Pyometra does not affect some molecular quality-related parameters of canine oocytes

Pawlak, Piotr; Warzych, Ewelina; Madeja, Zofia E.; Nowak, Tomasz; Lechniak, Dorota

doi:10.2478/aoas-2020-0062

Abstract

Cystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is a commonly applied treatment. Thus the only way to take advantage of the genetic potential of valuable females is application of assisted reproductive techniques (ART) mainly in vitro embryo production (IVP) or in some exceptional cases animal cloning by somatic cell nuclear transfer (SCNT). The aim of our study was to examine a potential effect of the CEH/P status on the quality of oocytes from females subjected to OHE. In total, 828 immature oocytes collected from ovaries of 33 bitches (21 control, 12 CEH/P) were subjected to genetic analyses (mRNA expression of two maternal-effect genes: GDF-9, OCT4 and mitochondrial DNA (mtDNA) content). Oocytes of CEH/P females were characterized by a higher mtDNA content (471 696) than gametes of their healthy counterparts (368 175; P<0.005). Transcripts for the two genes were detected in all samples and the mRNA level was not affected by the CEH/P status. In conclusion, the CEH/P complex does not exert a negative effect on oocyte quality reflected by the two parameters examined in this study.

References

Apparicio M., Ferreira C.R., Tata T., Santos V.G., Alves A.E., Mostachio G.Q., Pires-Butler E.A., Motheo T.F., Padilha L.C., Pilau E.J., Gozzo F.C., Eberlin M.N., Lo Turco E.G., Luvoni G.C., Vicente W.R.R. (2012). Chemical composition of lipids present in cat and dog oocyte by matrix-assisted desorption ionization mass spectrometry (MALDI-MS). Reprod. Domest. Anim., 47: 113–117.
Search in Google Scholar Back to article
Apparicio M., Mostachio G.Q., Motheo T.F., Alves A.E., Padilha L., Pires-Butler E.A., Savi P.A., Uscategui R.A., Luvoni G.C., Vicente W.R. (2015). Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems. Reprod. Fert. Develop., 27: 1082–1087.
Search in Google Scholar Back to article
Bradley J., Swann K. (2019). Mitochondria and lipid metabolism in mammalian oocytes and early embryos. Int. J. Dev. Biol., 63: 93–103.
Search in Google Scholar Back to article
Chastant-Maillard S., Viarisde Lesegno C., Chebrout M., Thoumire S., Meylheuc T., Fontbonne A., Chodkiewicz M., Saint-Dizier M., Reynaud K. (2011). The canine oocyte: uncommon features of in vivo and in vitro maturation. Reprod. Fert. Develop., 23: 391–402.
Search in Google Scholar Back to article
De Avila Rodrigues B., Rodrigues J.L. (2003). Influence of reproductive status on in vitro oocyte maturation in dogs. Theriogenology, 60: 59–66.
Search in Google Scholar Back to article
Delos Reyes M., Rojas C., Hugo Parraguez V., Palomino J. (2013). Expression of growth differentiation factor 9 (GDF-9) during in vitro maturation in canine oocytes. Theriogenology, 80: 587–596.
Search in Google Scholar Back to article
Egenvall A., Hagman R., Bonnett B.N., Hedhammar A., Olson P., Lagerstedt A.S. (2001). Breed risk of pyometra in insured dogs in Sweden. J. Vet. Int. Med., 15: 530–538.
Search in Google Scholar Back to article
El Shourbagy S.H., Spikings E.C., Freitas M., St John J.C. (2006). Mitochondria directly influence fertilisation outcome in the pig. Reproduction, 131: 233–245.
Search in Google Scholar Back to article
Hagman R. (2017). Canine pyometra: What is new? Reprod. Domest. Anim., 52, Suppl 2: 288–292.
Search in Google Scholar Back to article
Hishinuma M., Minami S., Okamoto Y., Miyatake K., Sekine J. (2004). Recovery, morphological quality, and in vitro maturation of follicular oocytes from bitches with pyometra. Theriogenology, 62: 1652–1662.
Search in Google Scholar Back to article
Jang G., Lee B. (2015). Update on the first cloned dog and outlook for canine cloning. Cell. Reprogram., 17: 325–326.
Search in Google Scholar Back to article
Kim M.J., Oh H.J., Kim G.A., Setyawan E.M.N., Choi Y.B., Lee S.H., Petersen-Jones S.M., Ko C.J., Lee B.C. (2017). Birth of clones of the world’s first cloned dog. Sci. Rep., 7: 15235.
Search in Google Scholar Back to article
Li L., Zheng P., Dean J. (2010). Maternal control of early mouse development. Development, 137: 859–870.
Search in Google Scholar Back to article
Lonergan P., Fair T. (2016). Maturation of oocytes in vitro. Annu, Rev. Anim. Biosci., 4: 255–268.
Search in Google Scholar Back to article
May-Panloup P., Chretien M.F., Jacques C., Vasseur C., Malthiery Y., Reynier P. (2005). Low oocyte mitochondrial DNA content in ovarian insufficiency. Hum. Reprod., 20: 593–597.
Search in Google Scholar Back to article
May-Panloup P., Chretien M.F., Malthiery Y., Reynier P. (2007). Mitochondrial DNA in the oocyte and the developing embryo. Curr. Top. Dev. Biol., 77: 51–83.
Search in Google Scholar Back to article
Melandri M., Veronesi M.C., Pisu M.C., Majolino G., Alonge S. (2019). Fertility outcome after medically treated pyometra in dogs. J. Vet. Sci., 20: e39.
Search in Google Scholar Back to article
Moussa M., Shu J., Zhang X.H., Zeng F. (2015). Maternal control of oocyte quality in cattle “a review”. Anim. Reprod. Sci., 155: 11–27.
Search in Google Scholar Back to article
Nagashima J.B., Sylvester S.R., Nelson J.L., Cheong S.H., Mukai C., Lambo C., Flanders J.A., Meyers-Wallen V.N., Songsasen N., Travis A.J. (2015). Live births from domestic dog (Canis familiaris) embryos produced by in vitro fertilization. Plos One, 10: e0143930.
Search in Google Scholar Back to article
Nagashima J.B., Travis A.J., Songsasen N. (2019) The domestic dog embryo: in vitro fertilization, culture, and transfer. Methods Mol. Biol., 2006: 247–267.
Search in Google Scholar Back to article
Niskanen M., Thrusfield M.V. (1998). Associations between age, parity, hormonal therapy and breed, and pyometra in Finnish dogs. Vet. Rec., 143: 493–498.
Search in Google Scholar Back to article
Palomino J., De Los Reyes M. (2016). Temporal expression of GDF-9 and BMP-15 mRNAs in canine ovarian follicles. Theriogenology, 86: 1541–1549.
Search in Google Scholar Back to article
Paulini F., Melo E.O. (2011). The role of oocyte-secreted factors GDF9 and BMP15 in follicular development and oogenesis. Reprod. Domest. Anim., 46: 354–361.
Search in Google Scholar Back to article
Samiec M., Skrzyszowska M. (2013). Assessment of in vitro developmental capacity of porcine nuclear-transferred embryos reconstituted with cumulus oophorus cells undergoing vital diagnostics for apoptosis detection. Ann. Anim. Sci., 13: 513–529.
Search in Google Scholar Back to article
Samiec M., Romanek J., Lipinski D., Opiela J. (2019). Expression of pluripotency-related genes is highly dependent on trichostatin A-assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos. Anim. Sci. J., 90: 1127–1141.
Search in Google Scholar Back to article
Santos T.A., El Shourbagy S., St John J.C. (2006). Mitochondrial content reflects oocyte variability and fertilization outcome. Fertil. Steril., 85: 584–591.
Search in Google Scholar Back to article
Songsasen N., Wildt D.E. (2005). Size of the donor follicle, but not stage of reproductive cycle or seasonality, influences meiotic competency of selected domestic dog oocytes. Mol. Reprod. Dev., 72: 113–119.
Search in Google Scholar Back to article
Turathum B., Saikhun K., Sangsuwan P., Kitiyanant Y. (2010). Effects of vitrification on nuclear maturation, ultrastructural changes and gene expression of canine oocytes. Reprod. Biol. Endocrinol., 8: 70.
Search in Google Scholar Back to article
Van Soom A., Rijsselaere T., Filliers M. (2014). Cats and dogs: two neglected species in this era of embryo production in vitro? Reprod. Domest. Anim., 49, Suppl 2: 87–91.
Search in Google Scholar Back to article
Wai T., Ao A., Zhang X., Cyr D., Dufort D., Shoubridge E.A. (2010). The role of mitochondrial DNA copy number in mammalian fertility. Biol. Reprod., 83: 52–62.
Search in Google Scholar Back to article
Zuccotti M., Merico V., Bellone M., Mulas F., Sacchi L., Rebuzzini P., Prigione A., Redi C.A., Bellazzi R., Adjaye J., Garagna S. (2011). Gatekeeper of pluripotency: a common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells. BMC Genomics, 12: 1–13.
Search in Google Scholar Back to article
Zuccotti M., Merico V., Belli M., Mulas F., Sacchi L., Zupan B., Redi C.A., Prigione A., Adjaye J., Bellazzi R., Garagna S. (2012). OCT4 and the acquisition of oocyte developmental competence during folliculogenesis. Int. J. Dev. Biol., 56: 853–858.
Search in Google Scholar Back to article

Pyometra does not affect some molecular quality-related parameters of canine oocytes

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