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Quantitative Real-Time PCR testing in the control of hepatitis B and C: Progress and challenges towards eradication by 2030 Cover

Quantitative Real-Time PCR testing in the control of hepatitis B and C: Progress and challenges towards eradication by 2030

By: Iulia Patrascu  
Open Access
|Dec 2025

References

  1. CDC. Global Viral Hepatitis. Viral Hepat. 2024 [cited 2025 July 21]. Available from: https://www.cdc.gov/hepatitis/global/index.html
  2. VA.gov | Veterans Affairs. [cited 2025 July 23]. Available from: https://www.hepatitis.va.gov/hcv/patient/diagnosis/labtests-RNA-quantitative-testing.asp?utm_source=perplexity
  3. Ghany MG, Strader DB, Thomas DL, Seeff LB, American Association for the Study of Liver Diseases. Diagnosis, management, and treatment of hepatitis C: an update. Hepatol Baltim Md. 2009;49:1335–74.
  4. Firdaus R, Saha K, Biswas A, Sadhukhan PC. Current molecular methods for the detection of hepatitis C virus in high risk group population: A systematic review. World J Virol. 2015;4:25–32.
  5. Bag S, Ghosh S, Lalwani R, Vishnoi P, Gupta S, Dubey D. Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA. J Vis Exp JoVE. 2023;
  6. Yang JH, Lai JP, Douglas SD, Metzger D, Zhu XH, Ho WZ. Real-time RT-PCR for quantitation of hepatitis C virus RNA. J Virol Methods. 2002;102:119–28.
  7. Caliendo AM, Valsamakis A, Bremer JW, Ferreira-Gonzalez A, Granger S, Sabatini L, et al. Multilaboratory Evaluation of Real-Time PCR Tests for Hepatitis B Virus DNA Quantification. J Clin Microbiol. 2011;49:2854–8.
  8. Chen L, Li W, Zhang K, Zhang R, Lu T, Hao M, et al. Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy. J Mol Diagn JMD. 2016;18:84–91.
  9. Vesper HW, Myers GL, Miller WG. Current practices and challenges in the standardization and harmonization of clinical laboratory tests123. Am J Clin Nutr. 2016;104:907S-912S.
  10. Lampertico P, Agarwal K, Berg T, Buti M, Janssen HLA, Papatheodoridis G, et al. EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection. J Hepatol. 2017;67:370–98.
  11. Hepatitis C Guidance 2019 Update: AASLD-IDSA Recommendations for Testing, Managing, and Treating Hepatitis C Virus Infection. Hepatol Baltim Md. 2020;71:686–721.
  12. Guidelines for the prevention, diagnosis, care and treatment for people with chronic hepatitis B infection. [cited 2025 Sept 13]. Available from: https://www.who.int/publications/i/item/9789240090903
  13. Updated recommendations on simplified service delivery and diagnostics for hepatitis C infection. [cited 2025 Sept 13]. Available from: https://www.who.int/publications/i/item/9789240052697
  14. European Association for the Study of the Liver. EASL Clinical Practice Guidelines on the management of hepatitis B virus infection. J Hepatol. 2025;83:502–83.
  15. Terrault NA, Lok ASF, McMahon BJ, Chang K-M, Hwang JP, Jonas MM, et al. Update on prevention, diagnosis, and treatment of chronic hepatitis B: AASLD 2018 hepatitis B guidance. Hepatol Baltim Md. 2018;67:1560–99.
  16. Falla AM, Veldhuijzen IK, Ahmad AA, Levi M, Hendrik Richardus J. Limited access to hepatitis B/C treatment among vulnerable risk populations: an expert survey in six European countries. Eur J Public Health. 2017;27:302–6.
  17. Bajis S, Dore GJ, Hajarizadeh B, Cunningham EB, Maher L, Grebely J. Interventions to enhance testing, linkage to care and treatment uptake for hepatitis C virus infection among people who inject drugs: A systematic review. Int J Drug Policy. 2017;47:34–46.
  18. Coppola N, Alessio L, De Pascalis S, Macera M, Di Caprio G, Messina V, et al. Effectiveness of test-and-treat model with direct-acting antiviral for hepatitis C virus infection in migrants: a prospective interventional study in Italy. Infect Dis Poverty. 2024;13:39.
  19. Khalesi Z, Razizadeh MH, Javadi M, Bahavar A, Keyvanlou Z, Saadati H, et al. Global epidemiology of HBV infection among hemodialysis patients: A systematic review and meta-analysis. Microb Pathog. 2023;179:106080.
  20. Narh Lasidji B, Senoo-Dogbey VE, Appiah IK. Analysis of factors associated with hepatitis B and C exposure and reporting patterns among healthcare workers in a tertiary care facility: a cross-sectional study. BMJ Open. 2025;15:e101193.
  21. Stein JA, Andersen RM, Robertson M, Gelberg L. Impact of Hepatitis B and C Infection on Health Services Utilization in Homeless Adults: A Test of the Gelberg-Andersen Behavioral Model for Vulnerable Populations. Health Psychol. 2012;31:20–30.
  22. Jia J, Zhu Q, Deng L, Lan G, Johnson A, Chen H, et al. Treatment outcomes of HIV patients with hepatitis B and C virus co-infections in Southwest China: an observational cohort study. Infect Dis Poverty. 2022;11:7.
  23. Wu S, Wang J, Guo Q, Lan H, Sun Y, Ren M, et al. Prevalence of human immunodeficiency virus, syphilis, and hepatitis B and C virus infections in pregnant women: a systematic review and meta-analysis. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. 2023;29:1000–7.
  24. Huynh TT-T, Ha AT, Nguyen NT, Vu YT-H, Nguyen DH, Ha TM, et al. Diagnostic Accuracy of Plate-Based Digital PCR Assays for Simultaneous Quantitation of Hepatitis B and D Viral Loads in Patients’ Serum. Asian Pac J Cancer Prev APJCP. 2025;26:2079–86.
  25. Zhang Q, Cai D-C, Hu P, Ren H. Low-level viremia in nucleoside analog-treated chronic hepatitis B patients. Chin Med J (Engl). 2021;134:2810–7.
  26. Raimondo G, Locarnini S, Pollicino T, Levrero M, Zoulim F, Lok AS, et al. Update of the statements on biology and clinical impact of occult hepatitis B virus infection. J Hepatol. 2019;71:397–408.
  27. Kwak M-S, Kim YJ. Occult hepatitis B virus infection. World J Hepatol. 2014;6:860–9.
  28. Farshadpour F, Taherkhani R, Saberi F. Molecular evaluation of hepatitis B virus infection and predominant mutations of pre-core, basal core promoter and S regions in an Iranian population with type 2 diabetes mellitus: a case–control study. BMC Infect Dis. 2022;22:553.
  29. Zauli DAG, Menezes CLPD, Oliveira CLD, Mateo ECC, Ferreira ACDS. In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections. Braz J Microbiol. 2016;47:987–92.
  30. Liang J, Liang X, Ma H, Nie L, Tian Y, Chen G, et al. Detection of Hepatitis B Virus M204V Mutation Quantitatively via Real-time PCR. J Clin Transl Hepatol. 2021;9:143–8.
  31. Vázquez-Morón S, Ardizone Jiménez B, Jiménez-Sousa MA, Bellón JM, Ryan P, Resino S. Evaluation of the diagnostic accuracy of laboratory-based screening for hepatitis C in dried blood spot samples: A systematic review and meta-analysis. Sci Rep. 2019;9:7316.
  32. Zangiabadian M, Zamani A, Nasiri MJ, Behzadi E, Fooladi AAI. Diagnostic Accuracy and Validity of Serological and Molecular Tests for Hepatitis B and C. Curr Pharm Biotechnol. 2022;23:803–17.
  33. Lai JC-T, Wong GL-H, Tse Y-K, Hui VW-K, Lai MS-M, Chan HL-Y, et al. Histological severity, clinical outcomes and impact of antiviral treatment in indeterminate phase of chronic hepatitis B: A systematic review and meta-analysis. J Hepatol. 2025;82:992–1003.
  34. Xie L, Liu G, Liu Y, Li P, Guo H. Prevalence of low-level viremia in the treatment of chronic hepatitis B in China: a systematic review and meta-analysis. BMJ Open. 2025;15:e088756.
  35. Allweiss L, Testoni B, Yu M, Lucifora J, Ko C, Qu B, et al. Quantification of the hepatitis B virus cccDNA: evidence-based guidelines for monitoring the key obstacle of HBV cure. Gut. 2023;72:972–83.
  36. Zhang H, Tu T. Approaches to quantifying hepatitis B virus covalently closed circular DNA. Clin Mol Hepatol. 2022;28:135–49.
  37. Konstantinou D, Deutsch M. The spectrum of HBV/HCV coinfection: epidemiology, clinical characteristics, viralinteractions and management. Ann Gastroenterol Q Publ Hell Soc Gastroenterol. 2015;28:221–8.
  38. Singh KP, Crane M, Audsley J, Avihingsanon A, Sasadeusz J, Lewin SR. HIV-hepatitis B virus coinfection: epidemiology, pathogenesis, and treatment. AIDS Lond Engl. 2017;31:2035–52.
  39. Hossain MG, Ueda K. A meta-analysis on genetic variability of RT/HBsAg overlapping region of hepatitis B virus (HBV) isolates of Bangladesh. Infect Agent Cancer. 2019;14:33.
DOI: https://doi.org/10.2478/amma-2025-0046 | Journal eISSN: 2668-7763 | Journal ISSN: 2668-7755
Language: English
Page range: 284 - 289
Submitted on: Aug 11, 2025
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Accepted on: Sep 15, 2025
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Published on: Dec 11, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Iulia Patrascu, published by University of Medicine, Pharmacy, Science and Technology of Targu Mures
This work is licensed under the Creative Commons Attribution 4.0 License.