Purple sweet potato (Ipomoea batatas L.) reduces the wound temperature and erythema in STZ-induced diabetic rats

Suprapto, Ratih Paramita; Kristianto, Heri

doi:10.2478/amma-2024-0018

Abstract

Objective

Wound temperature is one of the wound biomarkers representing the wound healing progress. The diabetic wound is associated with a prolonged inflammation phase marked by an increasing wound temperature and erythematous wound. Purple sweet potato extract (PSP), an anthocyanin-rich plant, improved wound healing in both diabetic and non-diabetic wounds in animal studies. This study aims to know the effect of purple sweet potato ethanol extract on wound temperature and erythema in streptozotocin-induced diabetic rats.

Methods

Rats were divided into four groups: normal rats + gel placebo; diabetic rats + gel placebo; diabetic rats + PSP 10%; and diabetic rats + PSP 15%. Diabetes mellitus was induced by streptozotocin injection. After diabetic confirmation, the back of the rats was excised and the gel was administered daily for 14 days. The wound temperature was measured at the wound surface using infrared thermography on days 0, 3, 7, and 14. The wounds were photographed and erythema analysis was conducted using Corel Photo paint®.

Results

Diabetic wounds exhibited higher surface temperature compared with the normal wound (37.08°C ± 0.29 vs 35.13°C ± 0.46) on day 14 of examination and topical application the purple sweet potato ethanol extract gel 10% and 15% markedly decreased the wound temperature at day 7 and 14 compared with the diabetes wound (p<0.0001). Wound erythema was significantly reduced in the PSP 10% and 15% diabetic wound treatment groups on day 14 (p<0.05).

Conclusion

Purple sweet potato extract gel treatment was found to have the potential to reduce inflammation in diabetic wounds.

References

Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci. 2016;73(20):3861–85.
Search in Google Scholar Back to article
Dasari N, Jiang A, Skochdopole A, Chung J, Reece EM, Vorstenbosch J, et al. Updates in Diabetic Wound Healing, Inflammation, and Scarring. Semin Plast Surg. 2021;35(3):153–8.
Search in Google Scholar Back to article
Gethin G, O’Connor GM, Abedin J, Newell J, Flynn L, Watterson D, et al. Monitoring of pH and temperature of neuropathic diabetic and nondiabetic foot ulcers for 12 weeks: An observational study. Wound Repair Regen. 2018;26(2):251–6.
Search in Google Scholar Back to article
Lavery LA, Petersen BJ, Linders DR, Bloom JD, Rothenberg GM, Armstrong DG. Unilateral remote temperature monitoring to predict future ulceration for the diabetic foot in remission. BMJ Open Diabetes Res Care. 2019;7(1).
Search in Google Scholar Back to article
de Araújo AL, Negreiros FD da S, Florêncio RS, de Oliveira SKP, da Silva ARV, Moreira TMM. Effect of thermometry on the prevention of diabetic foot ulcers: a systematic review with meta-analysis*. Rev Lat Am Enfermagem. 2022;30.
Search in Google Scholar Back to article
Perciun ER, Buse AM. The Handheld Infrared Thermometry in the Diabetic Foot-Useful but Debatable Technique. Rom J Diabetes, Nutr Metab Dis. 2020;27(3):298–302.
Search in Google Scholar Back to article
Andreas Neubauer and Harald Schnidar. Methods for assessing erythema [Internet]. Vol. 1. 2014. p. 1–49. Available from: https://patents.google.com/patent/EP2976013A1/en
Search in Google Scholar Back to article
Albahri G, Badran A, Hijazi A, Daou A, Baydoun E, Nasser M, et al. The Therapeutic Wound Healing Bioactivities of Various Medicinal Plants. Life. 2023;13(2).
Search in Google Scholar Back to article
Sugata M, Lin CY, Shih YC. Anti-Inflammatory and Anticancer Activities of Taiwanese Purple-Fleshed Sweet Potatoes (Ipomoea batatas L. Lam) Extracts. Biomed Res Int. 2015;2015.
Search in Google Scholar Back to article
Ningrum DD, Padaga MC, Fauzi A. The effect of purple sweet potato (Ipomea batatas L.) extract gel toward TNF-α expression and skin collagen density on rats (Rattus norvegicus) with open wound models. J Phys Conf Ser. 2020;1430(1).
Search in Google Scholar Back to article
Kristianto H, Nurachmah E, Gayatri D, Geraghty T, LaPorta G, Singh R, et al. The effect of purple sweet potato (Ipomea batatas L.) extract gel toward TNF-α expression and skin collagen density on rats (Rattus norvegicus) with open wound models. Antioxidants [Internet]. 2021;13(1):1–14. Available from: http://dx.doi.org/10.1038/nm.3887%5Cnhttp://www.ncbi.nlm.nih.gov/pubmed/26076037%5Cn http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4631120
Search in Google Scholar Back to article
Panda V, Sonkamble M PS. Functional Foods in Health and Diseases. Funct Foods Heal Dis. 2011;1(10):403–15.
Search in Google Scholar Back to article
Dewi KN, Kristianto H, Indra MR. The Effect of Squid Extract (Loligo Sp) on TNF-α and TGF-β1 Serum Levels during Wound Healing in Streptozotocin-induced Diabetic Rats. Makara J Heal Res. 2016;20(3).
Search in Google Scholar Back to article
Sutejo IR, Hasanah AN, Sudarko FR. Ethanolic extract of Edamame (Glycine max L. merril) enhance second degree burn wound healing trough modulating of hydroxiproline levels and increasing epithelial thickness. Acta Marisiensis - Ser Medica. 2022;68(2):55–60.
Search in Google Scholar Back to article
Atiba A, Nishimura M, Kakinuma S, Hiraoka T, Goryo M, Shimada Y, et al. Aloe vera oral administration accelerates acute radiation-delayed wound healing by stimulating transforming growth factor-β and fibroblast growth factor production. Am J Surg [Internet]. 2011;201(6):809–18. Available from: http://dx.doi.org/10.1016/j.amjsurg.2010.06.017
Search in Google Scholar Back to article
Alam FM, Kurnianingsih N, Fatchiyah F. Phytochemical Analysis of Purple Sweet Potatoes (Ipomoea batatas) Roots Extract From Lawang and Kawi Mountain Cultivar, East Java, Indonesia. J Exp Life Sci. 2022;12(1):17–22.
Search in Google Scholar Back to article
Komorowska P, Różańska S, Różański J. Effect of the degree of substitution on the rheology of sodium carboxymethylcellulose solutions in propylene glycol/water mixtures. Cellulose. 2017;24(10):4151–62.
Search in Google Scholar Back to article
Kristianto H, Nurachmah E, Gayatri D. Peningkatan Ekspresi Transforming Growth Factor Beta 1 (TGF β1) Pada Luka Diabetes Melitus Melalui Balutan Modern. J Keperawatan Indones. 2010;13(1):20–5.
Search in Google Scholar Back to article
Bus SA. Innovation in plantar pressure and foot temperature measurements in diabetes. Diabetes Metab Res Rev. 2016;32(Suppl. 1):221–6.
Search in Google Scholar Back to article
Sendangratri, Handayani R, Elya B. Inhibitory effects of different varieties of sweet potato (Ipomoea batatas L.) tubers extracts on lipoxygenase activity. Pharmacogn J. 2019;11(6):1195–8.
Search in Google Scholar Back to article