Surgical procedure for acellular vascular xenografts testing in sheep carotid artery

Marius Mihai Harpa; Alexandra Iulia Puscas; Emanuel David Anitei; Dan Teodor Simionescu; Raluca Ionela Truta; Hamida Al-Hussein; Ionela Cotfas; Claudiu Ghiragosian; Cosmin Marian Banceu; Tudor Capilna; Horatiu Suciu; Hussam Al-Hussein

doi:10.2478/amma-2024-0024

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Surgical procedure for acellular vascular xenografts testing in sheep carotid artery

Acta Marisiensis - Seria Medica

Volume 70 (2024): Issue 3 (September 2024)

By: Marius Mihai Harpa, Alexandra Iulia Puscas, Emanuel David Anitei, Dan Teodor Simionescu, Raluca Ionela Truta, Hamida Al-Hussein, Ionela Cotfas, Claudiu Ghiragosian, Cosmin Marian Banceu, Tudor Capilna, Horatiu Suciu and Hussam Al-Hussein

Open Access

|Oct 2024

Abstract

Objective

The objective of this study is to demonstrate the safety and reproducibility of our surgical technique for implanting decellularized vascular xenografts in the carotid artery of sheep.

Methods

Acellular porcine carotid arteries were implanted as interposition xenografts in seven sheep. An intravascular shunt was used for cerebral protection, and a flowmeter was utilized to assess graft performance.

Results

There were no intraoperative deaths or postoperative neurological complications observed. Acute graft thrombosis occurred in one sheep during surgery, but was successfully managed with thrombectomy to restore blood flow. Post-implantation flowmetry and Doppler ultrasound confirmed graft functionality.

Conclusions

Our study demonstrates the successful application of our surgical method for implanting decellularized vascular xenografts in the carotid artery of sheep. The implanted grafts maintained patency, normal blood flow, and favorable wound healing and neurological outcomes post-surgery.

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Articles in this issue

DOI: https://doi.org/10.2478/amma-2024-0024 | Journal eISSN: 2668-7763 | Journal ISSN: 2668-7755

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Language: English

Page range: 118 - 121

Submitted on: Jul 15, 2024

Accepted on: Jul 20, 2024

Published on: Oct 2, 2024

Published by: University of Medicine, Pharmacy, Science and Technology of Targu Mures

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Related subjects:

Clinical medicine, other

© 2024 Marius Mihai Harpa, Alexandra Iulia Puscas, Emanuel David Anitei, Dan Teodor Simionescu, Raluca Ionela Truta, Hamida Al-Hussein, Ionela Cotfas, Claudiu Ghiragosian, Cosmin Marian Banceu, Tudor Capilna, Horatiu Suciu, Hussam Al-Hussein, published by University of Medicine, Pharmacy, Science and Technology of Targu Mures
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 70 (2024): Issue 3 (September 2024)