Developmental Competence of CMV-Fut Transgenic and Non-Transgenic Pig Embryos Cultured in Vitro / Potencjał Rozwojowy CMV-Fut Transgenicznych I Nietransgenicznych Zarodków Świni Hodowanych In Vitro

Jura, Jacek; Smorąg, Zdzisław; Gajda, Barbara; Lipiński, Daniel; Słomski, Ryszard

doi:10.2478/aoas-2013-0051

Abstract

Possible influence of a transgene on life functions of embryos makes it reasonable to confirm or deny it for a particular gene construct. In vitro development of an embryo is a widely used criterion of its competence. The aim of the study was to compare in vitro developmental capacity of transgenic and non-transgenic pig embryos. The results showed a statistically significant difference in in

vitro developmental capacity of embryos obtained from transgenic and non-transgenic pigs. Developmental competence of embryos (morula and blastocyst stage) produced from zygotes obtained from transgenic sows decreased compared to that obtained from non-transgenic sows.

References

Bryła M., Trzcińska M., Wieczorek J., Słomski R., Smorag Z. (2010). Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos. Anim. Reprod. Sci., 118: 77-82.
Search in Google Scholar
Dang - Nguyen T.Q., Kikuchi K., Somfai T., Ozawa M., Nakai M., Maedomari N., Viet - Linh N., Kanai Y., Nguyen B.X., Nagai T. (2010). Evaluation of developmental competence of in vitro-produced porcine embryos based on the timing, pattern and evenness of the first cleavage and onset of the second cleavage. J. Reprod. Dev., 56: 593-600.
Search in Google Scholar
Gajda B. (2009). Factors and methods of pig oocyte and embryo quality improvement and their application in reproductive biotechnology. Reprod. Biol., 9: 97-112.
Search in Google Scholar
Jura J., Smorąg Z., Gajda B., Jurkiewicz J. (2004). Production of F1 and F2 generations of WAP-FUCtransgenic pigs. Molecular analysis. Ann. Anim. Sci., 4: 215-222.
Search in Google Scholar
Jura J., Smorąg Z., Słomski R., Lipiński D., Gajda B. (2007). Factors affecting the production of potential transgenic pigs by DNAmicroinjection;asix-year retrospective study. J. Anim. Feed Sci., 16: 641-650.
Search in Google Scholar
Lipiński D., Jura J., Zeyland J., Juzwa W., Mały E., Kalak R., Bochenek M., Plawski A., Szalata M., Smorąg Z., Słomski R. (2010). Production of transgenic pigs expressing human α1,2-fucosyltransferase to avoid humoral xenograft rejection. Med. Weter., 66: 316-322. Petters R.M., Wells K.D. (1993). Culture of pig embryos. J. Reprod. Fertil., 48 (Suppl.): 61-73.
Search in Google Scholar
Samiec M., Skrzyszowska M. (2011). Transgenic mammalian species, generated by somatic cell cloning, in biomedicine, biopharmaceutical industry and human nutrition/dietetics - recent achievements. Pol. J. Vet. Sci., 14: 317-328.
Search in Google Scholar
Skrzyszowska M., Samiec M., Słomski R., Lipiński D., Mały E. (2008). Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection. Theriogenology, 70: 248-259.
Search in Google Scholar

Developmental Competence of CMV-Fut Transgenic and Non-Transgenic Pig Embryos Cultured in Vitro / Potencjał Rozwojowy CMV-Fut Transgenicznych I Nietransgenicznych Zarodków Świni Hodowanych In Vitro

Abstract

Paradigm

My account