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From Fleas to T Lymphocytes and Beyond: How did I Become an Immunologist, and What Has Resulted from It? Cover

From Fleas to T Lymphocytes and Beyond: How did I Become an Immunologist, and What Has Resulted from It?

By: Paweł Kisielow  
Open Access
|Aug 2025

References

  1. Allen PM, Strydom DJ, Unanue ER (1984) Processing of lysozyme by macrophages: identification of the determinant recognized by two T-cell hybridomas. Proc Natl Acad Sci USA 81:2489–2493. https://doi.org/10.1073/pnas.81.8.2489
  2. Bevan MJ (1977) In a radiation chimaera, host H-2 antigens determine immune responsiveness of donor cytotoxic cells. Nature 269:417–418. https://doi.org/10.1038/269417a0
  3. Billingham RE, Brent L, Medawar PB (1953) Actively acquired tolerance of foreign cells. Nature 172:603–606. https://doi.org/10.1038/172603a0
  4. Bjorkman PJ, Saper MA, Samraoui B et al. (1987) The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens. Nature 329:512–518. https://doi.org/10.1038/329512a0
  5. Boyse EA, Miyazawa M, Aoki T et al. (1968) Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc Lond B Biol Sci 170:175–193. https://doi.org/10.1098/rspb.1968.0032
  6. Burnet FM (1957) A modification of Jerne’s theory of antibody production using the concept of clonal selection. Aust J Sci 20:67–69.
  7. Cammarota G, Scheirle A, Takacs B et al. (1992) Identification of a CD4 binding site on the beta 2 domain of HLA-DR molecules. Nature 356:799–801. https://doi.org/10.1038/356799a0
  8. Cantor H, Boyse EA (1975a) Functional subclasses of T lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med 141:1376–1389. https://doi.org/10.1084/jem.141.6.1376
  9. Cantor H, Boyse EA (1975b) Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med 141:1390–1399. https://doi.org/10.1084/jem.141.6.1390
  10. Ceredig R, MacDonald HR, Jenkinson EJ (1983) Flow microfluorometric analysis of mouse thymus development in vivo and in vitro. Eur J Immunol 13:185–190. https://doi.org/10.1002/eji.1830130302
  11. Cooper MD, Peterson RD, Good RA (1965) Delineation of the thymic and bursal lymphoid systems in the chicken. Nature 205:143–146. https://doi.org/10.1038/205143a0
  12. Cullen SE, David CS, Shreffler DC et al. (1974) Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci USA 71:648–652. https://doi.org/10.1073/pnas.71.3.648
  13. David CS, Shreffler DC, Frelinger JA (1973) New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. Proc Natl Acad Sci USA 70:2509–2514. https://doi.org/10.1073/pnas.70.9.2509
  14. Dembić Z, Haas W, Weiss S et al. (1986) Transfer of specificity by murine alpha and beta T-cell receptor genes. Nature 320:232–238. https://doi.org/10.1038/320232a0
  15. Dialynas DP, Wilde DB, Marrack P et al. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK 1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev 74:29–56. https://doi.org/10.1111/j.1600-065x.1983.tb01083.x
  16. Ehrlich P (1900) Croonian lecture – On immunity with special reference to cell life. Proc R Soc Lond 66:424448. https://doi.org/10.1098/rspl.1899.0121
  17. Erb P, Feldman M (1975) The role of macrophages in the generation of T-helper cells II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. J Exp Med 142:460–472. https://doi.org/10.1084/jem.142.2.460
  18. Gowans JL, McGregor DD, Cowen DM et al. (1962) Initiation of immune responses by small lymphocytes. Nature 196:651–655. https://doi.org/10.1038/196651a0
  19. Haskins K, Kubo R, White J et al. (1983) The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med 157:1149–1169. https://doi.org/10.1084/jem.157.4.1149
  20. Hedrick SM, Cohen DI, Nielsen EA et al. (1984) Isolation of cDNA clones encoding T cell-specific membrane-associated proteins. Nature 308:149–153. https://doi.org/10.1038/308149a0
  21. Hozumi N, Tonegawa S (1976) Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc Natl Acad Sci USA 73:3628–3632. https://doi.org/10.1073/pnas.73.10.3628
  22. Jaenisch R, Mintz B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71:1250–1254. https://doi.org/10.1073/pnas.71.4.1250
  23. Kaye J, Hsu ML, Sauron ME et al. (1989) Selective development of CD4+ T cell in transgenic mice expressing a class II MHC-restricted antigen receptor. Nature 341:746–749. https://doi.org/10.1038/341746a0
  24. Kisielow P (1970) Growth in vitro of L-1210 leukemia in suspension culture. Arch Immunol Ther Exp 18:491–499.
  25. Kisielow P (2014) Demonstration of functional heterogeneity of T lymphocytes and identification of their two major subsets. Front Immunol 5:609. https://doi.org/10.3389/fimmu.2014.00609
  26. Kisielow P, Blüthmann H, Staerz UD et al. (1988a) Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4+8+ thymocytes. Nature 333:742–746. https://doi.org/10.1038/333742a0
  27. Kisielow P, Hirst JA, Shiku H et al. (1975) Ly antigens as markers for functionally distinct subpopulations of thymus-derived lymphocytes of the mouse. Nature 253:219–220. https://doi.org/10.1038/253219a0
  28. Kisielow P, Leiserson W, von Boehmer H (1984) Differentiation of thymocytes in fetal organ culture: analysis of phenotypic changes accompanying the appearance of cytolytic and interleukin-2-producing cells. J Immunol 133:1117–1123. https://doi.org/10.4049/jimmunol.133.3.1117
  29. Kisielow P, Miazek A (1995) Positive selection of T cells: rescue from programmed cell death and differentiation require continual engagement of the T cell receptor. J Exp Med 181:1975–1984. https://doi.org/10.1084/jem.181.6.1975
  30. Kisielow P, Teh HS, Blüthmann H et al. (1988b) Positive selection antigen-specific T cells in thymus by restricting MHC molecules. Nature 335:730–733. https://doi.org/10.1038/335730a0
  31. Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497. https://doi.org/10.1038/256495a0
  32. Lamb CA, Yewdell JW, Bennink JR et al. (1991) Invariant chain targets HLA class II molecules to acidic endosomes containing internalized influenza virus. Proc Natl Acad Sci USA 88: 5998–6002. https://doi.org/10.1073/pnas.88.14.5998
  33. Lederberg J (1959) Genes and antibodies. Science 129:1649–1653. https://doi.org/10.1126/science.129.3364.164
  34. McIntyre BW, Allison JP (1983) The mouse T cell receptor: structural heterogeneity of molecules of normal T cells defined by xenoantiserum. Cell 34:739–746. https://doi.org/10.1016/0092-8674(83)90530-5
  35. Medawar PB, Simpson E (1975) Thymus-dependent lymphocytes. Nature 258:106–107. https://doi.org/10.1038/258106a0
  36. Miller JF (1961) Immunological function of the thymus. Lancet 2:748–749. https://doi.org/10.1016/s0140-6736(61)90693-6
  37. Miller JF, Mitchell GF (1967) The thymus and the precursors of antigen-reactive cells. Nature 216:659–663. https://doi.org/10.1038/216659a0
  38. Miller JFAP, Sadelain M (2015) The journey from discoveries in fundamental immunology to cancer immunotherapy. Cancer Cell 27:439–449. https://doi.org/10.1016/j.ccell.2015.03.007
  39. Nuchtern JG, Bonifacino JS, Biddison WE et al. (1989) Brefeldin A implicates egress from endoplasmic reticulum in class I restricted antigen presentation. Nature 339:223–226. https://doi.org/10.1038/339223a0
  40. Reinherz EL, Kung PC, Goldstein G et al. (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061–4065. https://doi.org/10.1073/pnas.76.8.4061
  41. Sakaguchi S (2004) Naturally arising CD4+ regulatory T cells for immunologic self-tolerance and negative control of immune responses. Annu Rev Immunol 22:531–562. https://doi.org/10.1146/annurev.immunol.21.120601.141122
  42. Salter RD, Benjamin RJ, Weseley PK et al. (1990) A binding site for the T-cell co-receptor CD8 on the alpha 3 domain of HLA-A2. Nature 345:41–46. https://doi.org/10.1038/345041a0
  43. Shortman K, Jackson H (1974) The differentiation of T lymphocytes. I. Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells. Cell Immunol 12:230–246. https://doi.org/10.1016/0008-8749(74)90075-6
  44. Siku H, Kisielow P, Bean MA et al. (1975) Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells. J Exp Med 141:227–241. https://doi.org/10.1084/jem.141.1.227
  45. Swat W, Ignatowicz L, Boehmer H et al. (1991) Clonal deletion of immature CD4+8+ thymocytes in suspension culture by extrathymic antigen-presenting cells. Nature 351:150–153. https://doi.org/10.1038/351150a0
  46. Teh HS, Kisielow P, Scott B et al. (1988) Thymic major histocompatibility complex antigens and the αβ T-cell receptor determine CD4/CD8 phenotype of T cells. Nature 335:229–233. https://doi.org/10.1038/335229a0
  47. Watson J (1962) James Watson - Biographical. https://www.Nobelprize.org/prizes/medicine/1962/Watson/biographical/
  48. Yanagi Y, Yoshikai Y, Legett K et al. (1984) A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature 308:145–149. https://doi.org/10.1038/308145a0
  49. Zinkernagel RM (1978) Thymus and lymphohemopoietic cells: their role in T cell maturation in selection of T cells’ H-2-restriction-specificity and in H-2 linked Ir gene control. Immunol Rev 42:224–270. https://doi.org/10.1111/j.1600-065x.1978.tb00264.x
  50. Zinkernagel RM, Doherty PC (1974) Restriction of in vitro T cell-mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system. Nature 248:701–702. https://doi.org/10.1038/248701a0
Language: English
Submitted on: Jun 24, 2025
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Accepted on: Jul 23, 2025
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Published on: Aug 22, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2025 Paweł Kisielow, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.