Identification of Telomeric Sequences in Pigs with Rearranged Karyotype Using Prins Technique / Identyfikacja sekwencji telomerowych u świń z rearanżacją kariotypu przy wykorzystaniu techniki PRINS

Danielak-Czech, Barbara; Rejduch, Barbara; Kozubska-Sobocińska, Anna

doi:10.2478/aoas-2013-0022

Abstract

Using PRINS technique with oligonucleotide primer complementary to the telomere sequence repeats (TTAGGG )n, the interstitial telomeric signals in 6q22 and 7q13 unstable chromosome regions in pigs with t(7;13)(q13;q46) reciprocal translocation were identified. The intrachromosomal localization of telomeric repeats specific for 7q13 region were revealed to strictly correspond to breakpoint of this translocation as well as several others described earlier. The present study showed the usefulness of PRINS technique for identification of intrachromosomal telomeric sequences in pig genome regions, extremely prone to breakage and predisposed to chromosome reorganizations, especially with regard to the clinical cases of karyotype defects.

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Identification of Telomeric Sequences in Pigs with Rearranged Karyotype Using Prins Technique / Identyfikacja sekwencji telomerowych u świń z rearanżacją kariotypu przy wykorzystaniu techniki PRINS

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