Qin W.J., Bian D.M., Li L., Li M., Lin Z.B., Xu H., Wei D., Zhang H., Duan J.L., Pan D.K., Dong H.L., Wang L., Dou K.F. (2025 b). Gene-modified pig-to-human liver xenotransplantation. Nature, 641: 1029–1036. doi: 10.1038/s41586-025-08799-1.
- Trzcińska M., Bryła M., Bochenek M., Słomski R., Smorag Z. (2009). Assessment of plasma membrane and chromatin structure of sperm from transgenic and non-transgenic boars. Theriogenology, 72: 1141–1147. doi: 10.1016/j.theriogenology.2009.07.010.
- Veeravalli R.S., Vejandla B., Savani S., Nelluri A., Peddi N.C. (2023). Three-dimensional bioprinting in medicine: A comprehensive overview of current progress and challenges faced. Cureus, 15: e41624. doi: 10.7759/cureus.41624.
- Wang W., He W., Ruan Y., Geng Q. (2022). First pig-to-human heart transplantation. Innovation (Camb.), 3: 100223. doi: 10.1016/j.xinn.2022.100223.
- Wiater J., Samiec M., Skrzyszowska M., Lipiński D. (2021 a). Trichostatin A-assisted epigenomic modulation affects the expression profiles of not only recombinant human α1,2-fucosyltransferase and α-galactosidase A enzymes but also Galα1→3Gal epitopes in porcine bi-transgenic adult cutaneous fibroblast cells. Int. J. Mol. Sci., 22: 1386. doi: 10.3390/ijms22031386.
- Wiater J., Samiec M., Wartalski K., Smorąg Z., Jura J., Słomski R., Skrzyszowska M., Romek M. (2021 b). Characterization of mono- and bi-transgenic pig-derived epidermal keratinocytes expressing human FUT2 and GLA genes – in vitro studies. Int. J. Mol. Sci., 22: 9683. doi: 10.3390/ijms22189683.
- Wilczek P., Lesiak A., Niemiec-Cyganek A., Kubin B., Slomski R., Nozynski J., Wilczek G., Mzyk A., Gramatyka M. (2015). Biomechanical properties of hybrid heart valve prosthesis utilizing the pigs that do not express the galactose-α-1,3-galactose (α-Gal) antigen derived tissue and tissue engineering technique. J. Mater. Sci. Mater. Med., 26: 5329. doi: 10.1007/s10856-014-5329-7.
- Wolf E., Reichart B., Moretti A., Laugwitz K.L. (2023). Designer pigs for xenogeneic heart transplantation and beyond. Dis. Model Mech., 16: dmm050177. doi: 10.1242/dmm.050177.
- Xu K., Zhao H., Jia B., Wang J., Siddig N.A.M.A., Jamal M.A., Mao A., Liu K., Cheng W., Yang C., Wei T., Zhu F., Huo X., Jiao D., Guo J., Zhao H., Cheng W., Zhang Y., Zhang X., Jiang L., Zhang Z., Zhang W., Liang T., Zhao H.Y., Sun B-C., Wei H-J. (2025). Specific pathogen free ten gene-edited donor pigs for xenotransplantation. Protein Cell., 16: 1002–1016. doi: 10.1093/procel/pwaf075.
- Zeyland J., Gawrońska B., Juzwa W., Jura J., Nowak A., Słomski R., Smorąg Z., Szalata M., Woźniak A., Lipiński D. (2013). Transgenic pigs designed to express human α-galactosidase to avoid humoral xenograft rejection. J. Appl. Genet., 54: 293–303. doi: 10.1007/s13353-013-0156-y.
- Zeyland J., Woźniak A., Gawrońska B., Juzwa W., Jura J., Nowak A., Słomski R., Smorąg Z., Szalata M., Mazurek U., Lipiński D. (2014). Double transgenic pigs with combined expression of human α1,2-fucosyltransferase and α-galactosidase designed to avoid hyperacute xenograft rejection. Arch. Immunol. Ther. Exp. (Warsz.), 62: 411–422. doi: 10.1007/s00005-014-0280-3.
- Zhang H., Chang Y., Song J. (2024). Xenotransplantation: How close are we to clinical applications? Life Med., 3: lnae037. doi: 10.1093/lifemedi/lnae037.
- Zhao W. (2024). Pig organs in humans: a forum on xenotransplantation. Natl. Sci. Rev., 11: nwae208. doi: 10.1093/nsr/nwae208.
- Zhou Y., Zhou S., Wang Q., Zhang B. (2024). Mitigating cross-species viral infections in xenotransplantation: Progress, strategies, and clinical outlook. Cell Transplant., 33: 9636897241226849. doi: 10.1177/09636897241226849.
DOI: https://doi.org/10.2478/aoas-2026-0013 | Journal eISSN: 2300-8733 (formerly 1642-3402) | Journal ISSN: 1642-3402
Language: English
Submitted on: Oct 22, 2025
Accepted on: Jan 19, 2026
Published on: Oct 7, 2026
Published by: National Research Institute of Animal Production
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
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© 2026 Marlena Szalata, Karolina Wielgus, Mikołaj Danielewski, Milena Szalata, Andrzej Hnatyszyn, Joanna Bartkowiak-Wieczorek, Zdzisław Smorąg, Ryszard Słomski, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 4.0 License.